Study on identification of fritillariae cirrhosae bulbus by improved alkali pyrolysis

Authors

CHEN Yuxiu, Hunan Food and Drug Vocational College, Changsha, Hunan 410208, China
PENG Biwu, Hunan Food and Drug Vocational College, Changsha, Hunan 410208, China
LI Yuyan, Hunan Food and Drug Vocational College, Changsha, Hunan 410208, China

Abstract

The DNA of Fritillariae cirrhosae bulbus was extracted by alkali lysis. The effects of different proportion of lysate on DNA quality and electrophoretic success rate were investigated, which compared with the current methods. Results, The alkaline lysis buffer had the best effect on DNA extraction, which was made of 200 mmol/L NaCl, 5 mmol/L Mg₂Cl, 1% Triton X-100, 1% PVP, 1% SDS, 2 mmol/L EDTA and 0.1 mol/L Tris-HCl. The DNA electrophoretic bands extracted by this alkali lysate were in accordance with the current method. The method can save 15~20 minutes, and the cost of single sample testing can be reduced to 0.01 yuan. This method was used to identify 25 samples of Fritillariae cirrhosae bulbus on the market. The results showed that Fritillariae cirrhosae bulbus could be identified quickly.

Publication Date

9-28-2019

First Page

94

Last Page

97

DOI

10.13652/j.issn.1003-5788.2019.09.018

Recommended Citation

Yuxiu, CHEN; Biwu, PENG; and Yuyan, LI (2019) "Study on identification of fritillariae cirrhosae bulbus by improved alkali pyrolysis," Food and Machinery: Vol. 35: Iss. 9, Article 18.
DOI: 10.13652/j.issn.1003-5788.2019.09.018
Available at: https://www.ifoodmm.cn/journal/vol35/iss9/18

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