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Abstract

Gas chromatography-mass spectrometry (GC-MS) was used to analyze fatty acid profile in metabolites and cells of five strains of Bifidobacterium. The results showed that the fatty acid content of different Bifidobacterium was significantly different among the metabolites, and there were some differences among the cells. Differences of fatty acid were also found among the same Bifidobacteriium strains derived from different sources. Methyl palmitate (C16:0), methyl lignocerate (C24:0), and methyl stearate (C18:0) were the main fatty acids in metabolites and cells of the five strains. Among them, the C16:0 was significantly different (P<0.05), and the content ratio of C16:0 to C18:0 was between 2.607 and 3.120; the content ratio of acetic acid to lactic acid was between 2.085 and 2.295. Compared with the metabolites, methyl erucate (C22:1n-9), methyl nervonate (C24:1n-14), methyl 5, 8, 11, 14, 17-eicosapentaenoate (C20:5n-3) were not detected in the cells of the five strains.

Publication Date

4-28-2021

First Page

19

Last Page

25

DOI

10.13652/j.issn.1003-5788.2021.04.004

References

[1] LU Nong-hua, NIE Yong-zhan, XU Wen-ting, et al. The crosstalk between gut microbiota and obesity and related metabolic disorders[J]. Future Microbiology, 2016, 11(6): 825-836.
[2] KASSAA I A. The antiviral activity of probiotic metabolites[M]. California: Springer, Cham, 2017: 84-94.
[3] FIROZ A, HISHBAM A N, FAHAD AA, et al. Antiviral effects of probiotic metabolites on COVID-19[J/OL]. Journal of Biomolecular Structure and Dynamics. [2020-11-05]. https://doi.org/10.1080/07391102.2020.1775123.
[4] 附俊杰, 李丽, 刘军, 等. 丁酸梭菌及其代谢产物在食品加工中的应用[J]. 食品与发酵工业, 2020, 46(13): 293-298.
[5] 吕锡斌, 何腊平, 张汝娇, 等. 双歧杆菌生理功能研究进展[J]. 食品工业科技, 2013, 34(16): 353-358.
[6] WANG Huan, GONG Jing, WANG Wen-feng, et al. Are there any different effects of Bifidobacterium, Lactobacillus and Streptococcus on intestinal sensation, barrier function and intestinal immunity in PI-IBS mouse model[J]. PloS one, 2014, 9(3): e90153.
[7] KWAK M J, KWON S K, YOON J K, et al. Evolutionary architecture of the infant-adapted group of Bifidobacterium species associated with the probiotic function[J]. Systematic & Applied Microbiology, 2016, 39(7): 429-439.
[8] 陈银基, 鞠兴荣, 周光宏. 饱和脂肪酸分类与生理功能[J]. 中国油脂, 2008(3): 35-39.
[9] LEVI L, WANG Z, DOUD M K, et al. Saturated fatty-acids regulate retinoic acid signaling and suppress tumorigenesis by targeting fatty-acid-binding protein 5[J]. Nature Communications, 2015, 6: 8 794.
[10] ZHANG Rui-nan, QIN Pan, ZHANG Zheng, et al. Saturated fatty acid inhibits viral replication in chronic hepatitis B virus infection with nonalcoholic fatty liver disease by toll-like receptor 4-mediated innate immune response[J]. Hepatitis Monthly, 2015, 15(5): e27909.
[11] 王炜, 张伟敏. 单不饱和脂肪酸的功能特性[J]. 中国食物与营养, 2005(4): 44-46.
[12] 刘跟生, 徐贵发. 单不饱和脂肪酸对心血管的保护作用[J]. 卫生研究, 2006(3): 104-106.
[13] LUCHTMAN D W, SONG C. Cognitive enhancement by omega-3 fatty acids from child-hood to old age: Findings from animal and clinical studies[J]. Neuropharmacology, 2013, 64: 550-565.
[14] JANSSEN C I F, KILIAAN A J. Long-chain polyunsaturated fatty acids (LCPUFA) from genesis to senescence: The influence of LCPUFA on neural development, aging, and neurodegeneration[J]. Progress in Lipid Research, 2014, 53: 1-17.
