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Abstract

Explore the analgesic effects and active components of Lycopodium japonicum Thunb. Ethanol extraction, water extraction and ethanol precipitation were used to obtain different extracts of Lycopodium japonicum Thunb. Using acetic acid writhing and hot plate models, using mouse writhing reaction time and licking rear feet time as indicators, the analgesic components were screened and analyzed by nuclear magnetism. The water extract of Lycopodium japonicum Thunb has analgesic effect. After further analysis and screening, it is found that the analgesic components of Lycopodium japonicum Thunb are polysaccharides. A single component polysaccharide is obtained from the crude polysaccharides, and its structure is analyzed by nuclear magnetic field. The main chain of the polysaccharide is composed of (1→4)-β-glucose, with side chains attached to position 2 and acetyl groups attached to position 6.

Publication Date

3-28-2021

First Page

157

Last Page

161

DOI

10.13652/j.issn.1003-5788.2021.03.029

References

[1] CHEN Ye-gao, YANG Qian, ZHANG Yan. Lycopodium japonicum: A comprehensive review on its phytochemicals and biological activities[J]. Arabian Journal of Chemistry, 2020, 13(5): 5 438-5 450.
[2] HE Juan, CHEN Xuan-qin, LI Ming-ming, et al. Lycojapodine A, a novel alkaloid from Lycopodium japonicum[J]. Organic Letters, 2009, 11(6): 1 397-1 400.
[3] 杨申明, 范树国, 王振吉, 等. 乙醇回流法提取伸筋草总黄酮工艺及其体外抗氧化活性[J]. 北方园艺, 2016(6): 116-120.
[4] 曾元儿, 叶木荣, 徐晖. 伸筋草不同提取部位抗炎镇痛药理实验研究[J]. 时珍国医国药, 1999(9): 641-642.
[5] 李墨娇, 刘杰, 张玉波, 等. 伸筋草的化学成分研究[J]. 中草药, 2015, 46(1): 33-37.
[6] 刘巧洲, 郭静, 石磊, 等. 眼镜蛇毒因子抑制小鼠疼痛反应[J]. 中国药理学通报, 2019, 35(5): 739-740.
[7] 周殿儒, 周游, 魏谭军, 等. 理气散结颗粒理气调血,散结止痛的药效学作用[J]. 中成药, 2020, 42(2): 484-488.
[8] 闫旭宇, 李玲. 水提醇沉法提取薏米多糖及其对羟自由基的清除作用[J]. 食品研究与开发, 2019, 40(18): 1-5.
[9] 侯云飞, 徐德平. 仙灵脾改善小鼠睡眠功效成分的分离鉴定[J]. 食品与机械, 2019, 35(12): 146-150.
[10] 韦卓纯, 黄卫娟, 姚志红, 等. 肿节风高效薄层色谱鉴别[J]. 中成药, 2019, 41(7): 1 728-1 731.
[11] 陈奇. 中药药理研究方法学[M]. 北京: 人民卫生出版社, 2011: 427-428.
[12] 陈小野, 张宇鹏, 蒋小丽, 等. 热板法、醋酸扭体法疼痛模型寒、热属性的初步研究[J]. 浙江中医学院学报, 2005, 29(4): 46-51.
[13] XI Xiong-gang, WEI Xin-lin, WANG Yuan-feng, et al. Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method[J]. Archives of Biological Sciences, 2010, 62(3): 671-678.
[14] 刘晓飞, 张宇, 王薇, 等. 发芽糙米中粗多糖的纯化及分子量测定[J]. 食品工业科技, 2019, 40(2): 25-30.
[15] 安超, 薛文娇, 马赛箭, 等. 高效凝胶色谱法同时测定普鲁兰多糖生物合成过程中分子量和含量[J]. 食品研究与开发, 2018, 39(7): 143-148.
[16] 钱明辉, 达热卓玛, 徐德平. 荞麦蜂花粉多糖A的分离及其降血糖活性[J]. 食品与生物技术学报, 2019, 38(6): 80-85.
[17] 叶盛英, 杨本明, 杜欣, 等. 中药伸筋草研究概况[J]. 药学实践杂志, 2009, 27(1): 18-20.
[18] 陆怡, 朱元章, 朱国福, 等. 中药镇痛机理研究概述[J]. 世界中医药, 2015(4): 629-632.
[19] 潘景芝, 金莎, 刘雅婧, 等. 刺五加多糖镇痛抗炎作用及其免疫调节机制的研究[J]. 上海中医药杂志, 2019, 53(10): 74-82.

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