Abstract
To investigate ultrasonic method of total saponins from S.tetradonta and study the antioxidant activities. By using the single factor experiment and response surface methodology, taking he total saponin yields as the response value, the extraction procees was optimized. Meanwhile, UV spectrophotometry were used to determine the abilities of total saponins from S.tetradonta on scavenging DPPH·, ABTS· and FRAP assay. The results indicated that the optimal extraction conditions were as follows: ultrasonic time 40 min, ratio of water to material 30 mL/g, and ethanol concentration 77%. Under these conditions, the content of total saponins reached 3.1 mg/g. The total saponins from S.tetradonta had a strong ability to scavenge of DPPH·. And its total reducing power assays and ABTS· scavenging effect were relatively weak.
Publication Date
7-28-2016
First Page
141
Last Page
145
DOI
10.13652/j.issn.1003-5788.2016.07.033
Recommended Citation
Kai, OUYANG; Xianyuan, HE; Fei, CHEN; Yingru, HUANG; Maoqin, ZHOU; and Peng, CHENG
(2016)
"Study on optimization of extraction process for total saponins from S.tetradonta and its antioxidant activity,"
Food and Machinery: Vol. 32:
Iss.
7, Article 33.
DOI: 10.13652/j.issn.1003-5788.2016.07.033
Available at:
https://www.ifoodmm.cn/journal/vol32/iss7/33
References
[1] 张萃蓉, 潘世民, 韩建华, 等. 四棱草和四齿四棱草生长发育与生态环境的研究[J]. 中国中药杂志, 1996, 21(8): 467-468.
[2] 四川省中医药研究院南川药物种植研究所, 四川省中药材公司. 四川中药材栽培技术[M]. 重庆: 重庆出版社, 1988: 740-743.
[3] 窦辉, 彭树林, 李帮经, 等. 四齿四棱草根部的两个新二萜化合物[J]. 有机化学, 2004, 24(11): 1 469-1 471.
[4] 窦辉, 廖循, 陈昌祥, 等. 四齿四棱草根部环肽化合物的分离及鉴定[J]. 高等学校化学学报, 2004, 25(10): 1 849-1 852.
[5] 李帮经, 陈昌祥, 窦辉, 等. 四齿四棱草水提取物的化学成分研究[J]. 天然产物研究与开发, 2006, 18(1): 61-64, 87.
[6] 许柏艳, 李佥, 田晶, 等. 文冠果果壳皂甙的分离纯化及其抗氧化活性研究[J]. 食品工业科技, 2015, 36(15): 252-255, 269.
[7] 马春宇. 苦瓜总皂苷对2型糖尿病大鼠降糖、调脂作用的机制研究[D]. 沈阳: 辽宁中医药大学, 2014: 49-50.
[8] YU G J, CHOI I W, KIM G Y, et al. Anti-inflammatory potential of saponins derived from cultured wild ginseng roots in lipopolysaccharide-stimulated RAW 264.7 macrophages[J]. Int. Mol. Med., 2015, 35(6): 1 690-1 698.
[9] 程晓华, 熊玉卿. 五环三萜皂苷的药理作用研究进展[J]. 中草药, 2007, 38(5): 792-795.
[10] 令狐晨, 阿依吐伦·斯马义. 皂苷提取纯化及其抗氧化活性的研究进展[J]. 广州化工, 2015, 43(6): 7-9, 22.
[11] 董秀芳, 李楠, 韩冬, 等. 裙带菜孢子叶多糖的超声辅助提取工艺优化及其抗氧化活性研究[J]. 食品与机械, 2015, 31(4): 162-166, 177.
[12] 若源, 谢晶, 王婷, 等. 超声波辅助提取银杏叶中总黄酮的工艺优化[J]. 食品与机械, 2015, 31(1): 167-170.
[13] 董周永, 任辉, 周亚军. 松籽壳总黄酮超声提取工艺响应面优化[J]. 食品与机械, 2011, 27(5): 100-102.
[14] 王雨, 单杨, 李高阳. 超声波辅助提取野桂花蜜中多酚的工艺优化[J]. 食品与机械, 2015, 31(3): 189-193.
[15] 解红霞, 赵科, 张娜, 等. 响应面法优化芹叶铁线莲总皂苷超声提取工艺[J]. 中药材, 2014, 37(7): 1 269-1 271.
[16] 吴晖, 赖富饶, 胡筱波. 响应面分析法优化油菜花粉多糖提取工艺的研究[J]. 食品与机械, 2007, 23(5): 66-69,73.
[17] 邱丹缨, 温扬敏, 苏齐, 等. 兖州卷柏生物碱的抗氧化活性研究[J]. 天然产物研究与开发, 2015, 27(3): 442-445.
[18] RE R, PELLEGRINI N, PROTEGGENTE A, et al. Antioxidant activity applying an improved ABTS redical cation decolorization assay[J]. Free Radi. Biol. Med., 1999, 26(9/10): 1 231-1 237.
[19] 王蓓蓓, 段玉峰, 邵红军, 等. 红托竹荪多糖抗氧化活性的研究[J]. 天然产物研究与开发, 2012, 24(8): 1 122-1 125.
[20] 刘小攀, 田春莲, 覃基信, 等. Box-Behnken响应面法优化超声辅助提取忽地笑多糖工艺研究[J]. 中药材, 2015, 38(7): 1 520-1 523.
[21] 王玲丽, 滕红梅, 郭艳茹, 等. 响应面分析法优化超声提取细叶远志皂苷工艺研究[J]. 中药材, 2014, 37(4): 679-683.