Abstract
The extraction and identification and anti-fatigue activity of iridoid glycosides in walnut meal were studied. The walnut was extracted by ethanol reflux, extracted with ethyl acetate and n-butanol. Different solvent extracts were used to determine the anti-fatigue effect by mouse running test and weight-bearing swimming test. The extract with clear anti-fatigue effect was further separated by MCI and ODS reverse-phase column chromatography, and the obtained monomer compound was identified by NMR. The results showed that the extract of n-butanol from the walnut meal extract had the most obvious effect on the anti-fatigue effect of the mice. The part of the ODS column was eluted with 5% ethanol to obtain the compound II. The compound was identified as the iridoid glycoside. And can significantly improve the anti-fatigue ability of mice.
Publication Date
7-28-2019
First Page
172
Last Page
175,236
DOI
10.13652/j.issn.1003-5788.2019.07.033
Recommended Citation
Meng, JIN; Deping, XU; Ji, ZHAN; and Chen, CHEN
(2019)
"Extraction, identification and anti-fatigue activity of iridoid glycosides from walnut meal,"
Food and Machinery: Vol. 35:
Iss.
7, Article 33.
DOI: 10.13652/j.issn.1003-5788.2019.07.033
Available at:
https://www.ifoodmm.cn/journal/vol35/iss7/33
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