•  
  •  
 

Abstract

The acute toxicity test, genotoxicity and subchronic oral toxicity of hemp seed oil were investigated. Results indicated that LD50 from the acute toxicity test of mouse was higher than 40 mL/kg. The negative results were showed in Ames test, micronucleus test of bone marrow and sperm abnormality test. No significant differences were found in body weight, food efficiency and blood endpoint values between testing groups and control group. Furthermore, the values of serum biochemical endpoints separately fell into the ranges of their normal specific concentrations. An absence of histological changes in rat organs was observed. It is concluded that no toxic effects of hemp seed oil were observed in this study.

Publication Date

3-28-2016

First Page

4

Last Page

11,108

DOI

10.13652/j.issn.1003-5788.2016.03.001

References

[1] 魏月媛, 李理. 火麻仁的功效及食用安全性的研究进展[J]. 食品工业, 2015, 36(7): 256-260.
[2] 扈学俸, 李永进, 王军波, 等. 火麻仁油安全性评价及血清抗氧化功能初步研究[J]. 中国食品卫生杂志, 2008, 20(5): 388-392.
[3] 中华人民共和国卫生部, 中国国家标准化管理委员会. GB 15193.3—2003 急性毒性试验[S]. 北京: 中国标准出版社, 2004.
[4] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB 15193.5—2003 骨髓细胞微核试验[S]. 北京: 中国标准化出版社, 2003.
[5] 赵淑杰, 祝寿芬, 韩光, 等. 小鼠骨髓细胞微核试验最适条件的探讨[J]. 毒理学杂志, 1993, 7(3): 178-179.
[6] 孙明, 蔡铁全. 骨髓细胞微核试验PCE/NCE比值的历史对照范围及建议[J]. 实用预防医学, 2011, 18(4): 768-769.
[7] 中华人民共和国卫生部, 中国国家标准化管理委员会. GB 15193.7—2003 小鼠精子畸形试验[S]. 北京: 中国标准化出版社, 2004.
[8] 中华人民共和国卫生部, 中国国家标准化管理委员会. GB 15193.13—2003 30天和90天喂养试验[S]. 北京: 中国标准化出版社, 2003.
[9] Krishna G, Urda G, Paulissen J. Hist orical vehicle and positive control micronucleus data in mice and rats[J]. Mutation Research/fundamental & Molecular Mechanisms of Mutagenesis, 2000, 453(1): 45-50.
[10] Aaron C S, Sorg R, Zimmer D. The mouse bone marrow micronucleus test: evaluation of 21 drug candidates[J]. Mutation Research, 1989, 223(2): 129-140.
[11] 夏义武, 程仁璋, 黄芒莉. 正交设计法研究环磷酰胺诱导小鼠髓细胞微核的影响因素[J]. 中国现代应用药学杂志, 2003, 20(2): 89-91.
[12] 罗方妮. 芝江霉素对小鼠骨髓嗜多染细胞(PCE)微核的影响[J]. 癌变畸变突变, 1996, 8(3): 183-184.
[13] Gebel T, Kevekordes S, Pav K, et al. In vivo genotocxicity of selected herbicides in the mouse bone-marrow micronucleus test[J]. Archives of Toxicology, 1997, 71(3): 193-197.
[14] 吴礼字, 吴岳文, 施纯青. 台湾金线连和阔叶大豆(金门产一条根)之初步基因毒性试验[J]. 实用预防医学, 2001, 12(3): 173-178.
[15] 考庆君, 吴坤, 李红卫. 三价铬和六价铬对小鼠骨髓细胞微核率和精子畸变的影响的比较[J]. 癌变畸变突变, 2004, 16(3): 151-154.
[16] Mcclain R, Wolz E, Davidovich A, et al. Genetic toxicity studies with genistein[J]. Food & Chemical Toxicology, 2006, 44(1): 42-55.
