Establishment of a quantitative real-time PCR for detecting vvhA gene in Vibrio vulnificus

Authors

LI Dandan, Animal Quarantine Lab, Inspection & Quarantine Technology Center of Hainan Entry-Exit Inspection & Quarantine Bureau, Haikou, Hainan 570311, China
XU Yigang, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjinag 150001, China
QIU Suoping, Conghua Entry-Exit Inspection & Quarantine Bureau, Conghua, Guangdong 510900, China
WANG Yu, Animal Quarantine Lab, Inspection & Quarantine Technology Center of Chongqing Entry-Exit Inspection & Quarantine Bureau, Chongqing 404100, China
GAO Huijiang, Laboratory of Bovine Genetics and Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
GAO Shenyang, Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, Jinzhou, Liaoning 121001, China

Abstract

To establish a rapid assay for Vibrio vulnificus (V. vulnificus) detection, a quantitative real-time PCR (qRT-PCR) method was developed targeting vvhA gene of V. vulnificus . The results showed that the tests for 15 strains of different bacteria by using this method, only the ones of V. vulnificus were positive, indicating high specificity of qRT-PCR. Moreover, the sensitivity of this specific method was 15 CFU/mL. Further stability and reproducibility test results showed that the coefficient of variation of Ct values of four duplicates were less than 2%. In addition, a total 2 positive samples for V. vulnificus were detected from 156 clinical samples by this method, and the results were in accordance with those by SN/T 1870-2007 standard detections. Therefore, the qRT-PCR method provides a novel rapid and sensitive detection method for V. vulnificus infection.

Publication Date

9-28-2016

First Page

31

Last Page

33,70

DOI

10.13652/j.issn.1003-5788.2016.09.007

Recommended Citation

Dandan, LI; Yigang, XU; Suoping, QIU; Yu, WANG; Huijiang, GAO; and Shenyang, GAO (2016) "Establishment of a quantitative real-time PCR for detecting vvhA gene in Vibrio vulnificus," Food and Machinery: Vol. 32: Iss. 9, Article 7.
DOI: 10.13652/j.issn.1003-5788.2016.09.007
Available at: https://www.ifoodmm.cn/journal/vol32/iss9/7

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