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Abstract

In order to select excellent strains for the fermentation of Chinese bayberry wine in Hunan province, 172 strains of yeast were isolated from fruits, leaves and soil of bayberry in different arbutus orchard. The three strains with excellent comprehensive performance were screened at four levels by chromogenic method, duchenne tube method, sugar fermentation method and bayberry wine fermentation method. It was identified as Saccharomyces cerevisiae RY1, Hanseniaspora opuntiae DY5 and Hanseniaspora pseudoquilliermondii JW14 by morphology and molecular biology. Through fermentation performance studies, it was found that all 3 yeasts were able to withstand an environment with a soluble solid content (SSC) of 32%, an SO2 addition of 200 mg/L, an alcohol content of 16% Vol, and a pH of 2.0. RY1 yeast has strong fermentation performance and tolerance. It is suitable for fermenting bayberry wine. DY5 and JW14 yeast have good aroma-producing ability, and it is suitable for auxiliary fermentation to enhance the aroma of bayberry wine.

Publication Date

3-28-2019

First Page

41

Last Page

47,62

DOI

10.13652/j.issn.1003-5788.2019.03.007

References

[1] 陈方永. 我国杨梅研究现状与发展趋势[J]. 中国南方果树, 2012, 41(5): 31-36.
[2] 张伟清, 林媚, 冯先桔, 等. 杨梅的营养保健价值及其加工技术进展[J]. 浙江柑橘, 2012(4): 26-30.
[3] ZHOU Shao-huan, FANG Zhong-xiang, LV Yuan, et al. Phenolics and antioxidant properties of bayberry (Myrica rubra Sieb. et Zucc.) pomace[J]. Food Chemistry, 2009, 112(2): 394-399.
[4] ZHENG Yi-xiong, CHEN Qing-jun, SUN Chong-de, et al. Effects of flavonoids-rich Chinese bayberry (Myrica rubra, Sieb. et Zucc.) pulp extracts on glucose consumption in human HepG2 cells[J]. Journal of Functional Foods, 2015, 14: 144-153.
[5] SUN Chong-de, ZHENG Yi-xiong, CHEN Qing-jun, et al. Purification and anti-tumour activity of cyanidin-3-O-glucoside from Chinese bayberry fruit[J]. Food Chemistry, 2012, 131(4): 1 287-1 294.
[6] JAGTAP U B, BAPAT V A. Wines from fruits other than grapes: Current status and future prospectus[J]. Food Bioscience, 2015, 9: 80-96.
[7] 刘永衡, 华惠敏, 吴桂君, 等. 果酒酵母选育及酵母对香气成分影响的研究进展[J]. 中国酿造, 2013, 32(10): 5-8.
[8] 赵祥杰, 陈卫东, 刘学铭, 等. 果酒酵母选育研究进展[J]. 酿酒, 2006, 33(1): 61-64.
[9] 李豪, 章霞, 张静, 等. 草莓果酒酵母菌的筛选、鉴定及耐受性研究[J]. 中国酿造, 2017, 36(2): 85-88.
[10] 苏凤贤, 张井, 郑晓杰. 人参果自然发酵醪中酵母菌种筛选及其发酵性能[J]. 食品科学, 2017, 38(4): 100-106.
[11] 左勇, 杨小龙, 傅彬, 等. 野生猕猴桃果酒酵母菌的筛选及鉴定[J]. 酿酒科技, 2015(11): 37-40.
[12] 杜晶, 余培斌, 苏琪, 等. 杨梅果酒酿造酵母的筛选、鉴定及耐受性研究[J]. 食品与发酵工业, 2015, 41(9): 34-39.
[13] 剧柠, 赵梅梅, 柯媛, 等. 枸杞果酒用非酿酒酵母的分离筛选及香气成分分析[J]. 食品与发酵工业, 2017(11): 125-131.
[14] 李锐, 冯奎, 吴婧, 等. 不同来源酿酒酵母对柑橘果酒香气成分的影响[J]. 食品科学, 2010, 31(17): 206-213.
[15] 胡晓冰, 王振伟. TTC法在筛选西瓜果酒酵母中的应用[J]. 酿酒科技, 2011(2): 69-70.
[16] 甘广东, 陆其刚, 冯海燕, 等. 酒醅中优势酵母菌的筛选及生理生化特性分析[J]. 酿酒, 2016, 43(1): 31-35.
[17] 郝瑶, 王陶, 李文, 等. 富硒猕猴桃果酒酵母的筛选及鉴定[J]. 食品科学, 2014, 35(21): 175-179.
[18] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB/T 15038—2006 葡萄酒、果酒通用分析方法[S]. 北京: 中国标准出版社, 2006.
[19] 孙婧超, 刘玉田, 赵玉平, 等. pH示差法测定蓝莓酒中花色苷条件的优化[J]. 中国酿造, 2011, 30(11): 171-174.
[20] 徐俊, 雍晓雨, 费文斌, 等. 基于TTC染色法的高活力酵母细胞定量筛选[J]. 食品与发酵工业, 2014, 40(7): 1-5.
[21] 徐本刚, 母应春, 陈虹文, 等. 贵州黑糯米酒曲优良酵母菌分离筛选与鉴定[J]. 中国酿造, 2012, 31(4): 62-67.
[22] 张陈云, 刘金福, 王娜, 等. 冬枣果酒酿造酵母的分离筛选研究[J]. 中国酿造, 2010, 29(8): 43-47.
[23] 杨鲁君, 蒋予箭, 李余动. 黄酒酵母优良抗逆菌株的筛选、鉴定及发酵特性研究[J]. 中国食品学报, 2013, 13(9): 71-77.
[24] 侍崇娟, 吕钰凤, 杜晶, 等. 杨梅酒发酵工艺及其风味变化[J]. 食品工业科技, 2015, 36(6): 166-170.
[25] 沈颖, 刘晓艳, 白卫东, 等. 果酒中有机酸及其对果酒作用的研究[J]. 中国酿造, 2012, 31(2): 29-32.
[26] 马勇, 图雅, 陈秀莉, 等. 分光光度法测定高浓度培养液下的产油酵母菌生长曲线[J]. 北方园艺, 2013(8): 116-118.
[27] 何贝, 王学东, 叶鹏, 等. 几种品牌酵母耐性生长曲线对比研究[J]. 中国酿造, 2014(12): 63-67.
[28] 阎贺静, 时月, 刘畅, 等. 玫瑰香干红葡萄酒自然发酵过程中优势酵母分离鉴定及其应用潜力分析[J]. 食品科学, 2017, 38(22): 117-124.
[29] 朱宝生, 刘功良, 白卫东, 等. 耐高糖酵母筛选及其高糖胁迫机制的研究进展[J]. 中国酿造, 2016, 35(6): 11-14.
[30] 王孝荣, 罗佳丽, 潘年龙, 等. 草莓果酒酿造工艺的优化及其香气成分分析[J]. 食品科学, 2014, 35(7): 196-201.
[31] 尤亮, 丁东栋, 崔志峰. 乙醇耐受性酿酒酵母菌株选育的研究进展[J]. 工业微生物, 2016, 46(3): 51-55.
[32] 张强, 郭元, 韩德明. 酿酒酵母乙醇耐受性的研究进展[J]. 化工进展, 2014, 33(1): 187-192.

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