Rice bran polysaccharides prevent DSS-induced colitis through MAPK signaling pathway in mice

Authors

LIU Jing, College of Chemical Biology and Environmental Engineering, Xiangnan University, Chenzhou, Hunan 423000, China; Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China
GUO Ting, Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China
GUO Tianyi, Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China; National Engineering Laboratory of Rice and By-Products Deep Processing, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China
ZOU Jiawen, Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China; National Engineering Laboratory of Rice and By-Products Deep Processing, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China
ZHOU Xiaotian, Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China; National Engineering Laboratory of Rice and By-Products Deep Processing, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China
HE Jie, Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China
SHI Yuhong, Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China
LUO Feijun, Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China; National Engineering Laboratory of Rice and By-Products Deep Processing, College of Food Science and Engineering, Central South Forestry University, Changsha, Hunan 410004, China

Abstract

To assess the anti-inflammatory function and possible molecular mechanism of rice bran polysaccharide (RBP), DSS-induced colitis model was used in ICR mice. Pathological changes and gene expression were analyzed by histopathological analysis, RT-qPCR and Western blotting among of different experiment groups. The results showed that 500 mg/kg/day RBP could significantly improve the health status of mice and reduced colonic inflammation. RBP reduced the disease active index (DAI) score of DSS-induced mice and alleviated the damage of pathological tissue, with the improvement of the inflammation-related biochemical indicators. qRT-PCR analysis showed that RBP could decrease the mRNA expression level of inflammatory factors, including TNFα, IL-1β, Cox-2 and iNOS in colon tissues. Western blotting analysis further confirmed the efficacy of RBP inhibiting the expression level of inflammatory factor protein. Moreover, RBP could also inhibit MAPK signaling pathway. The results suggested that RBP had anti-inflammatory effect, and its molecular mechanism might be related to down-regulating the expression of inflammatory factors via inhibiting MAPK signaling pathway.

Publication Date

1-28-2019

First Page

32

Last Page

40

DOI

10.13652/j.issn.1003-5788.2019.01.007

Recommended Citation

Jing, LIU; Ting, GUO; Tianyi, GUO; Jiawen, ZOU; Xiaotian, ZHOU; Jie, HE; Yuhong, SHI; and Feijun, LUO (2019) "Rice bran polysaccharides prevent DSS-induced colitis through MAPK signaling pathway in mice," Food and Machinery: Vol. 35: Iss. 1, Article 7.
DOI: 10.13652/j.issn.1003-5788.2019.01.007
Available at: https://www.ifoodmm.cn/journal/vol35/iss1/7

