In order to optimize ultrasonic-assisted extraction of flavonoids from water caltrop pericarp, response surface methodology was used to acquire regression models for optimization of extraction temperature, extraction time and ethanol concentration based on single factor investigations. The results showed that this model reflected good relationship of the flavonoids yield and these factors. The optimal conditions for achieving the high extraction yield of flavonoids were temperature of 71.77 ℃, time of 37.96 min and ethanol concentration of 51.82%. Under the optimum conditions, the extraction yield of flavonoids reached 5.61% and fitted the models well. The obtained model could be used for extraction of total flavonoids from water caltrop pericarp.

Publication Date


First Page


Last Page





[1] 龙海荣. 菱角壳黄酮的提取、分离及抗氧化活性研究[D]. 广西: 广西大学, 2009: 1-3.
[2] 赵文亚. 菱角的营养保健功能及开发利用[J]. 农产品加工: 学刊, 2007(12): 43-44.
[3] 梁锦丽. 菱角的营养保健功能及其产品开发进展[J]. 农产品加工: 学刊, 2009(11): 78-80.
[4] 伍茶花, 丁扬洲, 裴刚, 等. 不同菱角壳提取物抑制肺癌A549细胞生长的研究[J]. 湖南中医药大学学报, 2012, 32(1): 27-30.
[5] 唐劲松, 王海蓝, 严洪清, 等. 果蜡涂膜对菱角采后品质和生理的影响[J]. 食品与机械, 2014, 30(3): 142-148.
[6] Lin Qiu-sheng, Yu Hai-ning, Das U N, et al. Water caltrop pericarps extracts attenuate H2O2-induced Human umbilical vein endothelial cell injury[J]. Advanced Materials Research, 2012, 554-556: 1 757-1 761.
[7] Kim Y S, Hwang J W, Han Y K, et al. Antioxidant activity and protective effects of Trapa japonica pericarp extracts against tert-butylhydroperoxide-induced oxidative damage in Chang cells[J]. Food and Chemical Toxicology, 2014, 64(2): 49-56.
[8] Yasuda M, Yasutake K, Hino M, et al. Inhibitory effects of polyphenols from water chestnut ( Trapa japonica ) husk on glycolytic enzymes and postprandial blood glucose elevation in mice[J]. Food Chemistry, 2014, 165(20): 42-49.
[9] Lin Qiu-sheng, Shen Jun-hui, Ning Ying, et al. Inhibitory effects of water caltrop pericarps on the growth of human gastric cancer cells in vitro[J]. Current Pharmaceutical Design, 2013, 19(42): 7 473-7 478.
[10] 李鹏婧, 柳旭光, 龙海荣, 等. 超声波辅助提取菱角壳总黄酮及抗氧化性研究[J]. 食品科技, 36(1): 167-171.
[11] 李维莉, 马银海, 张亚平, 等. 菱角壳总黄酮超声辅助提取工艺研究[J]. 食品科学, 2009, 30(14): 140-142.
[12] 宋棋芬, 高广春, 曹琳茸, 等. 南湖菱壳总黄酮的水提醇沉工艺[J]. 浙江农业科学, 2013, 1(8): 1 029-1 031.
[13] 侯莉莉, 徐燕燕, 吴佳琴, 等. 响应面法优化菱角壳总黄酮的提取工艺[J]. 农产品加工, 2015(8): 25-28.
[14] 柳旭光. 青钱柳黄酮的提取分离、抗氧化活性及其应用研究[D]. 广西: 广西大学, 2012: 92-93.
[15] 罗丽, 梁琪, 张炎, 等. 响应面法优化超声波辅助提取沙枣果总黄酮工艺[J]. 食品工业科技, 2013, 34(5): 269-274.
[16] Wang Shi-hao, Kao Ming-yuan, Wu She-ching, et al. Oral administration of Trapa taiwanensis Nakai fruit skin extracts conferring hepatoprotection from CCl4-caused injury[J]. Journal of Agricultural & Food Chemistry, 2011, 59(8): 3 686-3 692.
[17] Tien-tso W U, Shiau S Y, Chang Rei-chu, et al. Effect of drying temperature and extraction solvent on the antioxidant, color and sensory properties of Angelica Sinensis leaves[J]. Journal of Food Processing and Preservation, 2013, 37(4): 371-379.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.