Enriched the drinking water by using tangential flow ultrafiltration (TFF), extracted the genomic DNA, amplified 16S rDNA V4+V5 region, a total of 45 093 sequences and the average length of the 395.65 bp sequence were got. After high throughput sequencing bioinformatics analysis including annotation, evaluation, species composition analysis, β- diversity analysis, species difference analysis, RDA analysis etc., the bacterial diversity and the abundance in drinking water was got. In the result, the bacterial diversity is uncertainty and randomness in drinking water, the water quality parameters of turbidity and residual chlorine are biggest influence on the bacterial diversity in drinking water, but the bacteria bio-information which got by Illumina MiSeq high-throughput sequencing may exist without activity or ‘viable but non-culturable bacteria’, but these bacteria are difficult to culture. High-throughput sequencing technology is a good technology for analyzing the bacteria in drinking water.
Yingliang, GE; Shuili, YU; Wenxin, SHI; Qi, YIN; and Weipeng, WENG
"Analysis bacterial diversity in drinking water of O3-BAC water treatment technology by Illumina MiSeq sequencing,"
Food and Machinery: Vol. 32:
6, Article 13.
Available at: https://www.ifoodmm.cn/journal/vol32/iss6/13
 MONTGOMERY M A, ELIMELECH M. Water and sanitation in developing countries: Including health in the equation[J]. Environmental Science & Technology, 2007, 41(1): 17-24.
 张良荣, 倪欣. 臭氧生物活性炭深度处理工艺在吴江第二水厂的应用[J]. 辽宁化工, 2015(7): 908-910.
 林瑜. 浅谈臭氧—生物活性炭深度水处理工艺[J]. 科技与创新, 2014(4): 156-161.
 QIAO Tie-jun, ZHANG X, WU Guang-xue, et al. Investigation of microbial safety of a full-scale ozonation and biological activated carbon process under high humidity and temperature conditions[J]. Water Science & Technology, 2011, 64(11): 2 293.
 祝泽兵, 吴晨光, 钟丹, 等. 寒区湖库型水源供水系统中耐氯菌的生长[J]. 哈尔滨工业大学学报, 2015(8): 1-6.
 ZMIROU D, PENA L, LEDRANS M, et al. Risks associated with the microbiological quality of bodies of fresh and marine water used for recreational purposes: summary estimates based on published epidemiological studies[J]. Arch. Environ. Health, 2003, 58(11): 703-711.
 LUND V. Evaluation of E-coli as an indicator for the presence of Campylobacter jejuni and Yersinia enterocolitica in chlorinated and untreated oligotrophic lake water[J]. Water research, 1996, 30(6): 1 528-1 534.
 HARWOOD V J, STALEY C, BADGLEY B D, et al. Microbial source tracking markers for detection of fecal contamination in environmental waters: relationships between pathogens and human health outcomes[J]. FEMS Microbiol. Rev., 2014, 38(1): 1-40.
 BONADONNA L, BRIANCESCO R, OTTAVIANT M, et al. Occurrence of Cryptosporidium oocysts in sewage effluents and correlation with microbial, chemical and physical water variables[J]. Environ. Monit. Assess, 2002, 75(3): 241-252.
 RAMAMURTHY T, GHOSH A, PAZHANI G P, et al. Current perspectives on viable but non-culturable (VBNC) pathogenic bacteria[J]. Frontiers in Public Health, 2014, 2(4): 1-9.
 SHAW J L A, MONIS P, WEYRICH L S, et al. Using amplicon sequencing to characterize and monitor bacterial diversity in drinking water distribution systems[J]. Applied and Environmental Microbiology, 2015, 81(18): 6 463-6 473.
 AW T G, ROSE J B. Detection of pathogens in water: from phylochips to qPCR to pyrosequencing[J]. Curr. Opin. Biotechnol., 2012, 23(3): 422-430.
 MARTIN-LATIL S, HENNECHART-COLLETTE C, GUILLIER L, et al. Duplex RT-qPCR for the detection of hepatitis E virus in water, using a process control[J]. International Journal of Food Microbiology, 2012, 157(2): 167-173.
 VESPER S J, HAUGLAND R A, ROGERS M E, et al. Opportunistic Aspergillus pathogens measured in home and hospital tap water by quantitative PCR (QPCR)[J]. Journal of Water and Health, 2007, 5(3): 427.
 URSELL L K, GUNAWARDANA M, CHANG S, et al. Comparison of the vaginal microbial communities in women with recurrent genital HSV receiving acyclovir intravaginal rings[J]. Antiviral Research, 2014(102): 87-94.
 DINGSDAG S, COLEMAN N V. Bacterial communities on food court tables and cleaning equipment in a shopping mall[J]. Epidemiology and Infection, 2013, 141(8): 1 647-1 651.
 METHE B A, NELSON K E, POP M, et al. A framework for human microbiome research[J]. Nature, 2012, 486(7 402): 215-221.
 PANDEY R V, NOLTE V, SCHLOTTERER C. CANGS: a user-friendly utility for processing and analyzing 454 GS-FLX data in biodiversity studies[J]. BMC Res Notes, 2010(3): 3.
 TAN B, NG C, NSHIMYIMANA J P, et al. Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities[J]. Frontiers in Microbiology, 2015(21): 7 626-7 637.