•  
  •  
 

Abstract

Antibacterial activity of Nα-lauroyl-L-arginate ethylester(LAE) against five pathogenic microorganisms of fruits and vegetables were investigated in this study. Bacteriostatic test in vitro was taken to research the antibacterial activity of LAE. Penicillium digitatum, Penicillium italicum, Botrytis cinerea, Alternaria alternate and Pectobacterium carotovorum subsp. Carotovorum were treated as the model bacteria of pathogenic microorganisms of fruits and vegetables, and the minimum inhibitory concentration(MIC) of LAE against these model bacteria were determined. The inhibition of LAE to the hypha growth for moulds and the bactericidal effect of LAE for bacteria were also determined. Then the combined antibacterial effects of LAE and other preservative were explored. The results showed that the MIC of LAE against Botrytis cinerea, Alternaria alternate, Penicillium italicum, and Penicillium digitatum were 400, 200, 400, 400 μg/mL respectively. When the concentration of LAE reached the MIC of each strain, the high mycelial inhibition rate of the four molds could be maintained at 100%(16 h), 78.2%(48 h), 81.8%(48 h), and 79.1%(48 h) respectively. The MIC of LAE against Pectobacterium carotovorum subsp. Carotovorum was 25 μg/mL, and LAE with the concentration of 50 μg/mL could completely kill the tested bacteria within 90 min. Morever, the combination of LAE and sodium chloride could exert the best inhibitory effect on the model bacteria.

Publication Date

7-28-2018

First Page

127

Last Page

131,193

DOI

10.13652/j.issn.1003-5788.2018.07.027

References

[1] MURIEL-GALET V, LOPEZ-CARBALLO G, GAVARA R, et al. Antimicrobial food packaging film based on the release of LAE from EVOH[J]. International Journal of Food Microbiology, 2012, 157(2): 239-244.
[2] HIGUERAS L, LOPEZ-CARBALLO G, HERNANDEZ-MUNOZ P, et al. Development of a novel antimicrobial film based on chitosan with LAE (ethyl-N alpha-dodecanoyl-L-arginate) and its application to fresh chicken[J]. International Journal of Food Microbiology, 2013, 165(3): 339-345.
[3] MURIEL-GALET V, LOPEZ-CARBALLO G, GAVARA R, et al. Antimicrobial effectiveness of lauroyl arginate incorporated into ethylene vinyl alcohol copolymers to extend the shelf-life of chicken stock and surimi sticks[J]. Food and Bioprocess Technology, 2015, 8(1): 208-217.
[4] 陈倩茹. 植物精油对樱桃番茄主要病原菌抑制效果的研究[D]. 杭州: 浙江大学, 2014: 3.
[5] SOYLU E M, KOSE F. Antifungal activities of essential oils against citrus black rot disease agent alternaria alternata[J]. Journal of Essential Oil Bearing Plants, 2015, 18(4): 894-903.
[6] RAYMOND L V, ZHANG M, KARANGWA E, et al. Mixed noble gas effect on cut green peppers[J]. International Agrophysics, 2013, 27(1): 75-79.
[7] 包英才, 王丹, 赵晓燕, 等. 鲜切青椒优势腐败菌的分离纯化及鉴定[J]. 食品工业科技, 2013, 34(3): 332-334, 338.
[8] 崔崇士, 远屈. 一株新的胡萝卜软腐欧文氏菌的分离和鉴定[J]. 微生物学报, 2004(2): 136-140.
[9] 刁明明, 齐丹萍, 张充, 等. 半乳糖基甘油月桂酸单酯的抑菌活性和稳定性[J]. 食品科学, 2016, 37(15): 1-6.
[10] 刘国荣, 郜亚昆, 王欣, 等. 双歧杆菌细菌素Bifidocin A对金黄色葡萄球菌的抑菌作用及其机制[J]. 食品科学, 2017, 38(17): 1-7.
[11] RUIZ-PEREZ N J, GONZALEZ-AVILA M, SANCHEZ-NAVARRETE J, et al. Antimycotic Activity and genotoxic evaluation of citrus sinensis and citrus latifolia essential oils[J]. Scientific Reports, 2016, 6: 25 371.
[12] JOUSIMIES-SOMER H. Wadsworth-Ktl anaerobic bacteriolo-gy manual[M]. Singapore: Star Publishing Pte Ltd, 2002: 114-118.
[13] XU Wen-tao, QU Wei, HUANG Kun-lun, et al. Antibacterial effect of Grapefruit Seed Extract on food-borne pathogens and its application in the preservation of minimally processed vegetables[J]. Postharvest Biology and Technology, 2007, 45(1): 126-133.
[14] 张辉. 食品级月桂酸单甘油酯微乳体系的构建及其抑菌研究[D]. 杭州: 浙江大学, 2009: 38-39.
[15] 买尔哈巴·塔西帕拉提. 百里香酚微乳体系构建及其抑菌、抗氧化活性研究[D]. 杭州: 浙江大学, 2015: 36.
[16] 李贵玲, 刘根焰, 陈寅, 等. 大蒜素联合不同药物对多重耐药鲍曼不动杆菌的体外抗菌作用[J]. 中国临床药理学杂志, 2011, 27(10): 752-754.
[17] DE SOUSA J P, DE AZEREDO G A, TORRES R D, et al. Synergies of carvacrol and 1, 8-cineole to inhibit bacteria associated with minimally processed vegetables[J]. International Journal of Food Microbiology, 2012, 154(3): 145-151.
[18] ODDS F C. Synergy, antagonism, and what the chequerboard puts between them[J]. Journal of Antimicrobial Chemothera-py, 2003, 52(1): 1.
[19] SWAN G, DEAN G, HOCKRIDGE M, et al. Antimicrobial susceptibility in terms of the minimal inhibitory concentration (MIC) of LAE and Mirenat-N[J]. Journal of Applied Microbiology, 2004, 17(1): 28-39.
[20] BECERRIL R, MANSO S, NERIN C, et al. Antimicrobial activity of Lauroyl Arginate Ethyl (LAE), against selected food-borne bacteria[J]. Food Control, 2013, 32(2): 404-408.
[21] SONI K A, NANNAPANENI R, SCHILLINGM W, et al. Bactericidal activity of lauric arginate in milk and Queso Fresco cheese against Listeria monocytogenes cold growth[J]. Journal of Dairy Science, 2010, 93(10): 4 518-4 525.
[22] CORONEL-LEON J, LOPEZ A, ESPUNY M J, et al. Assessment of antimicrobial activity of N-alpha-lauroyl arginate ethylester (LAE (R)) against Yersinia enterocolitica and Lactobacillus plantarum by flow cytometry and transmission electron microscopy[J]. Food Control, 2016, 63: 1-10.
[23] 中华人民共和国国家卫生和计划生育委员会. GB 2760—2014 食品安全国家标准 食品添加剂使用标准[S]. 北京: 中国标准出版社, 2014: 201-268.
[24] SHAW D, SWAN G, HOCKRIDGE M, et al. Nα-lauroyl-L-arginine ethyl ester monohydrochloride In vitro stability[J]. Food and Chemical Toxicology, 2003, 30(2): 145-159.
[25] RUCKMAN S A, ROCABAYERA X, BORZELLECA J F, et al. Toxicological and metabolic investigations of the safety of N-alpha-lauroyl-L-arginine ethyl ester monohydrochloride (LAE)[J]. Food and Chemical Toxicology, 2004, 42(2): 245-259.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.