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Abstract

Five strains were isolated and purified from moldy cigarettes, and were identified by fungal morphology and microscopy. It was found that there were 2 strains of Aspergillus, 1 strain of Penicillium, 1 strain of Alternaria, and 1 strain of Cladosporium. Through the 18S rDNA-ITS sequence analysis and the construction of phylogenetic tree, the mildew microorganism of cigarettes were identified as five species, such as Aspergillus sydowii, Aspergillus flavus, Cladosporiumtenuissimum, Penicilliumdigitatum and Alternaria. It was concluded that Aspergillus flavus was the main strain causing mildew in cigarette products by the mildew experiment.

Publication Date

9-28-2019

First Page

98

Last Page

101

DOI

10.13652/j.issn.1003-5788.2019.09.019

References

[1] 梁栋. 烟草行业实时精准营销的实践策略分析与阐述[J]. 中小企业管理与科技, 2018(34): 9-10.
[2] 向东红. 川渝地区仓储片烟主要霉变微生物及防霉技术研究[D]. 青岛: 中国农业科学院, 2010: 28.
[3] 晏卫红, 黄思良, 朱桂宁, 等. 广西仓储烟叶霉变微生物的分类鉴定[J]. 烟草科技, 2008(2): 50-56.
[4] 曾婷英, 顾钢, 张绍升. 烘烤期烟叶霉烂病的病原鉴定[J]. 中国烟草学报, 2014(4): 65-68.
[5] 邱小梅. 环境温湿度对烟草物料水份的影响[J]. 福建质量管理, 2012(5): 61.
[6] 李跃锋, 王锐亮, 陈河祥, 等. 烟支霉变微生物的鉴定及其生物学特性分析[J]. 烟草科技, 2017, 50(6): 9-15.
[7] 王儒. 烟支霉变微生物的鉴定及生物特性研究[D]. 广州: 华南理工大学, 2015: 42.
[8] 谢欣. 烟支霉变微生物的分离与霉变成因研究[D]. 广州: 华南理工大学, 2014: 34-35.
[9] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979: 1-781.
[10] 冯长海. 改良透明胶带法在丝状真菌形态观察中的应用[J]. 临床检验杂志, 2017, 35(10): 32-33.
[11] LI Zai-xin, LEI Yun-ling, LIU Da-yu, et al. Molecular cloning and sequence analysis of partial 18S rDNA of mold in Koji[J]. Food Science, 2009, 30(1): 211-213.
[12] 杨红红, 陈国刚, 刘娅, 等. 桶装胀罐番茄酱中腐败菌分离纯化及鉴定研究[J]. 食品工业科技, 2014, 35(1): 164-167.
[13] KOICHIRO T, MASATOSHI N, SUDHIR K. Prospects for inferring very large phylogenies by using the neighbor-joining method[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(30): 11 030-11 035.
[14] 张耀东. 几种石莼目大型海洋绿藻的系统发育分析[D]. 苏州: 苏州大学, 2008: 17.
[15] 李春艳, 赵高岭, 叶红朝, 等. 河南烟叶仓储期优势霉菌的分离鉴定及防霉剂的筛选[J]. 烟草科技, 2011(7): 66-70.

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