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Abstract

A bacteriocin-producing lactic acid bacteria strain designated as NLB-3 was isolated from natural fermentation broth of a soybean plant. By excluding the disturbances of organic acids and H2O2 to antibacterial activity of indicator bacteria, and after the fermentation broth protein stability analysis, the antibacterial substance was determined as bacteriocin. On the basis of the colonies, cells morphology, dissolved calcium circle, physiological and biochemical characteristics, strain NLB-3 was preliminary identified as Lactobacillus sp.. The biological characteristics of strain NLB-3 showed that, its optimum growth temperature was at 35 ℃, after 16 hours fermentation, the pH value reached 3.45 and it had a wider range of salt tolerance, but when the NaCl concentration was greater than 10%, and the growth of this strain was significantly inhibited.

Publication Date

3-28-2016

First Page

12

Last Page

15,64

DOI

10.13652/j.issn.1003-5788.2016.03.002

References

[1] 刘素纯, 胡茂丰, 李宗军. 自然发酵肉制品中乳酸菌的分离及特性研究[J]. 食品与机械, 2006, 22(2): 62-65.
[2] 刘书亮, 敖灵, 李燮昕, 等. 传统腌腊肉制品中乳酸菌的筛选与鉴定[J]. 食品与机械, 2007, 23(5): 14-16.
[3] 巨晓英, 韩烨, 周志江. 自然发酵泡菜中乳酸菌的分离鉴定[J]. 食品与机械, 2008, 24(5): 29-31.
[4] De Souza Barbosa M, Todorov S D, Ivanova I, et al. Improving safety of salami by application of bacteriocins produced by an autochthonous Lactobacillus curvatus isolate[J]. Food Microbiol, 2015, 46: 254-262.
[5] Barbosa M S, Todorov S D, Jurkiewicz C H, et al. Bacteriocin production by Lactobacillus curvatus MBSa2 entrapped in calcium alginate during ripening of salami for control of Listeria monocytogenes[J]. Food Control, 2015, 47: 147-153.
[6] Ghanbari M, Jami M, Kneifel W, et al. Antimicrobial activity and partial characterization of bacteriocins produced by lactobacilli isolated from Sturgeon fish[J]. Food Control, 2013, 32(2): 379-385.
[7] Hwanhlem N, Jaffrès E, Dousset X, et al. Application of a nisin Z-producing Lactococcus lactis subsp. lactis KT2W2L isolated from brackish water for biopreservation in cooked, peeled and ionized tropical shrimps during storage at 8 ℃ under modified atmosphere packaging[J]. Eur. Food. Res. Technol., 2015, 240(6): 1 259-1 269.
[8] Saraiva M A F, Nes I F, Baracat-Pereira M C, et al. Purification and characterization of a bacteriocin produced by Lactococcus lactis subsp. lactis PD6. 9[J]. Journal of Microbiology and Antimicrobials, 2014, 6(5): 79-87.
[9] Pasteris S E, Pingitore E V, Ale C E, et al. Characterization of a bacteriocin produced by Lactococcus lactis subsp. Lactis CRL 1584 isolated from a Lithobates catesbeianus hatchery[J]. World J. Microbiol Biotechnol, 2014, 30(3): 1 053-1 062.
[10] 吴清平, 黄静敏, 张菊梅, 等. 细菌素的合成与作用机制[J]. 微生物学通报, 2010, 37(10): 1 519-1 524.
[11] Desriac F, Defer D, Bourgougnon N, et al. Bacteriocin as weapons in the marine animal-associated bacteria warfare: inventory and potential applications as an aquaculture probiotic[J]. Mar Drugs, 2010, 8(4): 1 153-1 177.
[12] 陈兵, 田波, 原韬, 等. 酸菜发酵液中抑菌物质的提取与鉴定[J]. 食品与机械, 2011, 27(5): 123-125.
[13] 孔保华, 迟玉杰. Nisin在大豆制品保鲜中的应用[J]. 食品与机械, 1997(2): 28-29.
[14] 贺家亮, 秦翠丽, 康怀彬, 等. 乳酸链球菌素在食品工业中的应用[J]. 食品与机械, 2004, 20(4): 61-63.
[15] 丛建民. Nisin在草莓保鲜中的应用研究[J]. 食品与机械, 2008, 24(2): 131-133.
[16] 王凤翼, 季瑛. 乳酸菌发酵豆乳最佳条件探讨[J]. 食品与机械, 1995(4): 14-15.
[17] 于章龙, 刘楚怡, 李永歌, 等. 酸浆豆腐中菌种抗高温损伤保护剂的研究[J]. 食品科技, 2010(7): 5-8.
[18] 熊卫东, 卫军. 利用豆渣、黄浆水发酵生产曲酸的研究[J]. 食品研究与开发, 2004, 25(4): 52-57.
[19] 陈则华, 李理. 利用大豆黄浆水的发酵研究[J]. 食品研究与开发, 2007, 28(3): 42-46.
[20] 刘平, 李晓峰, 谭新敏. 利用大豆黄浆水发酵生产维生素B12的工艺探索[J]. 陕西科技大学学报: 自然科学版, 2004, 21(4): 83-85.
[21] 于海峰, 徐国华, 卢松, 等. 酸浆中嗜酸乳杆菌在豆腐废水发酵中的条件研究[J]. 发酵科技通讯, 2011, 40(1): 21-23.
[22] 刘冬梅, 李理, 杨晓泉, 等. 用牛津杯法测定益生菌的抑菌活力[J]. 食品研究与开发, 2006, 27(3): 110-111.
[23] 张小美, 楼秀玉, 顾青. 1株产细菌素乳酸菌的鉴定和细菌素的分离纯化[J]. 中国食品学报, 2013, 13(12): 181-187.
[24] 布坎南R E, 吉本斯N E. 伯杰细菌鉴定手册[M]. 中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组, 译. 北京: 科学出版社, 1984.
[25] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001.
[26] 凌代文, 东秀珠. 乳酸菌的分类鉴定及实验基础[M]. 北京: 中国轻工业出版社, 1999.

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