A aether and n-hexane solution was used as mobile phase to preliminary extract β-sesquiphellandrene from ginger oleoresin by common silica gel column chromatography, and GC—MS was used for detecting β-sesquiphellandrene tracing. Comparison of different developing solvents of β-sesquiphellandrene and zingiberene on argentated silica gel G pre-coated plate, hexane-ethyl acetate (99∶1)was used as the mobile phase to sepatate β-sesquiphellandrene, the results indicated that the GC—MS area percentage of the silica gel and argentation column chromatography product was 95.79% with 89.73% recovery. Through the HPLC detection of β-sesquiphellandrene peak sample, β-sesquiphellandrene was found its existence of UV absorption at 232 nm, and generally had no absorption at 262 nm the characteristic absorption of zingiberene.

Publication Date


First Page


Last Page





[1] Pinder A R. 萜类化学[M]. 刘铸晋, 译. 北京: 科学出版社, 1964: 135-143.
[2] 倪明红, 陈钟瑛, 严秉淳. 光学活性姜烯类倍半萜烯的合成及其抗生育活性的探讨[J]. 华东化工学院学报, 1988, 14(6): 677-678.
[3] Vries B D. Quantitative separation of highel fatty acid methyl esters by adsorption chromatography on silica impregnated with silver nitrate[J]. JAOCS, 1963, 40: 184-186.
[4] Bennett B, Larter S R. Quantitative separation of aliphatic and aromatic hydrocarbons using silver ion-silica solid-phase extraction[J]. J. Anal. Chem., 2000, 72(5): 1 039-1 044.
[5] 欧阳长庚, 卢文彪. 利用硝酸银处理的硅胶色谱方法分离和提纯中药有效成分的思考[J]. 广州中医药大学学报, 2004, 21(4): 319-321.
[6] 王艳. 姜油树脂中姜黄烯、姜烯和β-倍半水芹烯的分离[D]. 山东: 山东大学, 2012.
[7] 贾雁高. 生姜精油中姜烯成分的分离纯化[D]. 山东: 山东大学, 2010.
[8] 朱斌, 陈晓光. 二次柱层析制备高纯度表没食子儿茶素没食子酸酯(EGCG)的工艺研究[J]. 食品与机械, 2009, 25(4): 83-85.
[9] 董新荣, 刘仲华, 何新益, 等. 辣椒色素的分离纯化与光学性质的研究[J]. 食品与机械, 2006, 22(6): 53-55.
[10] 王艳, 杜爱玲, 杜爱琴. 银离子配位柱层析法分离姜油树脂中的姜烯[J]. 精细化工, 2012, 29(7): 673-677.
[11] Ghosh A, Hoque M, Dutta. Separation of polyunsaturated fatty acid esters by argentation colurrm chromatography on silica acid[J]. J. Chromatography, 1972, 69(1): 207-208.
[12] Snyder L R, Dolan J W, Grant J R. Gradient elution in high-performance liquid chromatography[J]. Chromatography, 1979, 165(1): 3-30.
[13] Teris A, Van B, Gerrit P. Isolation and identification of the five major sesquiterpene hydrocarbons of ginger[J]. J. Phyto-chemical Analysis, 1991, 2(1): 26-34.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.