Abstract
High performance liquid hromatography (HPLC) was developed to detect fructose, glucose, maltose, sucrose, glycerol, propylene glycol and sorbitol in tobacco. Samples were separated in Hi-Plex Ca cation ion exchange column with ultra pure water as mobile phase, refractive index detector as detector, and injection volume 15 μL, velocity of 0.4 mL/min, column temperature 80 ℃. The results show that the recoveries of sugars and polyols ranged from 97% to 108% with RSD of 0.89%～1.39% in the limit of detection of 1.5～2.0 mg/L. Meanwhile. Thirteen domestic and imported cigarette brands were analyzed by using this method. Results indicate that the propylene glycol, fructose, glucose, maltose and sucrose were detected in all cigarettes, while glycerol detected only in domestic cigarette No.1 and imported brand series C, this means that most cigarettes did not use glycerol as humectant. And it was found that imported brand series with two different prices use different proportions of glycerol and propylene glycol. Thus, the humectant formula of imported cigarette may give full consideration in tobacco leaf type, and it maybe worth exploring.
Publication Date
3-28-2016
First Page
73
Last Page
76
DOI
10.13652/j.issn.1003-5788.2016.03.015
Recommended Citation
Lei, GUO; Jiakun, SU; Juanmin, LUO; Da, XU; Dengyin, SHAO; Shiqing, SONG; Linghui, FAN; and Huijun, LU
(2016)
"Determination of sugars and polyols in domestic and imported cigarette brands with high performance liquid chromatography,"
Food and Machinery: Vol. 32:
Iss.
3, Article 15.
DOI: 10.13652/j.issn.1003-5788.2016.03.015
Available at:
https://www.ifoodmm.cn/journal/vol32/iss3/15
References
[1] 陈章玉, 徐若飞. 高效液相色谱法测定烟草料液中几种保润成分[J]. 理化检验: 化学分册, 2006(12): 1 049-1 051.
[2] 王岚, 李忠, 蒋次清, 等. HPLC 法同时测定烟草和料液中的糖和保润剂[J]. 烟草科技, 2006(8): 25-28.
[3] 黄菲, 黄翼飞. 高效液相色谱测定烟用香精及料液中保润剂方法的改进[J]. 现代食品科技, 2011, 27(8): 1 043-1 046.
[4] 李忠, 杨光宇, 黄海涛, 等. 高效液相色谱法测定烟草料液中的糖、甘油和丙二醇[J]. 分析化学, 2002, 30(6): 687-689.
[5] 张杰, 李鹏, 孙世豪, 等. GC/MS 法同时检测无烟气烟草制品中的1,2-丙二醇、丙三醇和三甘醇[J]. 烟草科技, 2011(3): 36-42.
[6] 王军, 高尊华, 纪朋, 等. 烟用料液及加料烟片中丙二醇的测定[J]. 安徽农学通报, 2012, 18(22): 117-118.
[7] 胡磊, 郭蓓, 陆海, 等. 植物组织中糖与糖醇乙酰化及毛细管气相色谱分析[J]. 植物学通报, 2004, 21(6): 689-699.
[8] Leffmgwell J C. Leaf chemistry: basic chemical constituents of tobacco leaf and differences among tobacco types//Dais D, Nielsen M T. Tobacco: Production Chemistry and Technology[M]. Oxford: Blackwell Science, 1999: 265-284.
[9] Talhout R, Opperhuizen A, van Amsterdam J G C. Sugars as tobacco ingredient Effects on mainstream smoke composition[J]. Food and chemical Toxicology, 2006, 44(11): 1 789-1 798.
[10] 舒俊生, 田振峰, 陈开波, 等. 烟叶中多糖的分离及单糖组成[J]. 食品与机械, 2013, 29(3): 34-36.
[11] 孙雨安, 王国庆, 张应军, 等. 高效液相色谱—蒸发光散射检测法测定烟草中水溶性糖[J]. 分析科学学报, 2004, 20(5): 531-533.