Extraction and purification of polysaccharide from spirulina and its anti-colonic adenocarcinoma capacity
The polysaccharide was from Spirulina powder by the means of hot water extraction after repeated freezing-thawing cycles and ultrasonic treatment. The effects of lasted time and extraction temperature and time, ratio of solid to liquid and times of extraction on polysaccharide yield were studied by single factor and orthogonal experiments. PSP was separated into PSP-3 by membrane separation techniques, and it was investigated by MTT assay on colon adenocarcinoma cells (Caco-2 cell growth inhibition) to determine the optimal concentration. Finally, the molecular of PSP-3 was determined by gel permeation chromatography (GPC). Experiment results showed that the optimum conditions were as follows: ultrasonic treating time 20 min, extracting temperature 90 ℃, ratio of material to liquid 1∶25, extracting time 1.5 hours, extracting time twice. Under the conditions, the yield reached (13.17 ± 0.49)%, being 1.59 times of the polysaccharides prepared by traditional water extraction. PSP was separated and purified into PSP-3 and its inhibition on colon adenocarcinoma cells (Caco-2 cells) was investigated. The results indicated that the molecular weight of PSP-3 was 623.02 kDa and the optimum inhibitory concentration was 5.00 mg/mL, with IC50 value of 0. 63 mg/mL.
Qun, WANG; Shike, CHEN; Haoyao, LI; Xuexiang, CHEN; and Yong, CAO
"Extraction and purification of polysaccharide from spirulina and its anti-colonic adenocarcinoma capacity,"
Food and Machinery: Vol. 32:
6, Article 36.
Available at: https://www.ifoodmm.cn/journal/vol32/iss6/36
 LE T, KNULST A C, Roeckmann H. Anaphylaxis to Spirulina confirmed by skin prick test with ingredients of Spirulina tablets[J]. Food and Chemical Toxicology, 2014, 74: 309-310.
 PELIZER L H, CARVALHO J C M, MORAES I. Protein production by Arthrospira (Spirulina) platensis in solid state cultivation using sugarcane bagasse as support[J]. Biotechnology Reports, 2015(5): 70-76.
 DYER A P, BANFLELE B W, MARTINDALE D, et al. Dextran sulfate can act as an artificial receptor to mediate a typespecific herpes simplex virus infection via glycoprotein B[J]. Journal of Virology, 1993, 71(1): 191-198
 游丽君, 邹林武, 梁彦豪, 等. 超声—高温热水提取香菇多糖及其产物特性研究[J]. 现代食品科技, 2013, 29(9): 2 167-2 172.
 贲永光, 钟红茂, 李康, 等. 超声辅助提取螺旋藻多糖的实验研究[J]. 中成药, 2011, 33(6): 1 078-1 080.
 亓树艳, 王荔, 莫晓燕.大枣多糖的提取工艺及抗氧化作用研究[J].食品与机械, 2012, 28(4): 117-120.
 史红艳, 余静丹, 王丹, 等. 超滤过结合密度梯度离心纯化鲍曼不动杆菌噬菌体方法的建立[J]. 中国微生态学杂志, 2013, 25(3): 350-352.
 隋璐, 刘坤, 沈薇, 等. 螺旋藻多糖对胃溃疡大鼠胃液分泌和前列腺素E_2的影响[J]. 中国医药导报, 2013, 10(25): 7-9.
 隋璐, 刘坤, 沈薇, 等. 螺旋藻多糖对胃溃疡大鼠胃黏膜的修复作用及对EGF和EGFR表达的影响[J]. 国实验方剂学杂志, 2013, 19(20): 162-165.
 HIDALGO I J, RAUB T J, BORCHARDT R T. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability, gastroenterology, 96, 736-749, 1989-the backstory[J]. Aaps Journal, 2011, 13(3): 323-327.
 IRAHA A, CHINEN H, HOKAMA A, et al. Fucoidan enhances intestinal barrier function by upregulating the expression of claudin-1[J]. World Journal of Gastroenterology, 2013, 19(33): 5 500-5 507.
 穆文静, 杜玲, 扈瑞平, 等. 钝顶螺旋藻多糖Sevage法脱蛋白工艺的研究[J]. 内蒙古石油化工, 2011(10): 1-4.
 YASUO I, TORU T, KYUICHI Y, et al. Control of viscosity in starch and polysaccharide solutions with ultrasound after gelatization[J]. Food Sci. Emerg Technol., 2008(9): 140-146.
 金鑫, 赖凤英. 仙人掌多糖的提取、分离纯化及GPC法测定其分子量[J]. 现代食品科技, 2006, 88(2): 138-140.
 冯怡, 薛明, 姜玲海, 等. 夏枯草中水溶性多糖的分离纯化及化学研究[J]. 中国医院药学杂志, 2008, 28(6): 431-434.
 FERRARI M, FORNASIERO M C, ISETTA A M. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro [J]. Journal of immunological methods, 1990, 131(2): 165-172.
 周岩冰, 于红, 赵磊. 螺旋藻多糖对体外培养胃肠道癌细胞的抑制作用[J]. 中华实验外科杂志, 2004, 21(5): 537.
 CHAIKLAHAN R, CHIRASUWAN N, TRIRATANA P, et al. Polysaccharide extraction from Spirulina sp and its antioxidant capacity[J]. International Journal of Biological Macromolecules, 2013, 58: 73-78.