Quick determination of 3-methyl-quinoxaline-2-carboxylic acid residues in animal-derived food by alkali hydrolysis-solid phase extraction-liquid chromatography-tandem mass spectrometry

Authors

LIU Di, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
HAN Li, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
ZENG Ni, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
YU Tingting, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
WANG Heng, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
WANG Bin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
RONG Mao, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China

Abstract

A rapid quasi-deterministic quantitative method for the determination of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) residues in olaquindox metabolites of animal-derived foods was developed, by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The sample tissue was extracted by alkaline hydrolysis to extract MQCA, purified by anion solid phase extraction column, and detected by LC-MS/MS, and then quantified by internal standard method. The results showed that MQCA had a good linear relationship in the concentration range of 0.5～50.0 ng/mL, and the correlation coefficient was 0.999 7. The detection limit of MQCA was 0.1 μg/kg. The recovery rate was 95.6%～108.2% at the addition level of 0.1, 0.2 and 1.0 μg/kg, and the relative standard deviation was 3.4%～14.3% (n=6). In conclusion, the detection method above was accurate， rapid and sensitive, and suitable for detecting and confirming the residual amount of MQCA in animal foods.

Publication Date

11-28-2019

First Page

70

Last Page

74,93

DOI

10.13652/j.issn.1003-5788.2019.11.014

Recommended Citation

Di, LIU; Li, HAN; Ni, ZENG; Tingting, YU; Heng, WANG; Bin, WANG; and Mao, RONG (2019) "Quick determination of 3-methyl-quinoxaline-2-carboxylic acid residues in animal-derived food by alkali hydrolysis-solid phase extraction-liquid chromatography-tandem mass spectrometry," Food and Machinery: Vol. 35: Iss. 11, Article 12.
DOI: 10.13652/j.issn.1003-5788.2019.11.014
Available at: https://www.ifoodmm.cn/journal/vol35/iss11/12

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