Effects of lipase activity regulation in ionic liquids on synthesis of starch stearate were investigated in the present study. The results showed that degree of substitution of starch stearate increased with increasing water activity and the amount of hydrated salts. When water activity reached 0.61, the amount of addition was 80%, the degree of substitution reached maximum value of 0.111. The catalytic capability of enzyme is sensitive to pH values, and the highest degree of substitution could be obtained when the lyophozyme in a buffer solution with pH 7.1,when proper additive quantity of lyophozyme was 10.4%. The lipase that lyophilized with organic compounds such as Glycine and polyethylene glycol showed higher catalytic efficiency in ionic liquids than the enzyme without any protective agent. Lipase was found working most efficiently when polyethylene glycol was used as protective agent.

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[1] 李伟. 酶法合成单月桂酸丙二醇酯及其性质研究[D]. 广州: 华南理工大学, 2011: 20-28.
[2] 孙平, 陈健, 杨惠娟, 等. 酶法合成棕榈酸马铃薯淀粉酯[J]. 食品科技, 2012(4): 249-252.
[3] 王恺, 李书华. 柠檬酸酯淀粉最佳制备条件的研究[J]. 食品与机械, 2011, 27(4): 140-141.
[4] 钟涛, 乐长高, 谢宗波, 等. 碱性离子液体在有机合成中的应用研究进展[J]. 有机学, 2010, 30(7): 981-987.
[5] 卢璇璇. 离子液体介质中脂肪酶催化合成长链脂肪酸淀粉酯的研究[D]. 广州: 华南理工大学, 2013.
[6] LU Xuan-xuan, LUO Zhi-gang, YU Shu-juan, et al. Lipase-catalyzed synthesis of starch palmitate in mixed ionic liquids[J]. Journal of Agricultural and Food Chemistry, 2012, 60(36): 9 273-9 279.
[7] FERDJANI S, IONITA M, ROY B, et al. Correlation between thermostability and stability of glycosidases in ionic liquid[J]. Biotechnol Lett, 2011, 33(6): 1 215.
[8] ATSUSHI K, YUKI K, SHIORI I. Enzymatic synthesis of caffeic acid phenethyl ester analogues in ionic liquid[J]. Journal of Biotechnology, 2010, 148(2/3): 133-138.
[9] SUNG H H, MAI N L, YOON M K. Continuous production and in situ separation of fatty acid ester in ionic liquids[J]. Enzyme and Microbial Technology, 2010, 47(1/2): 6-10.
[10] 陆杨, 李在均, 蔡燕. 新型温控离子液体绿色介质生物催化合成乙酸辛酯香料[J]. 化学剂, 2010, 32(9): 793-798.
[11] 王永泽. 离子液体中脂肪酶催化酯交换合成和拆分的研究[D]. 杭州: 浙江大学, 2006.
[12] 王成涛, 丁晓英, 张佳婵, 等. 非水相介质杂醇油酶法转化天然酯类香料的研究[J]. 食品与机械, 2011, 27(6): 19-22.
[13] SUNIL S, ERIK H. Control of water activity in lipase catalysed esterification of chiral alkanoic acids[J]. Journal of Molecular Catalysis B: Enzymatic, 2009, 58(1): 6-9.
[14] MAREK A, UWE T B. Improving ascorbyl oleate synthesis catalyzed by Candida antarctica lipase B in ionic liquids and water activity control by salt hydrates[J]. Process Biochemistry, 2009, 44(3): 257-261.
[15] ROSA C D,MORANDIM M B,NINOW J L. Lipase-catalyzed production of fatty acid ethyl esters from soybean oil in compressed propane[J]. Bioresource Technology, 2009, 100(23): 5 818-5 826.
[16] 钟遇安, 姜土, 吴炜亮, 等. 酶法制备丙二醇单酯的研究[J]. 食品与机械, 2012, 28(5): 42-45.
[17] KLIBANOV A M. Improving enzymes by using them in organic solvents[J]. Nature, 2001, 409(6 817): 241-246.
[18] MARUYAMA T, NAGASAWA S, GOTO M. Poly (ethylene glyeol)-lipase complex that is catalytically active for alcoholysis reactions in ionic liquids[J]. Biotechnology Letters, 2002, 24(16): 1 341-1 345.
[19] SECUNDO F, CARREA G. Lipase activity and conformation in neat organic solvents[J]. Journal of Moleucular Catalysis: Enzymatic, 2002, 19(20): 93-102.



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