Graded ethanol method extraction and the antioxidant activity comparison of flavonoids from crude propolis

Authors

JIANG Hongyu, Hunan Provincial Engineering Research Center for Ginkgo Biloba, Hunan University of Science and Engineering, Yongzhou, Hunan 425100, China
PAN Xue, Hunan Provincial Engineering Research Center for Ginkgo Biloba, Hunan University of Science and Engineering, Yongzhou, Hunan 425100, China
LI Muwu, Hunan Provincial Engineering Research Center for Ginkgo Biloba, Hunan University of Science and Engineering, Yongzhou, Hunan 425100, China
WANG Zongcheng, Hunan Provincial Engineering Research Center for Ginkgo Biloba, Hunan University of Science and Engineering, Yongzhou, Hunan 425100, China; College of Chemistry & Chemical Engineering, Central South University, Changsha, Hunan 410083, China

Abstract

The active ingredients of crude propolis from Yongzhou were extracted by graded ethanol method and investigated the scope of their activity. With flavonoids content as index, content and the antioxidant activity of them in the crude propolis from different extraction conditions was evaluated. The results showed that the quality and content of flavonoids in the crude propolis extracted with 40%～60% ethanol were higher. The content of flavonoids in the extracts by graded ethanol method reached 41.2% and the extraction yield of flavonoids was 25.81%. The antioxidant activity of flavonoids of crude propolis extracted with 50% and 60% ethanol were better than Vc, but the one in the crude propolis extracted with 30% ethanol was the worst. It illustrated the crude propolis contained different types of flavonoids. To obtain high active flavonoids of crude propolis, water should be chosen as extraction solvent to water-soluble flavonoids and 40%～70% ethanol should be chosen as extraction solvent to medium polarity flavonoids.

Publication Date

11-28-2017

First Page

152

Last Page

156

DOI

10.13652/j.issn.1003-5788.2017.11.032

Recommended Citation

Hongyu, JIANG; Xue, PAN; Muwu, LI; and Zongcheng, WANG (2017) "Graded ethanol method extraction and the antioxidant activity comparison of flavonoids from crude propolis," Food and Machinery: Vol. 33: Iss. 11, Article 32.
DOI: 10.13652/j.issn.1003-5788.2017.11.032
Available at: https://www.ifoodmm.cn/journal/vol33/iss11/32

References

[1] 罗火林, 刘星星, 龚上佶, 等. 不同地区蜂胶和树胶的红外谱图分析[J]. 光谱学与光谱分析, 2015, 35(11): 3 058-3 062.
[2] 张翠平, 平舜, 黄帅, 等. 蜂胶的地理来源、植物来源及化学成分的研究[J]. 中国药学杂志, 2013, 48(22): 1 889-1 892.
[3] 田进军, 薛艳, 候振健. 伪三元相图法制备O/W型蜂胶微乳[J]. 食品与机械, 2011, 27(3): 127-129.
[4] 徐响, 张红城, 董捷. 蜂胶功效成分研究进展[J]. 食品工业科技, 2008, 29(9): 286-289.
[5] 房柱. 蜂胶——黄酮化合物的宝库[J]. 蜜蜂杂志, 1998(9): 11-12.
[6] 潘娅, 郑德俊, 周莉玲, 等. 蜂胶生理活性与药理作用研究浅释[J]. 中华中医药学刊, 2004, 22(2): 381-382.
[7] SAWICKA D, CAR H, BORAWSKA M H, et al. The anticancer activity of propolis[J]. Folia Histochemica Et Cytobiologica, 2012, 50(1): 25-37.
[8] MASSARO C F, KATOULI M, GRKOVIC T, et al. Anti-staphylococcal activity of C-methyl flavanones from propolis of Australian stingless bees (Tetragonula carbonaria) and fruit resins of Corymbia torelliana (Myrtaceae)[J]. Fitoterapia, 2014, 95(2): 247-257.
[9] AGERO M B, GONZALEZ M, LIMA B, et al. Argentinean propolis from Zuccagnia punctata cav. (Caesalpinieae) exudates: phytochemical characterization and antifungal activity[J]. Journal of Agricultural & Food Chemistry, 2010, 58(1): 194-201.
[10] 张翠平, 胡福良. 蜂胶中的黄酮类化合物[J]. 天然产物研究与开发, 2009, 21(6): 1 084-1 090.
[11] 付英娟. 蜂胶中有效成分的提取及应用研究[D]. 杨凌: 西北农林科技大学, 2007: 18-31.
[12] 马士巧, 马海乐, 潘忠礼, 等. 黑蜂蜂胶不同极性提取物的体外抗氧化活性[J]. 中国食品学报, 2016, 16(8): 53-58.
[13] 张云香, 杨志孝, 安蔚, 等. 不同溶剂对蜂胶黄酮提取率影响的研究[J]. 时珍国医国药, 2006, 17(1): 9-10.
[14] 王宗成, 蒋玉仁, 刘小文, 等. 响应面优化生姜茎叶总黄酮提取工艺及其抗氧化活性研究[J]. 天然产物研究与开发, 2015, 27(9): 1 582-1 588.
[15] 欧阳凯, 何先元, 陈飞, 等. 四齿四棱草总皂苷提取工艺优化及抗氧化性研究[J]. 食品与机械, 2016, 32(7): 141-145.
[16] 刘安, 罗松, 李祁广, 等. 不同工艺制备辣椒籽油的体外抗氧化活性比较[J]. 食品与机械, 2016, 32(6): 168-170.
[17] 陆俊, 罗丹, 张佳琦, 等. 三叶木通不同部位多酚、黄酮含量及抗氧化活性比较[J]. 食品与机械, 2016, 32(8): 132-135.
[18] 梁巧楣. 蜂胶总黄酮的制备及含量测定[J]. 中国现代医学杂志, 2007, 17(17): 2 123-2 126.
[19] 安砚波, 王浩. 响应面法优化蜂胶总黄酮的提取工艺[J]. 中国蜂业, 2016, 67(9): 46-49.
[20] 玄红专, 胡福良. 不同地区蜂胶抗氧化活性与化学组分的研究进展[J]. 蜜蜂杂志, 2009, 29(2): 7-10.