Multi-stage cyclone grinding can be used to change the physical structure of corn straw, which can be degraded easier by enzymes to release feruloylated saccharides. By the same enzymatic hydrolysis, the content of feruloylated saccharides released from multi-stage cyclone grinding corn straw increased by 3.34 fold than that without pretreatment. Commercial complex enzymes, such as Validase TRL, HSP 6000 BG, Rapidase Smart Clear, xylosidase and arabinfuranosidease were used to hydrolyze multi-stage cyclone grinding corn straw, and it showed that Validase TRL had the best effect, and the yield of feruloylated saccharides was 1.46 μmol/g, increased by 3.48 fold than that without hydrolysis. The best combination of synergy enzymatic hydrolysis was Validase TRL 100 U/g, xylosidase 50 U/g and arabinfuranosidease 50 U/g, and the highest yield of feruloylated saccharides was 2.87 μmol/g. After the hydrolysater was pured by macroreticular resin, the recovery of feruloylated saccharides reached 81.44%.

Publication Date


First Page


Last Page





[1] 徐斌. 电化学预处理玉米秸秆的研究[D]. 北京: 中国地质大学, 2014.
[2] 孙万里, 陶文沂. 木质素与半纤维素对稻草秸秆酶解的影响[J]. 食品与生物技术学报, 2010, 29(1): 18-22.
[3] MATHWE S, ABRAHAM T E. Ferulic acid: an antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their application[J]. Critical Review in Biotechnology, 2004, 24(2/3): 59-83.
[4] 姚艳艳. 小麦麸皮阿魏酸提取与纯化工艺研究[D]. 雅安: 四川农业大学, 2011.
[5] 欧仕益. 阿魏酸的功能和应用[J]. 现代食品科技, 2002, 18(4): 50-53.
[6] 宋凯, 单安山. 阿魏酸的生理作用及其在饲料中的应用前景[J]. 饲料博览, 2004, 16(9): 44-46.
[7] 许仁, 溥许大. 阿魏酸应用开发[J]. 粮食与油脂, 2000, 13(6): 7-9.
[8] 曾凤彩. 酶解玉米麸皮制备阿魏酰低聚糖及抗氧化性质的研究[D]. 齐齐哈尔: 齐齐哈尔大学, 2012: 6.
[9] 林奇龄, 温其标, 欧仕益, 等. 酸解制备低聚糖阿魏酸酯的优化工艺研究[J]. 食品研究与开发, 2012, 33(6): 81-85.
[10] 姚惠源, 胡敏, 袁小平, 等. 酶法制备阿魏酰低聚糖的研究[J]. 食品与机械, 2008, 24(4): 3-7.
[11] RACHEL R S, ANDREAS B, CATRIN E T, et al. Isolation and characterization of feruloylated arabinoxylan oligosaccharides from the perennial cereal grain intermediate wheat grass (Thinopyrum intermedium)[J]. Carbohydrate Research, 2015, 407: 16-25.
[12] NOBUAKI S, YOHEI T, MASAHIRO M, et al. Production of feruloylated arabino-oligosaccharides(FA-AOs) from beet fiber by hydrothermal treatment[J]. The Journal of Supercritical Fluides, 2013, 79: 84-91.
[13] 王璐璐, 蔡国林, 朱德伟, 等. 球磨预处理和固态发酵对玉米秸秆饲用品质的影响[J]. 食品与生物技术学报, 2014(11): 1 148-1 153.
[14] 刘元元, 徐泽平. 阿魏酸酯酶酶活性的分光光度法测定及影响因素的研究[J]. 食品工业科技, 2013(13): 284-288.
[15] FERREIRA P, DIEZ N, FAULDS C B, et al. Release of ferulic acid and feruloylated oligosaccharides from sugar beet pulp by Streptomyces tendae[J]. Bioresource Technology, 2007, 98(8): 1 522-1 528.
[16] 张麟, 蔡国林, 高献礼, 等. 粉碎及酶处理对啤酒糟理化特性的影响[J]. 食品与发酵工业, 2013, 39(12): 13-18.
[17] NIETER A, HAASE A P, LINKE D, et al. A halotolerant type A feruloyl esterase from Pleurotus eryngii[J]. Fungal Biology, 2014, 118(3): 348-357.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.