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Abstract

In order to optimize the conditions of microwave assisted extraction of polysaccharides from Enteromorpha intestinals, the effect of 5 factors including microwave power, including the extraction duration, temperature, times of extraction and solid/liquid ratio on the extraction yield, which was investigated by the single factors in experiment. The extraction conditions were further optimized by the response surface methodology with the Design-Expert 8.0.5 software. The results showed that their effects were in the order of temperature > microwave power > times of extraction > extraction duration. The optimal extraction conditions of microwave assisted extraction of polysaccharides from E. intestinals were determined as followed: microwave power 500 W, extraction duration 15 min, temperature 90 ℃, extracting 2 times, solid/liquid ratio of 1∶30 (g/mL). Under these optimal conditions, the experimental yield was 11.38% with polysaccharides content of 31.34%. It provides a reference in the research of extraction technology of E. intestinals polysaccharides.

Publication Date

7-28-2016

First Page

152

Last Page

155

DOI

10.13652/j.issn.1003-5788.2016.07.035

References

[1] 王建伟, 阎斌伦, 林阿朋, 等. 浒苔(Enteramorpha prolifera)生长及抱子释放的生态因子研究[J]. 海洋通报, 2007, 26(2): 60.
[2] GAO Yuan, YUE Qin-yan, GAO Bao-yu, et al. Optimization preparation of activated carbon from Enteromorpha prolifra using response surface methodology and its adsorption studies of fluoroquinolone antibiotics[J]. Desalination and Water Treatment, 2015, 55(3): 624-636.
[3] LAHAYEL Marc, ROBICL Audrey. Structure and functional properties of Ulvan, a polysaccharide from Green Seaweeds[J]. Biomacromolecules, 2007, 8(6): 1 765-1 774.
[4] 焦丽丽. 肠浒苔多糖结构及生物活性研究[D]. 吉林: 东北师范大学, 2009: 59-64.
[5] 徐银峰, 王斌, 苏传玲, 等. 浒苔多糖的微波辅助提取工艺及抗氧化活性研究[J]. 浙江海洋学院学报: 自然科学版, 2011, 30(3): 211-216.
[6] 孙士红. 碱提浒苔多糖降血脂作用研究[J]. 中国现代药物应用, 2010, 4(15): 118-182.
[7] 王章姐, 郁万杨, 张梦茹. 植物多糖研究进展[J]. 亚太传统医药, 2014, 10(6): 39-40.
[8] 岳春, 李靖靖, 方永远. 虫草多糖微波辅助提取工艺的优化[J]. 食品与机械, 2014, 30(1): 192-195.
[9] 杨志伟, 王钰, 揭雅娇, 等. 微波辅助提取杜仲翅果籽粕蛋白的工艺优化[J]. 食品与机械, 2014, 30(1): 205-208.
[10] 王娟, 央金卓嘎, 黄惠华. 茶树花精油超高压、超声波与微波辅助萃取工艺优化[J]. 食品与机械, 2014, 30(2): 146-148, 180.
[11] 周海旭, 李忠海, 钟海雁, 等. 微波辅助乙醇提取樟树叶多酚工艺优化[J]. 食品与机械, 2013, 29(5): 161-163, 203.
[12] 陈铁壁, 肖乐, 杨盟盟, 等. 金桂花中总黄酮的微波辅助提取工艺优化[J]. 食品与机械, 2015, 31(3): 185-188.
[13] LI Sheng-nan, HAN Dan-dan, ROW Kyung Ho. Optimization of enzymatic extraction of polysaccharides from some marine algae by response surface methodology[J]. Korean Journal of Chemical Engineering, 2012, 29(5): 650-656.
[14] 陈小梅, 甘纯玑, 陈彩玲, 等. 响应面法优化微波辅助提取浒苔多糖工艺[J]. 食品研究与开发, 2011, 32(4): 44-48.
[15] 郭雷, 陈宇. 响应面法优化超声辅助提取浒苔多糖的工艺[J]. 食品科学, 2010, 31(16): 117-121.

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