Abstract
Ultrafiltration, macroporous resin, gel filtration chromatography and semi preparative liquid chromatography were used to separate and purify the enzymatic hydrolysate of Chinese roundabout meat. DPPH free radical scavenging rate, ABTS radical scavenging rate, iron reduction capacity (FRAP) and oxygen free radical absorbability (ORAC) were used as indicators to prepare antioxidant peptides with high purity and strong antioxidant activity. Enzyme activity and MDA production were measured. The results showed that the antioxidant activity of the enzyme hydrolysate after ultrafiltration was the strongest when the molecular weight was ＜1 kDa. Macroporous resin, gel filtration chromatography and semi preparative liquid chromatography were used to isolate the mixture containing antioxidant peptides (the purity of antioxidant peptide reached 90%). In vitro antioxidant activity showed that DPPH free radical scavenging rate, ABTS free radical scavenging rate and ORAC of the antioxidant peptide were the highest, and there was no significant difference between ORAC and glutathione (P＞0.05). Through the determination of antioxidant enzymes and MDA increase value in mice, except for GSH-Px, there was no significant difference between the test group and glutathione positive control group (P>0.05), indicating that the anti-inflammatory effect of this study was obtained. Oxidized peptide has the same antioxidative effect as glutathione, and has deep development and utilization value.
Publication Date
12-28-2019
First Page
151
Last Page
157
DOI
10.13652/j.issn.1003-5788.2019.12.028
Recommended Citation
Mingze, WU; Xiao, WANG; Xianghao, HU; Qinglin, MA; Yanan, LI; Danhua, DONG; Peng, GAO; and Long, DAI
(2019)
"Purification of antioxidant peptides from the meat of Oncomelania sinensis and its in vivo and in vitro of mice activity,"
Food and Machinery: Vol. 35:
Iss.
12, Article 28.
DOI: 10.13652/j.issn.1003-5788.2019.12.028
Available at:
https://www.ifoodmm.cn/journal/vol35/iss12/28
References
[1] 杨丽丽, 王斌, 朱化化, 等. 中华圆田螺抗菌肽理化性质及抑癌活性测定[J]. 食品研究与开发, 2013, 34(20): 5-8.
[2] 郭紫峰, 艾欢. 中华圆田螺及其生物活性物质[J]. 食品研究与开发, 2015, 36(12): 132-134.
[3] 郑汉丰, 李家乐. 浙江地区3种淡水经济贝类的营养成分分析与评价[J]. 中国农学通报, 2012, 28(2): 78-82.
[4] 王永闯, 肖兴军. 氧化应激在周围神经病变发病机制中的作用[J]. 卒中与神经疾病, 2016(1): 73-74.
[5] 蔡文灿, 王海, 李瑞青, 等. 类风湿性关节炎患者氧化还原物水平的测定[J]. 南方医科大学学报, 2005(6): 749-750.
[6] 邓嘉成. 磷酸果糖激酶缺陷损伤类风湿性关节炎T细胞ATP的产生、自噬与氧化还原平衡[J]. 生理科学进展, 2014(1): 31.
[7] 王少平, 宋晓光, 李秉润, 等. 雄土鳖虫活性物质分离工艺考察[J]. 中国医院药学杂志, 2017, 37(21): 2 135-2 138.
[8] SAMAD N B, DEBNATH T, YE M. HASNAT A, LIM B O. In vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts[J]. Asian Pacific Journal of Tropical Biomedicine, 2014, 4(10): 807-815.
[9] DAUTHAL P, MUKHOPADHYAY M. Antioxidant activity of phytosynthesized biomatrix-loaded noble metallic nanoparticles[J]. Chinese Journal of Chemical Engineering, 2018, 26(5): 1 200-1 208.
[10] 郭晓青, 吴金鸿, 刘畅, 等. 明日叶的茎与叶主要抗氧化成分含量及抗氧化性比较[J]. 食品与发酵工业, 2013, 39(8): 122-127.
[11] 戴卉卿, 陈承瑜, 杨滨. 22种黄酮、酚酸类化合物和9种中药清除AAPH活性考察[J]. 中国中药杂志, 2010, 35(17): 2 296-2 302.
[12] 张闽, 董文宾, 张小强, 等. 猪皮明胶肽体内抗氧化功效的研究[J]. 中国酿造, 2012, 31(11): 70-72.
[13] 徐鹏, 陈尚海, 张亮, 等. 黑胸散白蚁不同群体在形态分类特征上的差异性研究[J]. 应用昆虫学报, 2019(3): 563-578.
[14] 秦垂新, 姚松君, 唐青涛, 等. 抗氧化肽及其化学活性测定方法的研究概述[J]. 食品工业科技, 2014, 35(1): 394-400.
[15] 牛瑞, 于建生. 鳕鱼多肽的抗氧化活性及其分离纯化[J]. 食品与生物技术学报, 2010, 29(4): 562-566.
[16] 李阅兵, 孙立春, 刘承初, 等. 几种海水和淡水贝类的大宗营养成分比较研究[J]. 上海海洋大学学报, 2012, 21(2): 297-303.
[17] 曹壮, 李文钊, 王强, 等. 蛋清抗氧化肽分离纯化及结构鉴定[J]. 食品工业科技, 2019, 40(4): 82-85.
[18] 李晓英, 李勇, 周淑青, 等. 两种淡水螺肉的营养成分分析与评价[J]. 食品科学, 2010, 31(13): 276-279.