Abstract
A total of 18 strains of marine endophytic fungi were fermented by three fermentation media with significantly different carbon sources, nitrogen sources and growth factors, which was gotten from 54 fermentation products. These 54 fermentation products were evaluated through chemical screening and bioactivity screening. The ethyl acetate extract of 18 strains of marine endophytic fungus was screened by HPLC, antioxidant activity in vitro and antibacterial activits. Ethyl acetate extract (10 mg/mL) of the strain 150103 (medium 1) could inhibit bacillus subtilis and Staphylococcus aureus effectively. The diameter of inhibition zone is 12 mm and 11 mm respectively. It couldn’t inhibit Escherichia coli evidently. It could remove more than 90% of DPPH free radicals. According to the HPLC results, extract of the Strain 150103 (medium 1) is rich of secondary metabolites. The Strain 150103 (medium 1) is rich of secondary metabolites, has strong antibacterial and antioxidant activity in vitro, and deserves deep research.
Publication Date
2-28-2016
First Page
41
Last Page
43,74
DOI
10.13652/j.issn.1003-5788.2016.02.010
Recommended Citation
Binzhi, SUN; Zhenhua, TIAN; and Xu, LIU
(2016)
"Preliminary screening and biological activity of fermentation products of 18 marine endophytic fungi strains,"
Food and Machinery: Vol. 32:
Iss.
2, Article 10.
DOI: 10.13652/j.issn.1003-5788.2016.02.010
Available at:
https://www.ifoodmm.cn/journal/vol32/iss2/10
References
[1] Kleoppe J W, Schipper B, Bakker P. Proposed elimination of the term endorhizosphere[J]. Phytopatholology, 1992, 82: 726-727.
[2] Stierle A, Strobel G, Stierle D. Taxol and taxane production by Taxomyces andreanae,an endophytic fungus of pacific yew[J]. Science, 1993, 260: 214-216.
[3] Joseph B, Priya R M. Bioactive compound from endophytes and their potential in pharmaceutical effect: a review[J]. American Journal of Biochemistry and Molecular Biology, 2011, 1(3): 291-309.
[4] 卢轩, 靳元春, 冯宝民. 具有抗菌活性的植物内生微生物代谢产物研究进展[J]. 沈阳药科大学学报, 2015, 32(11): 892-899.
[5] Afra Khiralla, Ietidal Mohamed, Justinme Thomas, et al. A pilot study of antioxidant potential of endophytic fungi from Sudanese medicinal plants[J]. Asian Pacific Journal of Tropical Medicine, 2015, 8(9): 701-704.
[6] Rateb ME, Ebel R. Secondary metabolites of fungi from marine habitats[J]. Nat Prod Rep, 2011, 28(2): 290-344.
[7] 何佳, 刘笑洁, 赵启美, 等. 植物内生真菌分离方法的研究[J]. 食品科学, 2009, 30(15): 180-183.
[8] 张玲, 张庆波, 陈玉婵, 等. 18株南海海洋真菌的初步鉴定及其发酵产物的细胞毒活性和抗菌活性筛选[J]. 热带海洋学报, 2013, 32(3): 47-51.
[9] 马养民, 田从丽, 张弘驰. 杜仲内生真菌的分离鉴定及抗菌活性筛选[J]. 李时珍国医国药, 2011, 22(3): 552-554.
[10] Moon J K, Shibamoto T. Antioxidant assays for plant and food components[J]. Journal of Agricultural and Food Chemistry, 2009, 57(5): 1 655-1 666.
[11] 高敬红, 易大为, 邹宇, 等. 一株银杏内生真菌抗菌代谢产物的分离与鉴定[J]. 中国抗生素杂志, 2015, 40(10): 728-731.
[12] 苏印泉, 朱红薇, 马希汉, 等. 杜仲内生真菌的抑菌活性筛选[J]. 西北植物学报, 2005, 25(6): 1 153- 1 157.
[13] 金莹, 孙爱东, 胡晓丹, 等. 苹果多酚的超声波提取及其抗氧化性作用研究[J]. 北京林业大学学报, 2007, 29(5): 137-141.
[14] 王淑霞, 李爱梅, 张俊杰, 等. 龙眼核多酚物质的液质联用分析及其抗氧化活性[J]. 食品科学, 2011, 32(12): 196-203.