Abstract
16 samples were obtained from 8 sample points such as the soil of oil mill, restaurant, lampblack absorber. 9 strains were gotten by primary screening with bromocresol purple plate and secondary screening with shaking flask fermentation, and a strain, which producing lipase with 12.9 U/mL was determined original strain. By single factor and orthogonal experiment the optimum condition for high lipase-producing of the strain A7 was obtained, which was soybean oil 5 g/L, peptone 20 g/L, MgSO4·7H2O 0.4 g/L, pH 6.0, culture temperature 30 ℃, rotation speed 200 r/min. The enzyme activity of strain A7 was 19.8 U/mL under the optimum condition.
Publication Date
3-28-2016
First Page
16
Last Page
19,76
DOI
10.13652/j.issn.1003-5788.2016.03.003
Recommended Citation
Xingxiu, ZHAO; Changqing, ZHAO; Yiguo, HE; Wei, ZOU; and Jing, ZHANG
(2016)
"Study on screening of high lipase-producing strain and conditions for enzyme production,"
Food and Machinery: Vol. 32:
Iss.
3, Article 3.
DOI: 10.13652/j.issn.1003-5788.2016.03.003
Available at:
https://www.ifoodmm.cn/journal/vol32/iss3/3
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