Effects of sodium citrate were investigated on the growth, the serum biochemical parameters and intestinal flora of mice. Mice were randomly divided into four groups: control group, low-dose group (added 0.65% sodium citrate), middle-dose group (added 2.6% sodium citrate) and high-dose group (added 5.2% sodium citrate).The diet intervention lasted for four weeks. Stool samples and weight data were collected weekly, and the composition of intestinal microbiota and the serum biochemical parameters were determined at the end of the fourth week. Results showed that the addition of sodium citrate made the body weight of mice less than control group. And the high-dose group’s high density lipoprotein cholesterol (HDL-C) had a significantly increased. The beta diversity and the ratio of Fimicutes to Bacteriodetes were changed by intake of sodium citrate. At the genus level, Corynebacterium and Staphylococcus were detected in the high-dose group. Results indicated that the intestinal flora of mice could be affected by intake of high dose of sodium citrate.

Publication Date


First Page


Last Page





[1] Savage D C. Microbial ecology of the gastrointestinal tract[J]. Annual Review of Microbiology, 1997, 31: 107-133.
[2] Bckhed F, Ruth E Ley, Justin L, et al. Host-Bacterial mutualism in the human intestine[J]. Science, 2005, 307(5 717): 1 915-1 920.
[3] Sommer F, Bckhed F. The gut microbiota - masters of host development and physiology[J]. Nature Reviews Microbiology, 2013, 11(4): 227-238.
[4] 赵兰涛, 任玮, 张英, 等. 黑米对小鼠肠道菌群的影响[J]. 食品与机械, 2012, 28(3): 24-27.
[5] Carmody R N, Gerber G K, Luevano J M, et al. Diet dominates host genotype in shaping the murine gut microbiota[J]. Cell Host & Microbe, 2015, 17(1): 72-84.
[6] David L A, Maurice C F, Carmody R N, et al. Diet rapidly and reproducibly alters the human gut microbiome[J]. Nature, 2014, 505(7 484): 559-563.
[7] 杨金席. 柠檬酸钠的应用与生产工艺[J]. 辽宁化工, 2002, 31(6): 212.
[8] 陈魏勇. 冰棒、冰淇淋添加剂复配研究与应用[D]. 湖南: 湖南农业大学, 2008: 24-33.
[9] Ghanaatparast Rashti M, Shariatmadari F, Karimi Torshizi, et al. Effects of dietary propionic acid, sodium citrate, and phytase on growth performance, mineral digestibility, and tibia properties in broilers[J]. Journal of Applied Animal Research, 2016, 44(1): 370-375.
[10] 黄继汉, 黄晓晖, 陈志扬, 等. 药理试验中动物间和人体间的等效剂量换算[J]. 中国临床药理学与治疗学, 2004, 9(9): 1 069-1 072.
[11] Zhao Le-le, Wang Gang, Siegel P, et al. Quantitative genetic background of the host influences gut microbiomes in chickens[J]. Scientific Reports, 2013, 3: 1 163.
[12] Maughan H, Wang P W, Diaz Caballero J, et al. Analysis of the cystic fibrosis lung microbiota via serial illumina sequencing of bacterial 16S rRNA hypervariable regions[J]. PLoS ONE, 2012, 7(10): e45 791.
[13] Lozupone C, Knight R. UniFrac: a new phylogenetic method for comparing microbial communities[J]. Applied and Environment Microbiology, 2005, 71: 8 228-8 235.
[14] Huson D H, Mitra S, Weber N, et al. Integrative analysis of environmental sequences using MEGAN4[J]. Genome Research, 2011, 21: 1 552-1 560.
[15] Rader D J. High-density lipoproteins and atherosclerosis[J]. The American Journal of Cardiology, 2002, 90(8): 62-70.
[16] Nishina P M, freedland R A. Effects of propionate on lipid biosynthesis in isolated rat hepatocytes[J]. The Journal of Nutrition, 1990, 120(7): 668-673.
[17] Hamady M, Knight R. Microbial community profiling for human microbiome projects: Tools, techniques, and challenges[J]. Genome Research, 2009, 19(7): 1 141-1 152.
[18] Kemp P F, Aller J Y. Bacterial diversity in aquatic and other environments: what 16S rDNA libraries can tell us[J]. FEMS Microbiology Ecology, 2004, 47(2): 161-177.
[19] Hamady M, Lozupone C, Knight R. Fast UniFrac: facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and PhyloChip data[J]. ISME J., 2010, 4(1): 17-27.
[20] Eckburg P B, Bik E M, Bernstein C N, et al. Diversity of the human intestinal microbial flora[J]. Science, 2005, 308(5 728): 1 635-1 638.
[21] Ley Ruth E, Bckhed Fredrik, Turnbaugh Peter, et al. Obesity alters gut microbial ecology[J]. Proceedings of the National Academy of Sciences, 2005, 102(31): 11 070-11 075.
[22] Murray P R. Manual of clinical microbiology[M]. Washington D C: American Society for Microbiology Publication, 1995: 235.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.