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Abstract

GC—MS was applied to identify the acceleration of oxidative products formed from linoleic acid stored at 60 ℃. Both volatile and non-volatile oxidates were focus on in the present study. The results showed that the oxidative products formed in the primary oxidation stage were continuously oxidized into volatiles (such as aldehydes, ketones, alcohols, furan, etc.) during the course of accelerated oxidation. The relative contents of Hexanal, (E)-2-Heptena, (E)-2-Nonena, (E)-3-Nonene-2-one, 2-pentyl Furan changed obviously before 12 h and they could be used as evaluation indices of the primary autoxidation of linoleic acid. However, the relative contents of (E, E)-2, 4-Nonadienal, (E, E)-2, 4-Decadienal and (E)-1-octene-3-ol changed apparently after 24 h and they could be used as evaluation indices of secondary autoxidation of linoleic acid. Thus, the present study uncovered the potential oxidative products and possible evaluation indices of linoleic acid autooxidative at different oxidative stages, and provided a theoretical basis for concrete approach and mechanism research for autoxidation of unsaturated fatty acids.

Publication Date

5-28-2016

First Page

5

Last Page

10

DOI

10.13652/j.issn.1003-5788.2016.05.002

References

[1] Das U N. Polyunsaturated fatty acids and their metabolites in the pathobiology of schizophrenia[J]. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2013, 42(1): 122-134.
[2] Moon H S. Biological effects of conjugated linoleic acid on obesity-related cancers[J]. Chemico-Biological Interactions, 2014, 224c: 189-195.
[3] 成亮, 刘成国, 刘婷婷, 等. 共轭亚油酸的生理功能及提高其在乳制品中含量的途径[J]. 食品与机械, 2010, 26(2): 170-173.
[4] 王筱菁, 李万根, 苏杭, 等. 棕榈酸及亚油酸对人成骨肉瘤细胞MG63作用的研究[J]. 中国骨质疏松杂志, 2007, 13(8): 542-546.
[5] Frankel E N. Lipid Oxidation [M]. Second Edition. London: Woodhead Publishing, 2012: 25-50.
[6] Chen W S, Liu D C, Chen M T. The effect of roasting temperature on the formation of volatile compounds in Chinese-style pork jerky[J]. Asian Australasian Journal of Animal Sciences, 2002, 15(3): 427-431.
[7] 贝雷. 油脂化学与工艺学[M]. 徐生庚, 裘爱泳, 译. 5版. 北京: 中国轻工业出版社, 2001: 69-71.
[8] Püssa T, Raudsepp P, Toomik P, et al. A study of oxidation products of free polyunsaturated fatty acids in mechanically deboned meat[J]. Journal of Food Composition & Analysis, 2009, 22(4): 30-314.
[9] Benedetti S, Drusch S, Mannino S. Monitoring of autoxidation in LCPUFA-enriched lipid microparticles by electronic nose and SPME-GCMS[J]. Talanta, 2009, 78(4/5): 1 266-1 271.
[10] Iii R E W, Marvin R K, Hensley K, et al. Qualitative analysis of omega-3 fatty acid oxidation by desorption electrospray ionization-mass spectrometry (DESI—MS)[J]. International Journal of Mass Spectrometry, 2014, 372: 29-38.
[11] 高蓓, 章晴, 杨悠悠, 等. 固相微萃取—气质联用技术测定5种食用植物油挥发性成分[J]. 食品安全质量检测学报, 2015(7): 2 846-2 856.
[12] Lee J M, Chang P S, Lee J H. Effects of photosensitisation and autoxidation on the changes of volatile compounds and headspace oxygen in elaidic trans fatty acid and oleic cis fatty acid[J]. Food Chemistry, 2010, 119(1): 88-94.
[13] 刘冬敏. 草鱼脂质降解规律及其影响因素研究[D]. 长沙: 长沙理工大学, 2013: 22-25.
[14] 李高阳, 丁霄霖. 亚麻籽油中脂肪酸成分的GC—MS分析[J]. 食品与机械, 2005, 21(5): 30-32.
[15] 黄业传, 李凤, 黄甜, 等. 利用电子鼻和气质联用研究腊肉挥发性风味物质的形成规律[J]. 食品工业科技, 2014, 35(6): 73-80.
[16] 罗玉龙, 靳志敏, 刘夏炜, 等. 肉制品中香味物质形成原因研究进展[J]. 食品与发酵工业, 2015, 41(2): 254-257.
[17] Panseri S, Soncin S, Chiesa L M, et al. A headspace solid-phase microextraction gas-chromatographic mass-spectrometric method (HS—SPME—GC/MS) to quantify hexanal in butter during storage as marker of lipid oxidation[J]. Food Chemistry, 2011, 127(2): 886-889.
[18] García-Llatas G, Lagarda M J, Romero F, et al. A headspace solid-phase microextraction method of use in monitoring hexanal and pentane during storage: Application to liquid infant foods and powdered infant formulas[J]. Food Chemistry, 2007, 101(3): 1 078-1 086.
[19] Romeu-Nadal M, Castellote A I, López-Sabater M C. Headspace gas chromatographic method for determining volatile compounds in infant formulas[J]. Journal of Chromatography A, 2004, 1 046(1/2): 235-239.
[20] Brunton N P, Cronin D A, Monahan F J, et al. A comparison of solid-phase microextraction (SPME) fibres for measurement of hexanal and pentanal in cooked turkey[J]. Food Chemistry, 2000, 68(3): 339-345.
[21] 徐星. 植物油氧化过程中脂肪酸和挥发性成分变化的研究[D]. 杭州: 浙江工商大学, 2012: 52-58.
[22] 文志勇, 孙宝国, 梁梦兰, 等. 脂质氧化产生香味物质[J]. 中国油脂, 2004, 29(9): 41-44.
[23] 张娜, 袁信华, 过世东, 等. 中华绒螯蟹挥发性物质的研究[J]. 食品与发酵工业, 2008, 34(3): 141-144.
[24] 求海强. 食用植物油挥发性风味成分的研究[D]. 杭州: 浙江工业大学, 2009: 10-12.
[25] Karahadian C, Lindsay R C. Evaluation of compounds contributing characterizing fishy flavors in fish oils[J]. Journal of the American Oil Chemists’ Society, 1989, 66(7): 953-960.
[26] 滕迪克, 许洪高, 袁芳, 等. 脂质降解产物在肉类香气形成中的作用[J]. 中国调味品, 2008(6): 71-76.

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