•  
  •  
 

Abstract

Established an UPLC method in determination the amino acids of dairy products, and detected the amino acid content of dairy products processed in different temperature, to identify the spray dried milk through the changes of amino acids contents and composition. The results showed that the calibration curve was linear in the range of 0.003 7~0.052 2 mg/mL of amino acids, the average recovery range of amino acid was from 97.45%~105.65%. It has the advantage of simple, rapid and accuracy in the determination amino acids. With the spray temperature changing, the content of amino acids were different. And the change of Glu, Leu, Cys were the most obvious. It can serve as the distinguish method of reconstituted milk.

Publication Date

7-28-2016

First Page

56

Last Page

60

DOI

10.13652/j.issn.1003-5788.2016.07.013

References

[1] HAENLEIN G F W. Nutritional value of dairy products of ewe and goat milk[J]. International Dairy Federation Special Issue, 2001: 159-178.
[2] LINDMARK-MNSSON H, SVENSSON U, PAULSSON M, et al. Influence of milk components, somatic cells and supplemental zinc on milk processability[J]. International Dairy Journal, 2000, 10(7): 411-424.
[3] ATHANASIA M Goula, KONSTANTINOS G Adamopoulos. Spray drying performance of a laboratory spray dryer for tomato powder preparation[J]. Drying Technology, 2003, 7(7):1 273-1 289.
[4] 宋贤聚. 低吸湿性杨梅粉喷雾干燥工艺的优化[J]. 食品与机械, 2013, 29(3): 226-229.
[5] 张景亮, 胡少新, 孙莹, 等. 复原乳检测方法的研究进展[J]. 食品科技, 2008, 12(4): 77-79.
[6] 迟骁玮, 陈志伟. 乳制品中复原乳检测方法的研究进展[J]. 中国酿造, 2011, 16(9): 4-7.
[7] 付洪涛, 欧阳华学, 雷绍荣. 高效液相色谱法测定复原乳中糠氨酸的含量[J]. 中国测试技术, 2008, 8(2): 103-104.
[8] SCHWARZ E L, ROBERTS W L, PASQUALI M. Analysis of plasma amino acids by HPLC with photodiode array and fluorescence detection[J]. Clinica Chimica Acta, 2005, 354(1/2):83-90.
[9] 杨俊, 王文辉, 李翼, 等. 柱前衍生超高效液相色谱法测定核桃仁中的氨基酸含量[J]. 食品与发酵工业, 2011, 37(2): 182-185.
[10] Waters公司北京实验室. Waters ACQUITY UPLCTM介绍[J]. 环境化学, 2004, 14(5): 605-611.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.