Abstract
In order to find an efficient and systematic way of producing and purifying geraniol glycosidase，an improved method of Koenigs-Knorr was gotten, which used the silicone to hold silver carbonate catalyst to improve the productivity and perfects the synthesis process, separating and purifying the crude sample by silica gel column chromatography(SGCC) and high performance liquid chromatography (HPLC) . The method of SGCC applies the thin plate chromatography to get the better flow matches which is the ethyl acetate and ethanol according to 50:1, 40:1, 30:1, but the HPLC selects water and acetonitrile which is to 45:55, each of the two flows with the raw sample. The results showed that the new way of synthsis could improve product yield from 61% to 68% and simplify the synthesis process; The purity of the final product purified by silica gel was 90.12%, and the purity of the final product purified by HPLC was 98.66%, both of them could get the better purity of geraniol glycoside. It provides a reference for the synthesis and purification of geraniol glycosidase.
Publication Date
6-28-2016
First Page
171
Last Page
175
DOI
10.13652/j.issn.1003-5788.2016.06.041
Recommended Citation
Dongwei, CHENG; Xihong, YANG; Wancui, XIE; Qian, GAO; and Sheng, LEI
(2016)
"Separation and purification geraniol-beta-D-glycosidase by chemical synthesis,"
Food and Machinery: Vol. 32:
Iss.
6, Article 41.
DOI: 10.13652/j.issn.1003-5788.2016.06.041
Available at:
https://www.ifoodmm.cn/journal/vol32/iss6/41
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