•  
  •  
 

Abstract

In order to study the volatile flavor of soymilk, a soymilk simulation system with soybean oil body as substrate was constructed, The effects of different pH on the components, particle size and flavor of soybean oil body extracted from soybean milk were studied in the oil body extraction process. The results showed that when the extraction pH increased from 7 to 11, the content of oil body protein decreased by 38.40%, the neutral fat content increased by 7.26%, and the phospholipid content decreased by 49.31%. The oil particle size decreased gradually by 89.2 nm and tended to be stable. Volatile flavor substances were also gradually reduced from 129.80 μg/L to 52.17 μg/L. When the pH > 9, the effect of lipoxygenase in extracted oil body was negligible. According to the comparison above, the purity and stability of oil body extracted under the condition of pH 10 were better, and the flavor substances contained in the oil body were also less. It can be seen that the oil body extracted at pH 10 is the most suitable substrate component of soybean milk simulation system.

Publication Date

9-28-2018

First Page

15

Last Page

18

DOI

10.13652/j.issn.1003-5788.2018.09.004

References

[1] IWANAGA D, GRAY D A, FISK I D, et al. Extraction and characterization of oil bodies from soy beans: A natural source of pre-emulsified soybean oil[J]. Journal of Agricuture and Food Chemistry, 2007(55): 8 711-8 716.
[2] ANDRE E, HOU K W. The presence of a lipid oxidase in soybean[J]. Compte Rendu Acad Sci(Paris), 1932(194): 645-647.
[3] ZHUANG Hong, HILDEBRAND D F, ANDERSEN R A, et al. Effects of polyunsaturated free fatty acids and esterified linoleoyl derivatives on oxygen consumption and c6 aldehyde formation with soybean seed homogenates[J]. Journal of Agricultural and Food Chemistry, 1991, 39(8): 1 357-1 364.
[4] NISHIBA Y, FURUTA S, HAJIKA M, et al. Hexanal accumulation and DETBA value in homogenate of soybean seeds lacking two or three lipoxygenase isozymes[J]. J. Agric. Food Chem., 1995(43): 738-741.
[5] SHI Xiao-di, LI Jing-yan, WANG Shu-ming, et al. Flavor characteristic analysis of soymilk prepared by different soybean cultivars and establishment ofevaluation method of soybean cultivars suitable for soymilk processing[J]. Food Chemistry, 2015, 185: 422-429.
[6] YUAN Shao-hong, CHANG Kow-china. Selected odor compounds in soymilk as affected by soybean materials and direct steam injection[J]. Food Sci., 2007, 72: 481-486.
[7] WILKENS W F, MATTICK L R, HAND D B. Effect of processing method on oxidative off-flavors of soybean milk[J]. Food Technol, 2011, 21: 1 630-1 634.
[8] ANTHONY H C Huang. Oil bodies and oleosins in seeds[J]. Annual Review of Plant Biology, 2003, 43(4): 177-200.
[9] 陈业明, 赵路苹, 熊小辉, 等. 热处理对大豆油体表面的油体蛋白和外源性蛋白影响[J]. 食品工业科技, 2014, 35(22): 106-113.
[10] 赵路苹, 陈业明, 张彩猛, 等. 提取条件对大豆油体表面蛋白质去除效果的研究[J]. 大豆科学, 2013, 32(6): 835-839.
[11] 陈雅静, 赵路苹, 华欲飞, 等. 植物种子油体的提取及其性质的初步研究[J]. 中国粮油学报, 2017, 32(5): 62-67.
[12] 曹艳芸, 陈业明, 华欲飞. 高碱性提取的大豆油体的成分变化研究[J]. 中国粮油学报, 2014, 29(3): 37-41.
[13] JUNG D M, EBELLER S E. Headspace Solid-Phase microextraction method for the study of the volatility of selected flavor compounds[J]. Journal of Agricultural and Food Chemistry, 2003(51): 200-205.
[14] LAEMMLI U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature, 1970, 227: 680-685.
[15] LIN Li-jen, TAI S S K, PENG Chi-chung, et al. Steroleosin, a sterol-binding dehydrogenase in seed oil bodies[J]. Plant Physiology, 2002, 128(4): 1 200-1 211.
[16] CHEN Ye-ming, ONO T. Simple extraction method of non-allergenic intact soybean oil bodies that are thermally stable in an aqueous medium[J]. Journal of Agricultural and Food Chemistry, 2010, 58: 7 402-7 407.
[17] TING J T, LEE K, RATNAYAKE C, et al. Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents[J]. Planta, 1996, 199(1): 158-165.
[18] KOBAYASHI A, TSUDA Y, HIRATA N, et al. Aroma const ituent s of soybean[ G ly cine max (L. ) Mer ril] milk lacking lipoxygenase isozymes[J]. J. Agric. Food Chem., 1995, 43: 2 449-2 452.
[19] YUAN Shao-hong, CHANG S K. Selected odor compounds in soymilk as affected by chemical composition and lipoxygenases in five soybean materials[J]. Agricultural and Food Chemistry, 2007, 55(2): 426-431.
[20] MEYNIER A, RAMPON V, DALGALARRONDO M, et al. Hexanal and t-2-hexenal form covalent bonds with whey proteins and sodium caseinate in aqueous solution[J]. International Dairy Journal, 2004, 14(8): 681-690.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.