Abstract
A natural heparin-like compound was isolated from swim bladder and its anticoagulant activities were determined. The response surface analysis based on Box-Behnken design was adopted to examine effects of protease consumption, extracting time and temperature on heparin-like compound extraction. The anticoagulant activity of swim bladder heparin-like compound was evaluated by APTT, PT and TT method. Consequently, the optimum conditions were as follows. The concentration of protease from Bacillus licheniformis was 5.4 mg/mL, extracted at 50 ℃ for 20 h. Under the control of these conditions, the predicted extraction rate by mathematical model was (1.79±0.05)%, and the standard error was 3.24%. In addition, the heparin-like compound from swimming bladder (HSB) showed negligible in vitro anticoagulant activity, and played a role through the exogenous coagulation pathway and the common coagulation pathway.
Publication Date
7-28-2018
First Page
151
Last Page
157,219
DOI
10.13652/j.issn.1003-5788.2018.07.032
Recommended Citation
Siyi, ZHOU; Yi, QU; Saiyi, ZHONG; Zhenxing, DU; Weimin, SU; Suhua, CHEN; Pengzhi, HONG; and Xiaohe, WU
(2018)
"Optimization of Extraction process of heparinoid from swim bladder and evaluation its anticoagulant activity,"
Food and Machinery: Vol. 34:
Iss.
7, Article 32.
DOI: 10.13652/j.issn.1003-5788.2018.07.032
Available at:
https://www.ifoodmm.cn/journal/vol34/iss7/32
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