References

[1] 肖云, 张迎庆, 糜志远. 米糠多糖提取纯化工艺的研究进展[J]. 食品与药品, 2012, 14(6): 448-450.
[2] 聂莹, 罗非君, 曾晓楠. 米糠多糖生理功能研究及应用新进展[J]. 粮食与油脂, 2015(11): 10-13.
[3] 汪艳, 吴曙光, 周杰, 等. 和活性分析[J]. 生命科学研究, 2000, 4(3): 273-277.
[4] ITO E, TAKEO S, KADO H, et al. Studies on an antitumor polysaccharide RBS derived from rice bran. I. Preparation, physico-chemical properties, and biological activities of RBS [J]. Yakugaku Zasshi, 1985, 105(2): 188-193.
[5] 朱丽丹. 基于离子液体的米糠多糖硫酸酯化及其抗肿瘤、免疫活性研究[D]. 无锡: 江南大学, 2014: 1-4.
[6] 朱丽丹, 王莉, 徐逸木, 等. 米糠多糖及硫酸酯化米糠多糖的体外免疫应答和抗肿瘤活性研究[J]. 中国粮油学报, 2015, 30(3): 35-40.
[7] 汪艳, 吴曙光, 徐伟, 等. 米糠多糖抗肿瘤作用及其作用的部分机制[J]. 中国药理学通报, 1999, 15(1): 70-72.
[8] 姜元荣, 姚惠源, 王勇, 等. 米糠多糖RBP及RBP-Ⅱ对小鼠体外细胞因子分泌的影响研究[J]. 粮食科技与经济, 2014, 39(4): 61-63.
[9] 魏明, 王晨, 杨超英, 等. 酶法协同超声波提取米糠多糖及其抗氧化活性研究[J]. 中国油脂, 2015, 40(1): 78-82.
[10] 张秀媛, 何扩, 王丽霞, 等. 张杂谷米糠多糖微波提取及其对肥胖大鼠肝脏自由基代谢的影响[J]. 中国粮油学报, 2016, 31(5): 38-42.
[11] 丘成桢. 米糠多糖与黄芪多糖单糖组分鉴定及对机体免疫功能的影响[J]. 中国家禽, 2015, 37(10): 57-60.
[12] 许旭. 米糠多糖的提取、免疫调节活性及其口含片制备工艺研究[D]. 武汉: 武汉轻工大学, 2014: 42-43.
[13] KAHLON T, SAUNDERS R, CHOW F, et al.Influence of rice bran, oat bran and wheat bran on cholesterol and triglycerides in hamsters [J]. Cereal Chemistry, 1990, 67(5): 439-443.
[14] 胡忠泽, 金光明, 王立克, 等. 米糠多糖对糖尿病小鼠的降血糖作用研究[J]. 中国粮油学报, 2006, 21(4): 21-24.
[15] HU Tao, LIN Qin-lu, GUO Ting, et al. Polysaccharide isolated from Phellinus linteus mycelia exerts anti-inflammatory effects via MAPK and PPAR signaling pathways[J]. Carbohydr Polym, 2018, 200: 487-497.
[16] NIE Ying, LIN Qin-lu, LUO Fei-jun. Effects of non-starch polysaccharides on inflammatory bowel disease[J]. Int J Mol Sci, 2017, 18(7): 1 372-1 395.
[17] CASANOVA M J, CHAPARRO M, DOMENECH E, et al.Safety of thiopurines and anti-TNF-alpha drugs during pregnancy in patients with inflammatory bowel disease[J]. Am J Gastroenterol, 2013, 108(3): 433-440.
[18] GRIVENNIKOV S, GRETEN F, KARIN M. Immunity, inflammation, and cancer[J]. Cell, 2010, 140(6): 883-899.
[19] XU Bei-lei, ZHANG Gui-jun, JI Yu-bin. Active compon-ents alignment of Gegenqinlian decoction protects ulcerative colitis by attenuating inflammatory and oxidative stress[J]. J Ethnopharmacol, 2015, 162: 253-60.
[20] MIRANDA K M, ESPEY M G, WINK D A. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite[J]. Nitric Oxide, 2001, 5(1): 62-71.
[21] LIU Bo, LIN Qin-lu, YANG Tao, et al. Oat β-glucan ameliorates dextran sulfate sodium (DSS)-induced ulcerative colitis in mice[J]. Food Func, 2015, 6(11): 3 454-3 463.
[22] CHAPARRO M, GUERRA I, MUNOZ-LINARES P, et al. Systematic review: antibodies and anti-TNF-alpha levels in inflammatory bowel disease[J]. Aliment Pharmacol Ther, 2012, 35(9): 971-986.
[23] MCCARTHY J, O'NEILL MJ, BOURRE L, et al. Gene silencing of TNF-alpha in a murine model of acute colitis using a modified cyclodextrin delivery system[J]. J Control Release, 2013, 168(1): 28-34.
[24] MCALINDON M E, HAWKEY C J, MAHIDA Y R. Expression of interleukin 1 beta and interleukin 1 beta converting enzyme by intestinal macrophages in health and inflammatory bowel disease[J]. Gut, 1998, 42(2): 214-219.
[25] DINARELLO C A, WOLFF S M. The role of interleukin-1 in disease[J]. N Engl J Med, 1993, 328(2): 106-113.
[26] LUDWICZEK O, VANNIER E, BORGGRAEFE I, et al. Imbalance between interleukin-1 agonists and antagonists: relationship to severity of inflammatory bowel disease[J]. Clin Exp Immunol, 2004, 138(2): 323-329.
[27] ANDUJAR I, RIOS J L, GINER R M, et al. Beneficial effect of shikonin on experimental colitis induced by dextran sulfate sodium in BALB/c mice[J]. Evid Based Complement Alternat Med, 2012, 2 012: 271-606.
[28] THOMAS S, METZKE D, SCHMITZ J, et al. Anti-inflammatory effects of Saccharomyces boulardii mediated by myeloid dendritic cells from patients with Crohn's disease and ulcerative colitis[J]. Am J Physiol Gastrointest Liver Physiol, 2011, 301(6): G1 083-G1 092.
[29] SEO M J, LEE Y J, HWANG J H, et al. The inhibitory effects of quercetin on obesity and obesity-induced inflammation by regulation of MAPK signaling[J]. J Nutr Biochem, 2015, 26(11): 1 308-1 316.
[30] EMAMI H, VUCIC E, SUBRAMANIAN S, et al. The effect of BMS-582949, a P38 mitogen-activated protein kinase (P38 MAPK) inhibitor on arterial inflammation: a multicenter FDG-PET trial [J]. Atherosclerosis, 2015, 240(2): 490-496.
[31] SHI Li-ming, LIN Qin-lu, LI Xin-hua, et al. Alliin, a garlic organosulfur compound, ameliorates gut inflamm-ation through MAPK-NF-κB/AP-1/STAT-1 inactivation and PPAR-γ activation[J]. Mol Nutr Food Res, 2017, 61(9): 1-12.
[32] GUO Tian-yi, LIN Qin-lu, LI Xin-hua, et al. Octacosanol attenuates inflammation in both RAW264. 7 macrophages and a mouse model of colitis[J]. J Agric Food Chem, 2017, 65(18): 3 647-3 658.
[33] ROMAN J, RITZENTHALER J D, FENTON M J, et al. Transcriptional regulation of the human interleukin 1beta gene by fibronectin: role of protein kinase C and activator protein 1 (AP-1) [J]. Cytokine, 2000, 12(11): 1 581-1 596.
[34] RAHMAN I. Regulation of nuclear factor-kappa B, activator protein-1, and glutathione levels by tumor necrosis factor-alpha and dexamethasone in alveolar epithelial cells[J]. Biochem Pharmacol, 2000, 60(8): 1 041-1 049.
[35] BOND M, FABUNMI R P, BAKER A H, et al. Synergistic upregulation of metalloproteinase-9 by growth factors and inflammatory cytokines: an absolute requirement for transcription factor NF-kappa B[J]. FEBS Lett, 1998, 435(1): 29-34.