•  
  •  
 

Abstract

The distribution of microbes in the process of green bean sprouts, and the treatment and effect of the two different types disinfectants were studied. The results showed that the total amount of microorganisms in the mung beans sprouts was 5.8 × 107 CFU/g, and the microbial species were mainly Klebsiella, Acinetobacter, Enterobacter and Pseudomonas. The total amount of microorganisms in the mung bean sprouts was decreased to 1.0 × 105 CFU/g when the effective chlorine concentration was 1 000~3 000 mg/L NaClO, while the model control group decreased to 1.0 × 103 CFU/g. The effect of Ag + was worse than NaClO, but it also showed a similar law. Using the scanning electron microscopy(SEM)to observe the sprouts, the surface of microbes formed a visible biofilm, which significantly enhanced the tolerance of bacteria to disinfectants.

Publication Date

7-28-2017

First Page

51

Last Page

55

DOI

10.13652/j.issn.1003-5788.2017.07.011

References

[1] 李建英, 田中艳, 周长军, 等. 绿豆芽菜萌发条件及物质含量测定[J]. 黑龙江农业科学, 2010(7): 37-40.
[2] 雷昊, 谢晶. 新鲜蔬菜采后清洗、包装处理研究进展[J]. 食品与机械, 2016, 32(6): 215-219.
[3] 严灿, 刘升, 贾丽娥, 等. 蔬菜冷链物流技术研究进展[J]. 食品与机械, 2015, 31(4): 260-265.
[4] WEISS A, HERTEL C, GROTHE S, et al.Characterization of the cultivable microbiota of sprouts and their potential for application as protective cultures[J]. Systematic & Applied Microbiology, 2007, 30(6): 483-493.
[5] PIRARD D, GREVE H D, HAESEBROUCK F, et al. O157:H7 and O104:H4 Vero/Shiga toxin-producing Escherichia coli, outbreaks: respective role of cattle and humans[J]. Veterinary Research, 2012, 43(1): 1-12
[6] 成黎. 新鲜蔬菜中的微生物污染危害、检测和控制方法研究进展[J]. 食品科学, 2015, 36(23): 347-352.
[7] 孔凡春, 陆胜民, 王群. 臭氧在果蔬保鲜和农残降解上的应用[J]. 食品与机械, 2003(5): 24-26.
[8] JIN H H, LEE S Y. Combined effect of aqueous chlorine dioxide and modified atmosphere packaging on inhibiting Salmonella Typhimurium and Listeria monocytogenes in mungbean sprouts[J]. Journal of Food Science, 2007, 72(9): 441-445.
[9] BARI M L, UKUKU D O, KAWASAKI T, et al. Combined efficacy of nisin and pediocin with sodium lactate, citric acid, phytic acid, and potassium sorbate and EDTA in reducing the Listeria monocytogenes population of inoculated fresh-cut produce[J]. Journal of Food Protection, 2005, 68(7): 1 381-1 387.
[10] YE Jian-xiong, KOSTRZYNSKA M, DUNFIELD K, et al. Control of Salmonella on sprouting mung bean and alfalfa seeds by using a biocontrol preparation based on antagonistic bacteria and lytic bacteriophages[J]. Journal of Food Protection, 2010, 73(1): 9-17.
[11] BARI M L, NAKAUMA M, TODORIKI S, et al. Effectiveness of irradiation treatments in inactivating Listeria monocytogenes on fresh vegetables at refrigeration temperature[J]. Journal of Food Protection, 2005, 68(2): 318-323.
[12] 许思笑, 马文娟. 人工模拟肠炎沙门氏菌在绿豆芽中的内化定殖能力及其消毒处理研究[J]. 现代食品科技, 2016(12): 93-98.
[13] 赵清风, 陈介南, 张林, 等. AgNO3对大肠杆菌和金黄色葡萄球菌的抗菌作用及机制[J]. 生物加工过程, 2011, 9(3): 52-56.
[14] 墙蔷, 倪红卫, 幸伟, 等. 银的抗菌作用机理[J]. 武汉科技大学学报, 2007, 30(2): 121-124.
[15] 刘重喜. 大豆根部内生放线菌的筛选、鉴定及其活性代谢产物研究[D]. 哈尔滨: 东北农业大学, 2013: 17-19.
[16] 费鹏, 白洪健, 程述震, 等. PCR-DGGE法分析婴儿肠道菌群多样性[J]. 食品与机械, 2013, 29(2): 60-63.
[17] FETT W F, COOKE P H. Scanning electron microscopy of native biofilms on mung bean sprouts[J]. Canadian Journal of Microbiology, 2003, 49(1): 45-50.
[18] BENDOUAH Z, BARBEAU J, HAMAD W A, et al. Biofilm formation by Staphylococcus aureus, and Pseudomonas aeruginosa, is associated with an unfavorable evolution after surgery for chronic sinusitis and nasal polyposis[J]. Otolaryngology-Head and Neck Surgery, 2006, 134(6): 991-996.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.