•  
  •  
 

Abstract

Response surface analysis was proposed to optimize the ultrasound-assisted extraction process of crude polysaccharides from bulbils. The effects of solid-liquid ratio, ultrasonic power and extraction time on crude polysaccharide yield were investigated by single factor experiments. Response surface analysis was carried out by using the crude polysaccharide yield as response value and the box Behnken method to design the experiments. The optimum extraction conditions were obtained as follows: solid-liquid ratio, 18∶1 (mL/g), ultrasonic power, 760 W; extraction time, 23 min and the crude polysaccharides yield could be up to 60.68%, which agreed with the predicted value (the relative error is 2.03%). The results demonstrated that it is reasonable and practicable to optimize the ultrasonic extraction process using response surface methodology.

Publication Date

9-28-2016

First Page

149

Last Page

153

DOI

10.13652/j.issn.1003-5788.2016.09.037

References

[1] 都恒青. 常用中药材品种整理和质量研究(北方篇): 第二册[M]. 北京: 北京医科大学中国协和医科大学联合出版社, 1995: 1 089.
[2] 盛玮, 薛建平, 谢笔钧. 怀山药零余子化学成分及其营养评价[J]. 食品科技, 2009, 34(8): 76-79.
[3] 薛建平, 祝红蕾, 尧俊英. 铁棍山药及其零余子营养成分的比较研究[J]. 食品工业科技, 2008(8): 268-269.
[4] LIANG Xiao, HUANG Yue-qin, CHEN Jian-ping, et al. Antioxidant of polysaccharides in yam bulbils and their hypoglycemic effect in diabetic mice[J]. Agricultural Science & Technology, 2015, 16(7): 1 332-1 335.
[5] 李孟婕, 范秀萍, 吴红棉, 等. 翡翠贻贝粗多糖降血脂作用的研究[J]. 食品科学, 2012, 33(1): 257-261.
[6] 王娜, 冯艳风, 范会平, 等. 大枣粗多糖体外抗凝血活性的差异化研究[J]. 中国食品学报, 2013, 13(12): 34-39.
[7] 邱松山, 周天, 姜翠翠. 无花果粗多糖提取工艺及抗氧化活性研究[J]. 食品与机械, 2011, 27(1): 40-42.
[8] 傅冬和, 张影, 曾婷玉, 等. 洋葱水溶性多糖的提取工艺研究[J]. 食品与机械, 2013, 29(1): 125-127.
[9] 李宏睿, 陶浚, 董兵兵, 等. 超声波辅助提取香菇多糖工艺优化[J]. 食品与机械, 2010, 26(6): 138-140.
[10] 侯秀娟, 沈勇根, 徐明生, 等. 响应曲面法优化微波萃取化橘红多糖[J]. 中国食品学报, 2013, 13(3): 101-109.
[11] 付宏媛, 张坤生, 任云霞. 酶解法提取甘薯多糖技术研究[J]. 中国食品学报, 2008, 8(2): 128-132.
[12] 盛桂华, 周泉城. 超临界CO2萃取瓜蒌多糖工艺研究[J]. 食品工业科技, 2008, 29(5): 208-210.
[13] 李倩, 田春芳, 刘雯霞, 等. 超声波复合酶法提取软紫草多糖的工艺优化[J]. 中国现代应用药学, 2012, 29(6): 498-503.
[14] YUAN Qing-xia, XIE Yu-feng, WANG Wei, et al. Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves[J]. Carbohydrate Polymers, 2015, 128(5): 52-62.
[15] 秦培鹏, 刘涛. 猴头菌多糖热水浸提工艺研究[J]. 安徽农业科学, 2014, 42(15): 4 784-4 786.
[16] 金迪, 梁英, 孙工兵, 等. 植物多糖提取技术的研究进展[J]. 黑龙江八一农垦大学学报, 2011, 23(5): 76-79.
[17] 覃海元, 潘嫣丽, 覃梅珍. 热水与超声波提取鹿角灵芝多糖工艺比较研究[J]. 中国食品添加剂, 2011(3): 91-95.
[18] 崔玮, 张勇, 高海宁, 等. 响应面法优化果胶酶辅助提取锁阳原花青素的工艺[J]. 食品科学, 2016, 37(14): 1-9.
[19] 余洋定, 启航, 李冬梅, 等. 果胶酶辅助提取裙带菜孢子叶多糖的工艺条件优化[J]. 食品与机械, 2012, 28(1): 175-177.
[20] CHEN Hua-guo, ZHOU Xin, ZHANG Jun-zeng. Optimization of enzyme assisted extraction of polysaccharides from Astragalus membranaceus[J]. Carbohydrate Polymers, 2014, 111(13): 567-575.
[21] 贾丽艳, 任盛财. 复合酶法提取零余子多糖工艺的优化[J]. 山西农业大学学报: 自然科学版, 2013, 33(2): 130-135.
[22] 官波, 郑文诚. 山药多糖提取工艺的优化[J]. 食品与机械, 2010, 26(1): 98-101.
[23] BANIK R M, PANDEY K S. Selection of metal salts for alkaline phosphatase production using response surface methodology[J]. Food Research International, 2009, 42(4): 470-475.
[24] FAN Jie-ping, CAO Jing, ZHANG Xue-hong et al. Optimization of ionic liquid based ultrasonic assisted extraction of puerarin from Radix Puerariae Lobatae by response surface methodology[J]. Food Chemistry, 2012, 135(4): 2 299-2 306.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.