Study on mutagenic and anti-mutafenic effect of Korean soybean paste with different storage period
In order to explore the mutagenic and anti-mutagenic effect on the different storage periods of Korean soybean paste. In this study, on the basis of detecting mutagenicity effect by modified Ames test, and detecting the anti-mutagenic effects of the Korean soybean paste samples with different storage time have on Mouse bone marrow micronucleus test. Result: the mutagenicity of Korean soybean paste were negative; Anti-mutagenic effect were enhanced with the extension of storage time and the concentration, the anti-mutagenic effect of TA100 and TA98 strains induced by Trp-p-1 and MNNG reached the maximum value at 12 months of storage. And there was a dose-concentration-response relationship. At the same time, there was significant inhibitory effect of Korean soybean paste extracts with storage 12 months on mouse bone marrow micronucleus, the highest inhibition rate was up to 46.5% in the test. The results showed the strongest anti-mutagenic effect of Korean soybean paste was which stored for 12 months and no mutagenicity found, provided an experimental proof for the further development of Korean soybean paste in nutritional, functional, and for basic research and the promotion of cancer diet.
Xue, LI; Dejun, SHI; Baishen, HUANG; Bin, XU; and Chengbi, CUI
"Study on mutagenic and anti-mutafenic effect of Korean soybean paste with different storage period,"
Food and Machinery: Vol. 32:
6, Article 29.
Available at: https://www.ifoodmm.cn/journal/vol32/iss6/29
 杜振亚, 陈复生. 大豆蛋白保健功能研究进展[J]. 食品与机械, 2014, 30(6): 247-250.
 杨金萍. “芜荑盐豉醯酢酱”的食用与药用[J]. 中华医史杂志, 2010, 40(2): 72-76.
 PARK K Y, JUNG K O, RHEE S H, et al. Antimutagenic effects of doenjang (Korean fermented soypaste) and its active compounds[J]. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2003, 523-524: 43-53.
 HUNG Yu-Hsiang, WANG Yen-Ju, CHOU Cheng-Chun. Antimutagenic activity of aspergillus awamori-fermented black soybean response to simulated digestive juice treatments and its antimutagenic mechanisms[J]. LWT - Food Science and Technology, 2009, 42(1): 56-62.
 陈湘宁, 郭琳博, 李宇华, 等. 酒精性肝病发病机制及防治机理研究[J]. 食品与机械, 2011, 27(6): 265-268.
 SHEIH I-Chuan, TONY J Fang, WU Tung-Kung, et al. Effects of fermentation on antioxidant properties and phytochemical composition of soy germ[J]. Journal of The Science of Food and Agriculture, 2014, 94(15): 3 163-3 170.
 HAM Seung-Shi, KIM Soo-Hyun, MOON Sun-Young, et al. Antinuiragenic effects of subfractions of Chaga mushroom (Inonotus obliquus) extract[J]. Mutation Research, 2009, 672(1): 55-59.
 DOROTHY M Maron, BRUCE N Ames. Revised methods for the salmonella mutagenicity test[J]. Mutation Research, 1983, 113(3/4): 173-215.
 HAM S S, OH S W, KIM Y K, et al. Antimutagenic and cytotoxic effects of ethanol extract from the Inonolus obliquus[J]. J. Korean Soc. Food Sci. Nutr., 2003, 32: 1 088-1 094.
 CUI Cheng-Bi, LEE E Y, HAM S S, et al. Antimutagenic and cytotoxic effects of an ethanoi extract of buckwheat sprout[J]. J. Korean Soc. Appl. Biol. Chem., 2008, 51(3): 212-218.
 THOMSON I S I, ASMA Saeed, BUSHRA Sultana, et al. Alkharfy and anwarul-hassan gilani[J]. International Journal of Pharmacology, 2014, 10(8): 461-469.
 GANESH Chandra, JAGETIA T K R. The grapefruit fiavanone naringin protects against the radiation-induced genomic instability in the mice bone marrow: a micronucleus study[J]. Mutation Research, 2002, 519(2): 37-48.
 BOWEN D E, WHITWELL J H, LILLFORD L, et al. Evaluation of a multi-endpoint assay in rats, combining the bone-marrow micronucleus test, the comet assay and the flow-cytometric peripheral blood micronucleus test[J]. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2011, 722(1): 7-19.
 方选, 李昇刚. 西洋参胶囊对小鼠骨髓细胞染色体和微核的影响[J]. 中国现代应用药学, 2012(9): 786-788.
 卿艳, 郭亚杰, 陈锦瑶, 等. 体内外微核试验评价亚硒酸钠的致突变性和抗突变性[J]. 预防医学情报杂志, 2013(3): 234-237.
 赵欣, 冉龙江, 邵林楠. 水豆豉和纳豆的理化特性及抗突变效果的比较[J]. 淮坊教育学院学报, 2008, 21(2): 49-50.
 韩弛, 徐勇, 施瑞丽, 等. 中国传统加工食品致突变性的研究[J]. 癌变·畸变·突变, 1991(1): 8-12.