Abstract
The bacteriostatic activity and bacteriostatic mechanism of thymol to Listeria monocytogenes were studied and the thymol-chitosan composite membrane was prepared to study the properties of the composite membrane and its preservation effect on pork. The results showed that the minimum inhibitory concentration of thymol on Listeria monocytogenes was 12.5 μg/mL. The experimental study of conductivity, nucleic acid and protein leakage found that thymol caused by mononuclear proliferative Leptospira cell membrane damage, so that bacterial cell inclusions caused by bacterial death. With the increase of thymol concentration (0.25%, 0.50%, 1.00%) in the thymol-chitosan composite membrane, the hardness and puncture strength of the membrane gradually decreased. The temperature and thymol concentration affected the removal of DPPH free radicals by composite membranes. At 25 ℃, the scavenging rate of 1.00% thymol-composite membrane was the highest at 25 ℃; the growth and reproduction of microbes in pork were reduced by the thymol-chitosan composite membrane, the total number of colonies decreased and the chilling period of pork was prolonged.
Publication Date
11-28-2017
First Page
104
Last Page
108
DOI
10.13652/j.issn.1003-5788.2017.11.022
Recommended Citation
Di, WANG; Renfeng, ZHANG; Bin, ZHANG; Bai, CHENG; Hui, XU; Chunlin, KE; and Maosheng, REN
(2017)
"The thymol-chitosan composite membrane and its application in pork preservation,"
Food and Machinery: Vol. 33:
Iss.
11, Article 22.
DOI: 10.13652/j.issn.1003-5788.2017.11.022
Available at:
https://www.ifoodmm.cn/journal/vol33/iss11/22
References
[1] 斐海闰, 韩笑, 曹学力. 百里香精油成分分析及其抗氧化和抑菌活性评价[J]. 中国食品学报, 2011, 11(5): 182-187.
[2] 王建清, 刘光发, 金政伟, 等. 百里香精油的抑菌作用及其对鲜切冬瓜的保鲜效果[J]. 包装技术与工程, 2009, 30(10): 1-4.
[3] SCOLLARD J, MCMANAMON O, SCHMALENBERGER A. Inhibition of Listeria Monocytogenes growth on freshcut pr-oduct with thyme essential oil and essentia oil compound verbenone[J]. Postharves Biology and Techno-logy, 2016, 120: 61-68.
[4] 樊明涛, 李亚琴. 百里香提取物抑菌作用影响因素的研究[J]. 江苏理工大学学报, 2001, 22(3): 13-16.
[5] 樊明涛, 陈锦屏. 百里香提取物抑菌特性的研究[J]. 微生物学报, 2001, 41(4): 501-504.
[6] 殷清华, 庄英帜, 严奉祥. 百里香酚的抗肿瘤作用[J]. 现代微生物学进展, 2010, 10(11): 2 073-2 075.
[7] ANIL Kumar Chauhan, SUN Chul Kang. Thymol disrupts the membrane integrity of Salmonella ser.typhim-urium in vitro and recovers infected macrophages from oxidative stress in an exvivo model[J]. Research in Microbiology, 2014, 165: 559-565.
[8] 连凯, 谈卫君, 赵月. 2002~2012年人和动物李斯特菌感染报告数据流行病学分析[J]. 中国人兽共患学报, 2014, 30(10): 1 033-1 038.
[9] 孙亚薇, 徐鹏, 平政, 等. 单核细胞增生李斯特菌感染所致炎症反应性疾病的研究进展[J]. 生物技术通讯, 2016, 27(6): 884-887.
[10] 周辉, 叶科元, 胡逸敏. 屎肠球菌HY07发酵液对冷却猪肉中单增李斯特菌的抑制作用研究[J]. 食品科技, 2015, 40(2): 145-148.
[11] 周伟, 刘国荣, 李平兰, 等. 植物乳杆L-1对单核细胞增生李斯特氏菌作用机理的研究[J]. 微生物学报, 2007, 47(2): 260-264.
[12] MOHAMMADREZA Rezaei Golestania, MEHRNAZ Rad, MOHAMMADREZA Bassami, et al. Analysis and evaluation of antibacterial effects of new herbal formulas,AP-001 and AP-002, against Escherichia coli O157:H7[J]. Life Sciences, 2015, 135: 22-26.
[13] 刘伟. 姜酚抑菌作用及姜酚-壳聚糖复合膜性质与应用研究[D]. 北京: 中国农业大学, 2015.
[14] ANJA Klancnik, SASA Piskernik, BARBARA Jersek, et al. Evaluation of diffusion and dilution methods to determine theantibacterial activity of plant extracts[J]. Journal of Microbiological Methods, 2010, 81(2): 121-126.
[15] 赵淑艳, 呼世斌, 吴焕利, 等. 山茱萸提取物抑菌活性成分稳定性的研究[J]. 食品科学, 2008, 29(1): 98-101.
[16] KONG Ming, CHEN Xi-guang, LIU Cheng-sheng, et al. Antibacterial mechanism of chitosan microspheres in a solid dispersing system against E. coli. Colloids and surfaces[J]. Biointerfaces, 2008, 65(2): 197-202.
[17] 曾媛媛, 周研, 谢晶, 等. 羧甲基壳聚糖/海藻酸钠共混膜对哈密瓜保鲜效果的影响[J]. 食品与机械, 2016, 32(4): 151-154.
[18] 张思聪, 夏文水, 王斌, 等. 不同分子量壳聚糖对高脂膳食小鼠血糖的调节作用[J]. 食品与机械, 2017, 33(4): 122-126.
[19] PENG Yong, WU Yan. Development of tea extracts and chitosan composite films for active packa Ging materials[J]. International Journal of Biological Macromolecules, 2013(59): 282-289.
[20] 卫生部编制. GB/T 4789.2—2016 食品安全国家标准 食品微生物学检验菌落总数的测定[S]. 北京: 中国标准出版社, 2016.
[21] 张晓鸣. 食品感官评定[M]. 北京: 中国轻工业出版社, 2013: 6-8.