Microwave-assisted enzyme method was adopted to improve the extraction ratio of Artemisia argyi volatile oil, the effects of enzyme extraction time, temperature, and microwave power on the extraction ratio had discussed. The extraction process was optimized with orthogonal experiment, and the ingredient of Artemisia argyi volatile oil had analyzed. The results showed that the microwave-assisted process had the benefit to get a higher extraction ratio as 60 min enzyme extraction time, 45 ℃ enzyme extraction temperature, 10 min microwave processing time, 250 W microwave power, 0.9% cellulase dosage and pH 4.0 buffer solution. The average extraction ratio was 3.61% which increased 1.25% comparing with the sample without microwave treatment. The analysis of GC—MS indicated that the main content of volatile oil was composed of ethyl benzoylformate, spathulenol and phthalic acid, which was 11.68%, 5.24% and3.94%, respectively.

Publication Date


First Page


Last Page





[1] 阳一兰, 石峰, 詹国平, 等. 艾叶挥发油的提取及其化学成分分析[J]. 安徽农业科学, 2013, 41(12): 5 267-5 271.
[2] Guan Wen-qiang, Li Shu-fen, Yan Rui-xiang, et al. Comparison of composition and antifungal activity of Artemisia argyi Lévl. et Vant inflorescence essential oil extracted by hydrodistillation and supercritical carbon dioxide[J]. Natural Product Research: Formerly Natural Product Letters, 2006, 20(11): 992-998.
[3] Hageman R H, Waygood E R. Methods for the extraction of enzymes from cereal leaves with especial reference to the triosephosphate dehydrogenases[J]. Plant Physiology, 1959, 34(4): 396-400.
[4] 张春红, 许宁, 杨悦, 等. 微波辅助法提取野生软枣猕猴桃茎黄酮的工艺优化[J]. 食品工业科技, 2011, 32(12): 335-337.
[5] Delazar A, Nahar L, Hamedeyazdan S, et al. Microwave-assisted extraction in natural products isolation[J]. Methods in Molecular Biology, 2012, 864: 89-115.
[6] Afoakwah A N, Owusu J, Adomako C, et al. Microwave assisted extraction (MAE) of antioxidant constituents in plant materials [J]. Global Journal of Bio-Science & Biotechnology, 2012, 1(2): 132-140.
[7] Viorica L A, Richard Y, Werner F B. Microwave-assisted extraction of organic compounds from standard reference soils and sediments [J]. Analytical Chemistry, 1994, 66(7): 1 097-1 106.
[8] 汪志慧, 孙智达, 谢笔钧. 响应曲面法优化双酶法提取莲房原花青素[J]. 食品科学, 2011, 32(4): 64-68.
[9] 周小楠, 董群. 正交试验优化酶法提取金银花多糖工艺[J]. 食品科学, 2012, 33(22): 119-121.
[10] Nanna R K, Marcel T A, Anne S M. Seaweed Hydrocolloid Production: An Update on enzyme assisted extraction and modification technologies[J]. Marine Drugs, 2015, 13(5): 3 340-3 359.
[11] Zuorro A, Lavecchia R. Mild enzymatic method for the extraction of lycopene from tomato paste[J]. Biotechnology & Biotechnological Equipment, 2010, 24(2): 1 854-1 857.
[12] 蔡延渠, 朱盛山, 李润萍. 新型提取联用技术在中药提取中的应用进展[J]. 中成药, 2011, 33(5): 863-866.
[13] 揭广川, 陈红杰, 李必金. 微波辅助复合酶法提取草菇中的风味物质[J]. 食品与机械, 2015, 31(1): 164-166.
[14] 陈健, 欧阳玥, 闫静. 磷酸盐缓冲溶液对邻二氮菲—Fe2+氧化法测定羟基自由基的影响[J]. 分子科学学报, 2012, 28(4): 350-352.
[15] 孙玉亮, 池建淮, 万毅. 艾叶挥发油提取工艺的研究进展[J]. 淮海医药, 2012, 30(4): 374-375.
[16] 洪宗国, 魏海胜, 张令令, 等. 不同采集期艾叶挥发油含量和化学成分的研究[J]. 中南民族大学学报: 自然科学版, 2013, 32(2): 32-35.
[17] 童红, 唐军, 张正方. 响应面法优化微波辅助水蒸气提取薰衣草挥发油的工艺研究[J]. 中国调味品, 2013, 38(10): 61-65.
[18] 李灵凤, 翟新, 金光远, 等. 真空微波破裂植物细胞壁的力学模型[J]. 食品与机械, 2014, 30(6): 18-22.
[19] 席彩彩, 张文芳, 侯明月, 等. 微波技术在植物胞内有效成分提取中的应用[J]. 中国药业, 2014, 23(3): 94-96.
[20] 孟利娜, 于敬, 许静. 酶法辅助提取北苍术挥发油的工艺及成分分析[J]. 中成药, 2013, 35(4): 844-847.
[21] Yu Xian-chun, Sun De-lin, Li Xiang-su. Preparation of furfural through the hydrolysis of rice hull using a combined biological and chemical approach[J]. Asian Journal of Chemistry, 2012, 24(4): 1 743-1 746.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.