The enzymatic browning of pear juice was one of the major technical challenges during storage and processing. It reduced the value of pear juice such as the appearance, flavor and other quality. In this paper, crisp pear was selected as material. The inhibitory effects of enzymatic browning by browning inhibitors including ascorbic acid, calcium chloride, citric acid, β-cyclodextrin, EDTA and their combination were studied. The results showed that the order of the intensity of inhibition on the relative enzyme activity of polyphenol oxidase was ascorbic acid> calcium> citric acid> β-cyclodextrin> EDTA within the range of concentrations selected. And there was a synergistic effect among ascorbic acid, β-cyclodextrin and EDTA. The optical conditions by the orthogonal experimental design were that ascorbic acid concentration 0.10%, β-cyclodextrin concentration 0.4% and EDTA concentration 0.08%. It could not only protect polyphenol in juice, but also reduced the PPO activity to 9.6% compared with that without incorporation of anti-browning agents. Under these conditions, the pear juice product with a high color value could be obtained.

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[1] 阚建全. 食品化学[M]. 北京: 中国农业大学出版社, 2002: 251.
[2] Komthong P, Igura N, Shimoda M. Effect of ascorbic acid on the odours of cloudy apple juice[J]. Food Chemistry, 2007, 100(4): 1 342-1 349.
[3] 程双, 胡文忠, 马跃, 等. 鲜切甘薯酶促褐变调控的研究[J]. 食品工业科技, 2011, 32(6): 158-160.
[4] 赵玉生. 植酸对菠菜汁苹果汁的护色实验研究[J]. 食品研究与开发, 2000, 21(3): 19-21.
[5] 金定樑. 柠檬酸亚锡二钠对果蔬及其制品护色的研究[D]. 无锡: 江南大学, 2009.
[6] 邱龙新, 黄浩, 陈清西.半胱氨酸对马铃薯多酚氧化酶的抑制作用[J]. 食品科学, 2006, 27(4): 37-40.
[7] 程双, 胡文忠, 马跃, 等. 鲜切果蔬酶促褐变机理及控制研究进展[J]. 食品与机械, 2009, 25(4): 173-176.
[8] Sukhontha S, Kunwadee K, Chockchai T. Inhibitory effect of rice bran extracts and its phenolic compounds on polyphenol oxidase activity and browing in potato and apple puree[J]. Food Chemistry, 2015, 190: 922-927.
[9] Liu Liang, Cao Shao-qian, Yang Hua, et al. Pectin plays an important role on the kinetics properties of polyphenol oxidase from honeydew peach[J]. Food Chemistry, 2015, 168: 14-20.
[10] 宋莲军, 杨月, 唐贵芳. 复合抑制剂对苹果汁防褐变效果的影响[J]. 河南农业大学学报, 2010, 44(5): 585-590.
[11] Alvarez-Parrilla E, De La Rosa L A, Rodrigo-Garcia J, et al. Dual effect of β-cyclodextrin (β-CD) on the inhibition of apple polyphenol oxidase by 4-hexylresorcinol (HR) and methyl jasmonate (MJ) [J]. Food Chemistry, 2007, 101(4): 1 346-1 356.
[12] 赵光远, 王璋, 许时婴. 混浊苹果汁加工过程中的酶促褐变及其防止的研究[J]. 食品工业科技, 2003, 24(10): 57-61.
[13] Mishra B B, Gautam S, Sharma A. Purification and characterisation of polyphenol oxidase (PPO) from eggplant (Solanum melongena) [J]. Food Chemistry, 2012, 134(4): 1 855-1 861.
[14] 宁正祥. 食品成分分析手册[M]. 北京: 中国轻工业出版社, 1997: 306.
[15] 中华人民共和国农业部. NY/T 1600—2008 水果、蔬菜及其制品中单宁含量的测定[S]. 北京:中国农业出版社, 2008.
[16] 李健, 徐艳聪, 黄美, 等. 鸭梨果实多酚氧化酶酶学特性[J]. 食品科学, 2013, 34(15): 154-157.



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