Determining the content of procyanidins from chestnut shells respectively with two kinds of methods. The average purity of purified product was 74.78% (RSD=0.99%) by using HPLC. Firstly optimizing molysite catalytic chromogenic condition of colorimetry to determine the colorimetric optimization of procyanidins from chestnut shells (CSPCs) by colorimetry with ferric ions as catalysis agent, on the basis of single-factor experiments, an orthogonal experimental design was employed, with Absorbance of extract as an index, to optimize four factors. The optimum colorimetric conditions were Fe3+ concentration of 0.01 g/mL, reaction time of 40 min, reaction temperature of 100 ℃, butanol-hydrochloric acid volume ratio (V/V) of 93∶7, the average purity of purified product was 74.67% (RSD=1.68%) by using colorimetry with ferric ions as catalysis agent. The results showed that both methods have the the advantage of good accuracy and high sensitivity, of which the HPLC method is more reliable with lower RSD. Therefore, the HPLC method can dectect the content of CSPCs purified product in a swift, accurate and practical way.

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[1] 姜贵全. 落叶松树皮原花青素的分级纯化及催化降解研究[D]. 哈尔滨: 东北林业大学, 2013: 2-10.
[2] 孙传范. 原花青素的研究进展[J]. 食品与机械, 2010, 26(4): 146-148.
[3] 董瑞霞, 李立祥, 王茜, 等. 植物中原花青素含量测定[J]. 茶业通报, 2008, 30(2): 67-69.
[4] 杜利君, 姚亚婷, 王静慧, 等. 改进铁盐催化比色法测定保健食品中原花青素[J]. 检验检疫学刊, 2013, 23(5): 50-52.
[5] 格日勒, 亓伟, 刘淑娟. 原花青素HPLC测定方法研究进展[J]. 中国酿造, 2015, 33(6): 6-9.
[6] 郑俊霞, 肖付才, 李华. 香草醛—HPLC结合法测定葡萄籽超微粉中的原花青素[J]. 食品科技, 2009, 34(11): 304-308.
[7] 张海晖, 李金凤, 段玉清, 等. 板栗壳原花青素提取及其稳定性研究[J]. 食品科学, 2011, 32(8): 5-9.
[8] 温志英, 曹妍. 响应面法优化花生红衣原花青素微波辅助提取工艺[J]. 中国粮油学报, 2011, 26(6): 97-101.
[9] 严子军, 韦寿莲, 汪洪武, 等. HPLC法测定松针中原花青素的含量[J]. 肇庆学院学报, 2012, 33(5): 26-28.

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