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Abstract

Single factor combined with orthogonal test was employed to optimize the best technology for fermentation of tobacco leaves by Lactobacillus plantarum in this study. The chemical components, aroma substances and sensory evaluation of fermented tobacco leaves were analyzed. The optimal fermentation technology of tobacco leaves by L. plantarum was achieved when the addition of L. plantarum, glucose syrup and soy protein peptide was 0.80‰, 5.0‰, and 0.05‰ respectively, with fermentation for 8 days. The starch content of tobacco leaves decreased to the most significantly, and 9 kinds of new volatile aroma substances were detected. The content of nicotine decreased by 5.58%. Fermented tobacco leaves from different regions was compared, and it was found that the addition of L. plantarum, carbon and nitrogen sources could effectively improve the smoking quality of tobacco leaves from various regions. The content of starch decreased significantly while that of soluble total sugar and reducing sugar increased significantly (P<0.05), among which the tobacco leaves from Chongqing changed to the most extent. The total alkaloids and total nitrogen content of tobacco leaves decreased slightly while the sensory evaluation was enhanced significantly (P<0.05), among which the tobacco leaves from Guizhou improved to the most extent by 1.875 points.

Publication Date

5-28-2020

First Page

199

Last Page

204,226

DOI

10.13652/j.issn.1003-5788.2020.05.037

References

[1] 王颖.固态发酵卷烟叶组理化和香气成分研究[D].咸阳:西北农林科技大学,2013:1-2.
[2] 冯颖杰,袁岐山,杨宗灿,等.一株鞘氨醇单胞菌对复烤后烟叶多酚物质的降解作用[J].中国烟草学报,2019,25(1):23-28.
[3] 杨娟,米其利,熊文,等.食源性乳酸菌在改善烟叶品质中的应用[J].浙江农业学报,2018,30(5):854-862.
[4] 全铭沁,董惠忠,沙云菲,等.黑曲霉发酵液改善上部烟叶风味品质的研究[J].中国酿造,2019,38(3):170-176.
[5] 谷月.发酵法制备口用型烟草制品烟丝及其品质变化研究[D].广州:华南理工大学,2016.
[6] 王怀珠,杨焕文,郭红英,等.淀粉类酶降解鲜烟叶中淀粉的研究[J].中国烟草科学,2005,26(2):37-39.
[7] 董维杰,张忠锋,窦玉青,等.烤烟烟叶淀粉含量影响因素及其与烟叶质量关系研究进展[J].广东农业科学,2015,42(8):11-16.
[8] 王红刚,董维杰,窦玉青,等.烤烟烟叶淀粉含量与其感官质量的相关性研究[J].西南农业学报,2017,30(7):1 533-1 537.
[9] 刘谋盛,王平艳,杨亚玲,等.活性物质降解烟叶中淀粉的工艺研究[J].精细化工,2007,24(9):881-884.
[10] 沙云菲,董惠忠,张耀,等.降解上部烟叶大分子物质的复合酶配制与条件优化[J].中国烟草科学,2018,39(3):83-91,100.
[11] 胡志忠,许春平,姜宇,等.利用产香酵母发酵技术改善烟叶品质[J].食品与机械,2018,34(11):200-204.
[12] 何其芳,李荣华,郭培国,等.烟叶中淀粉含量测定方法的比较[J].现代食品科技,2012,28(2):229-232.
[13] BUJALANCE C,JIMNEZ-VALERA M,MORENO E,et al.A selective differential medium for Lactobacillus plantarum[J].Journal of Microbiological Methods,2006,66(3):572-575.
[14] 李冬华.直投式植物乳杆菌发酵剂生产工艺及应用的研究[D].广州:华南理工大学,2010:8-9.
[15] 曹承旭,郭晶晶,乌日娜,等.植物乳杆菌的生理功能和组学研究进展[J].乳业科学与技术,2018,41(1):33-39.
[16] DI GIACOMO M,PAOLINO M,SILVESTRO D,et al.Microbial community structure and dynamics of dark fire-cured tobacco fermentation[J].Applied & Environmental Microbiology,2007,73(3):825.
[17] 李秀妮.烟叶微生物及其在烟叶发酵和醇化中的作用研究进展[J].微生物学通报,2019,46(6):1 520-1 529.
[18] 王勇,王行,贺广生,等.耐高温产淀粉酶芽孢杆菌在烟叶烘烤中降解淀粉的应用研究[J].中国烟草学报,2017,23(4):56-63.
[19] 郭华诚,张月华,李阳光,等.烟丝挥发性香味物质与卷烟感官质量的相关性研究[J].食品与机械,2019,35(1):209-212.
[20] 张蕊.不同产区烤烟淀粉含量分布特点及与烟味香型和感官质量的关系[D].郑州:河南农业大学,2011:19-20.
[21] 陈颐,杨虹琦,杨佳玫,等.不同香型烤烟香气前体物特征及其对感官评吸的影响[J].云南农业大学学报,2016,31(3):489-497.

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