[17] 黄勤, 黄幸纾. 小鼠精子畸形试验在毒理学上的应用(综述)[J]. 浙江大学学报: 医学版, 1986, 15(4): 182-186.
[18] Iio K, Okada Y, Ishikura M. Single and 13-week oral toxicity study of fucoxanthin oil from microalgae in rats[J]. Journal of the Food Hygienic Society of Japan, 2011, 52(3): 183-189.
[19] Jeena K, Liju V, Kuttan R. A preliminary 13-week oral toxicity study of ginger oil in male and female Wistar rats[J]. International Journal of Toxicology, 2011, 30(6): 662-670.
[20] Kawashima H, Toyoda-Ono Y, Suwa Y, et al. Subchronic (13-week) oral toxicity study of dihomo-gamma-linolenic acid (DGLA) oil in rats[J]. Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 2009, 47(6): 1 280-1 286.
[21] Arterburn L M, Boswell K D, Koskelo E, et al. A combined subchronic (90-day) toxicity and neurotoxicity study of a single-cell source of docosahexaenoic acid triglyceride (DHASCO oil)[J]. Food & Chemical Toxicology, 2000, 38(1): 35-49.
[22] Hempenius R A, Lina B A, Haggitt R C. Evaluation of a subchronic (13-week) oral toxicity study, preceded by an in utero exposure phase, with arachidonic acid oil derived from Mortierella alpina in rats[J]. Food and Chemical Toxicology, 2000, 38(2): 127-139.
[23] 孟令仪, 宋忻恬, 张晶莹, 等. Wistar大鼠30天喂养试验体重及食物利用率参考值的研究[J]. 中国医学创新, 2014, 11(6): 84-86.
[24] 郭建强, 齐娜, 苏忆兰, 等. SPF级大鼠体重和主要脏器正常参考值的探讨[J]. 毒理学杂志, 2002, 16(4): 255-257.
[25] 王亚东, 杨海燕, 王海玉, 等. SD大鼠30 d喂养试验正常参考值的探讨[J]. 现代预防医学, 2007, 34(1): 1 265-1 267.
[26] 胡建武, 卢胜明, 车路平. 10种常见SPF级实验大、小鼠血液学及生化指标正常参考值的探讨[J]. 实验动物科学, 2007, 24(2): 5-10 .
[27] 孙劲, 徐元宏. SD大鼠常见血液学检测指标参考范围的建立[J]. 临床输血与检验, 2008, 10(3): 253-255
[28] 项华, 查捷, 孙建荣, 等. 清洁级SD大鼠血液学和血液生化学指标正常参考值的观察[J]. 中国卫生检验杂志, 2009, 19(4): 772-774.
[29] 邓雯, 陈莉莉, 刘晶, 等. SPF级SD大鼠血液学及血清生化指标正常值探讨[J]. 毒理学杂志, 2010, 24(2): 174-175.
[30] Neijat M, Gakhar N, Neufeld J, et al. Performance, egg quality, and blood plasma chemistry of laying hens fed hempseed and hempseed oil[J]. Poultry Science, 2014, 93(11): 2 827-2 840.
[31] 王亚东, 王海玉, 张聪恪, 等. SD大鼠30 d喂养试验血液学生化和脏器系数指标参考值探讨[J]. 实用预防医学, 2007, 14(4): 1 037-1 038.
[32] 夏介英, 雷培琪, 曾晓兰, 等. SPF级KM小鼠主要脏器重量和血液生化值的测定[J]. 四川生理科学杂志, 2009, 31(3): 104-107.
[33] 李慕, 王冬平, 尚士臣. 不同日龄SPF级KM小鼠血液生理生化正常指标的探讨[J]. 中国比较医学杂志, 2006, 16(9): 561.
[34] 席晓霞, 席栋宾, 吴润, 等. 3种SPF小鼠血液生理生化正常参考值的研究[J]. 黑龙江畜牧兽医, 2011, 23(12): 134-138.
[35] 李雯雯, 黄颖, 黄敏慧, 等. SPF级昆明小鼠血液生化值的测定[J]. 广东畜牧兽医科技, 2013, 38(5): 15-18.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.