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Abstract

The effect of Rho18 treated with different methods on the removal of deoxynivalenol (DON) from wheat flour was determined by ELISA and HPLC-MS. Processing results showed that the different ways of rice root mildew Rho18 clearance to DON in wheat flour were all increased with the concentration increase with the extension of incubation time, as well as the high concentration of the treatment group. After 10 h incubation with the rest of the concentration of treatment group were significantly different, the cell walls of a high concentration of suspension and wheat 10 h incubation conditions to DON removal effect is best (51.62%). Three kinds of new substances with relative molecular weight of 277, 279 and 281 were produced by Rho18 and DON treated in different ways. The results showed that Rho18 of Rhizopus oryzae treated in different ways had certain scavenging effects on DON, and the effect was related to concentration and incubation time, and its scavenging mechanism was mainly the action of biological enzyme system.

Publication Date

9-28-2019

First Page

69

Last Page

73,146

DOI

10.13652/j.issn.1003-5788.2019.09.013

References

[1] 张琛, 许慧卿, 崔桂友,等. 纳豆芽孢杆菌Bacillus natto 16-1对小麦粉中呕吐毒素的脱毒机制[J]. 食品与机械, 2019, 35(3): 8-14.
[2] 朱金林. 呕吐毒素最新研究进展及发展趋势[J]. 国外畜牧学: 猪与禽, 2013, 33(12): 102-104.
[3] BINDER J, HORVATH E M, SCHATZMAYR G, et al. Screening for deoxynivalenol-detoxifying anaerobic rumen microorganisms[J]. Cereal Research Communications, 1997, 25(3): 343-346.
[4] HE P, YOUNG L G, FORSBERG C. Microbial transformation of deoxynivalenol(vomitoxin)[J]. Applied and Environmental Microbiology, 1993, 58(12): 3 857-3 863.
[5] LONDOO-HERNNDEZ L, RAMREZ-TORO C, RUIZ H A, et al. Rhizopus oryzae - Ancient microbial resource with importance in modern food industry[J]. Food Microbiol, 2017, 257: 110-127.
[6] 吴寒. 米根霉和乳酸菌混合固态发酵对燕麦营养及功能性的影响[D]. 南京: 南京农业大学, 2015: 16-17.
[7] DENARDI-SOUZA T, LUZ C, MAES J, et al. Antifungal effect of phenolic extract of fermented rice bran with Rhizopus oryzae and its potential use in loaf bread shelf life extension[J]. Food Agric, 2018, 98(13): 5 011-5 018.
[8] 张立阳, 赵雪娇, 刘帅, 等. 食品及饲料中黄曲霉毒素生物脱毒的研究进展[J]. 动物营养学报, 2019, 31(2): 521-529.
[9] 赵俊廷. NJA-1菌产环氧化物水解酶条件的优化及对脱氧雪腐镰刀菌烯醇生物转化的研究[D]. 南京: 南京农业大学, 2008: 38-40.
[10] 许娇娇, 黄百芬, 蔡增轩, 等. 面粉中脱氧雪腐镰刀菌烯醇标准物质的研制[J]. 中国食品卫生杂志, 2016, 28(3): 291-294.
[11] 吴娱, 王开萍, 唐正江, 等. 米曲霉Aspergillus oryzae As-W.6对脱氧雪腐镰刀菌烯醇的降解效果[J]. 食品科学, 2016, 37(17): 185-189.
[12] JNOS V, ZSANETT P, KATALIN T, et al. Degradation of ochratoxin A and other mycotoxins by Rhizopus isolates[J]. International Journal of Food Microbiology, 2005, 99(3): 321-328.
[13] MARLEEN P, JANINE W, SUSANNE K, et al. Studies on the bioavailability of deoxynivalenol (DON) and DON sulfonate (DONS) 1, 2, and 3 in pigs fed with sodium sulfite-treated DON-contaminated maize[J]. Toxins, 2015, 7(11): 4 622-4 644.
[14] 耿海荣, 张晨曦, 赵月菊, 等. 一株高效降解玉米赤霉烯酮的耐酸耐高温枯草芽孢杆菌的研究[J]. 核农学报, 2019(7): 1 399-1 407.
[15] PALOMO J M, MUOZ G, FERNNDEZ-LORENTE G, et al. Modulation of mucormiehei lipase properties via directed immobilization on different hetero-functional epoxy resins: Hydrolytic resolution of (R, S)-2-butyroyl-2-phenylacetic acid[J]. Journal of Molecular Catalysis B Enzymatic, 2003, 21(4): 201-210.
[16] POPPENBERGER B, BERTHILLER F, LUCYSHYN D, et al. Detoxification of the fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from arabidopsis thaliana[J]. Journal of Biological Chemistry, 2003, 278(48): 47 905-47 914.
[17] 魏蒙月, 张赟彬, 吴酉芝, 等. 香辛料精油-β-环糊精微胶囊的物理特征及对酱牛肉熏蒸保鲜的效果研究[J]. 中国食品添加剂, 2019(4): 127-133.
[18] 曹慧英, 伍松陵, 孙长坡. 呕吐毒素(DON)生物合成和降解研究进展[J]. 中国粮油学报, 2013, 28(5): 116-123.
[19] 胡晓清, 褚召娟, 张继祥, 等. 酵母生物脱毒的研究进展[J]. 现代食品科技, 2017, 33(12): 272-277.
[20] 陈世年. 从米根霉细胞壁寻找天然壳聚糖的研究Ⅳ[J]. 华侨大学学报: 自然科学版, 1997(1): 80-82.
[21] 李子琪, 孟倩, 孙凤清, 等. 壳聚糖及其衍生物脱除贝类中重金属的机理及应用研究进展[J]. 食品与机械, 2016, 32(2): 188-192.
[22] 李冠龙, 刘晓兰, 波利特. β-半乳糖苷酶的固定化及其在乳清行业中的应用[J]. 食品科技, 2019, 44(2): 264-269.
[23] MENG Qin, LU De-wei. Biosorption of Ni2+ and Fe3+ by fungal cell wall sacchrides[J]. Chinese Journal of Chemical Engineering, 2000(2): 123-127.
[24] 张亚娟. 黑根霉菌对重金属废水的生物吸附及其固定化技术[J]. 环保科技, 2009, 15(3): 30-34.
[25] SALLEH A B, MUSANI R, BASRI M, et al. Extra and intracellular lipase from a thermophile Rhizopus oryzae and factors affecting their production[J]. Canadian Journal of Microbiology, 1993, 39(10): 978-981.
[26] 于大海. 脂肪酶拆分环氧丙醇和2-辛醇[D]. 吉林: 吉林大学, 2008: 37-38.
[27] 乐华爱, 谢玉梅, 张树政, 等. 根霉的研究Ⅳ: 根霉淀粉葡萄糖苷酶的研究[J]. 微生物学报, 1966, 12(2): 187-193.
[28] 范凯, 王栋, 徐岩. 华根霉液态培养生产脂肪酶不同形态菌体的转录组差异分析[J]. 微生物学通报, 2018, 45(1): 29-41.
[29] 窦晓, 杨建刚, 马莹莹, 等. 华根霉在酿酒中的研究进展[J]. 食品与发酵工业, 2015, 41(4): 246-250.

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