Abstract
Fresh plants of T. chinensis collected from Inner Mongolia were used to isolated the endophytic fungi from their roots, stems and leaves after superficial disinfection. According to the morphological characteristics observation and microscopy examination, 6 endophytic fungi strains were isolated from the T. chinensis, including four strains from the leaves, one from the stems, and one from the roots. All the separated fungi strains were identified as penicillium genus (Pinicielium).
Publication Date
6-28-2016
First Page
41
Last Page
43
DOI
10.13652/j.issn.1003-5788.2016.06.010
Recommended Citation
Jun, LU; Peizhen, QIN; Qiji, SHAN; and Zhonghai, LI
(2016)
"Study on isolation and preliminary identification of endophytic fungi in Trollius chinensis,"
Food and Machinery: Vol. 32:
Iss.
6, Article 10.
DOI: 10.13652/j.issn.1003-5788.2016.06.010
Available at:
https://www.ifoodmm.cn/journal/vol32/iss6/10
References
[1] 王莉衡. 植物内生菌的研究进展[J]. 化学与生物工程, 2011, 28(3): 5-8.
[2] 官珊, 钟国华, 孙之潭, 等. 植物内生真菌的研究进展[J]. 仲恺农业技术学院学报, 2005, 18(1): 61-66.
[3] 史长东. 植物内生菌活性代谢产物的研究进展[J]. 现代园艺, 2010(21): 13-13.
[4] 肖淑贤. 植物内生菌的研究概况及应用进展[J]. 农业技术与装备, 2011(2): 74-77.
[5] 赵宇, 刘浩舶, 李振, 等. 海带内生菌HSN2胞外蛋白对粉红单端孢霉的影响[J]. 食品与机械, 2014, 30(6): 31-34.
[6] 贾栗, 陈疏影, 翟永功, 等. 近年国内外植物内生菌产生物活性物质的研究进展[J]. 中草药, 2007, 38(11): 1 751-1 754.
[7] 赵凯, 平文祥, 周东坡. 内生真菌发酵生产紫杉醇的研究现状与展望[J]. 微生物学报, 2008, 48(3): 403-407.
[8] BHATIA D R, DHAR P, MUTALIK V, et al. Anticancer activity of Ophiobolin A, isolated from the endophytic fungus Bipolaris setariae[J]. Natural Product Research, 2016, 30(12): 1 455-1 458.
[9] LIN Zhen-jian, ZHU Tian-jiao, FANG Yu-chun, et al. Polyketides from Penicillium sp·JP-1, an endophytic fungus associated with the man-grove plant Aegiceras corniculatum[J]. Phytochemistry, 2008, 69(5): 1 273-1 278.
[10] WANG Xiao-peng, ZHONG Jia, WANG Xing-hong et al. Study on the bioactivity of the endophytic fungi isolated from SinoPo Dophyllumh exandum and Diphylleia sinensis[J]. Nat. Prod. Res. Dev., 2006, 18(1): 152-191.
[11] STROBEL G, DAISY B, CASTILLO U, et al. Natural products from endophytic microorganisms [J]. Journal of Natural Products, 2004, 67(2): 257-268.
[12] 郑丹, 刘占华, 张剑, 等. 4株抗肿瘤活性物质喜树内生真菌的鉴定及其主要代谢产物HPLC初步比较[J]. 药物生物技术, 2007, 14(3): 190-195.
[13] 侯奎, 刘玉军, 陆荣, 等. 葛内生真菌次生代谢产物清除自由基活性的研究[J]. 安徽农业大学学报, 2008, 35(2): 262-265.
[14] 刘霞, 党峰峰. 陕北野生甘草内生菌的分离及抑菌活性筛选[J]. 西北植物学报, 2010(10): 2 110-2 115.
[15] BHIMBA B V, FRANCO D A A D, MATHEW J M, et al. Anticancer and antimicrobial activity of mangrove derived fungi Hypocrea lixii, VB1[J]. Chinese Journal of Natural Medicines, 2012, 10(1): 77-80.
[16] 李青连, 苏红, 池维丹, 等. 野生黄芩内生真菌的分离鉴定及抗菌活性筛选[J]. 微生物学通报, 2010(3): 381-388.
[17] 江军山, 张鑫. 产抗菌活性物质植物内生菌的研究进展[J]. 安徽农业科学, 2011, 38(22): 1 704-1 705.
[18] 袁勤洋. 中药金莲花药理作用研究进展[J]. 人民军医, 2011, 54(9): 825-826.
[19] 吴新安, 秦峰, 都模勤. 短瓣金莲花挥发油成分的气相色谱—质谱联用分析[J]. 解放军药学学报, 2011, 27(6): 519-520
[20] 魏金霞, 李丹毅. 金莲花化学成分的分离与鉴定(Ⅱ)[J]. 沈阳药科大学学报, 2012, 29(1): 12-15.
[21] 王书华, 杨国栋, 饶娜, 等. 金莲花中荭草苷和牡荆苷对D-半乳糖致衰小鼠体内抗氧化作用的影响[J]. 中国老年学杂志, 2011, 31(24): 4 818-4 820.
[22] 杨国栋, 饶娜, 田嘉铭, 等. 金莲花中荭草苷和牡荆苷抗氧作用的研究[J]. 时珍国医国药, 2011, 22(9): 2 172-2 173.
[23] LI Hai-ping, ZHANG Ming-ming, MA Gui-ji. Radical scavenging activity of flavonoids from Trollius chinensis bunge [J]. Nutrition, 2011, 27(10): 1 061-1 065.
[24] 刘金花, 吴玲芳, 章华伟. 黄花蒿内生菌的分离与初步鉴定[J]. 氨基酸和生物资源, 2011, 33(4): 27-30.
[25] 杨国栋. 瑞香狼毒内生真菌对原植物成分降解作用的研究[D]. 西安: 西北农林科技大学, 2009: 30-32.
[26] 刘建玲, 陈宝宝, 刘永红, 等. 半夏内生菌的分离与初步鉴定[J]. 中国中药杂志, 2009, 34(18): 2 305-2 307.
[27] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979: 133-134.