[15] 王萍, 张银波, 江木兰. 多不饱和脂肪酸的研究进展[J]. 中国油脂, 2008, 33(12): 42-46.
[16] VOORHEES K J, JENSEN K R, MCALPIN C R, et al. Modified MALDI MS fatty acid profiling for bacterial identification[J]. Journal of Mass Spectrometry, 2013, 48(7): 850-855.
[17] SHIM S K, SHIN Y K, CHOI E N, et al. Analysis of cellular fatty acids inorientia tsutsugamushi as taxonomic markers[J]. Microbiology & Immunology, 2013, 49(4): 343-347.
[18] INES C S, JONATHAN S, WOOSIK C, et al. Analysis of bacterial FAMEs using gas chromatography-vacuum ultraviolet spectroscopy for the identification and discrimination of bacteria[J]. Talanta, 2018, 182: 536-543.
[19] 蔡静静, 徐晓裕, 张艳, 等. 新疆维吾尔族肠道中高产胞外多糖双歧杆菌的筛选及其抗氧化活性研究[J]. 食品科学, 2020, 41(8): 144-151.
[20] KENTA S, KEITA N, HIROKI M, et al. Adhesion properties of a putative polymorphic fimbrial subunit protein from Bifidobacterium longum subsp. longum[J]. Bioscience of Microbiota, 2016, 35(1): 19-27.
[21] VEERKAMP J H. Fatty acid composition of Bifidobacterium and Lactobacillus strains[J]. Journal of Bacteriology, 1971, 108(2): 861-867.
[22] 许强, 王郡甫, 许晓群, 等. 气相色谱法测定双歧杆菌代谢产物中乙酸和乳酸[J]. 理化检验(化学分册), 2016, 52(8): 959-961.
[23] 徐敏, 王静, 柴子涵, 等. 海洋细菌脂肪酸的气相色谱分析[J]. 海洋科学, 2013, 37(2): 76-83.
[24] 仲玉备, 陈历俊, 赵军英, 等. 泌乳期母乳脂肪酸变化及其多不饱和脂肪酸影响因素[J]. 食品科学, 2019, 40(4): 237-243.
[25] 中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准 食品微生物学检验 双歧杆菌检验: GB 4789.34—2016[S]. 北京: 中国标准出版社, 2016: 9-10.
[26] 田芬, 陈俊亮, 霍贵成. 嗜酸乳杆菌和双歧杆菌的主代谢产物分析[J]. 中国食品学报, 2013, 13(6): 220-226.
[27] 陆春波, 毛银, 李国辉, 等. 植物乳杆菌DY6主要抑菌代谢物的分析和鉴定[J]. 微生物学通报, 2019, 46(9): 2 258-2 271
[28] LEONARDO A, HISAKAZU K, HIKARU I, et al. Transcriptomic analysis of the innate antiviral immune response in porcine intestinal epithelial cells: Influence of immunobiotic lactobacilli[J]. Frontiers in Immunology, 2017, 8: 57.
[29] DONG Li, ZHONG Xiang, HE Jin-tian, et al. Supplementa-tion of tributyrin improves the growth and intestinal digestive and barrier functions in intrauterine growth-restricted piglets[J]. Clinical Nutrition, 2016, 35(2): 399-407.
[30] 岳莹雪, 王玉琦, 闫芬芬, 等. 丁酸的生产方法及在肠道中的生理功能研究进展[J]. 食品工业科技, 2019, 40(15): 339-344.
[31] MAEKAWA S, TAKADA S, NAMBBBU H, et al. Linoleic acid improves assembly of the CII subunit and CIII2/CIV complex of the mitochondrial oxidative phosphorylation system in heart failure[J]. Cell Communication and Signaling, 2019, 17(1): 30-41.
[32] MICHIEL S L, BOUDEWIJN R, NORTJE V D B, et al. Genetically determined prospect to become long-lived is associated with less abdominal fat and in particular less abdominal visceral fat in men[J]. Age and Ageing: The Journal of the British Geriatrics Society and the British Society for Research on Ageing, 2015, 44(4): 713-717.
[33] YARON S, SHACHAR D, ABRAMAS L, et al. Effect of high β-palmitate content in infant formula on the intestinal microbiota of term infants[J]. Journal of Pediatric Gastroenterology and Nutrition, 2013, 56(4): 376-381.
[34] KIM S A, RHEE M S. Highly enhanced bactericidal effects of medium chain fatty acids (caprylic, capric, and lauric acid) combined with edible plant essential oils (carvacrol, eugenol, β-resorcylic acid, trans-cinnamaldehyde, thymol, and vanillin) against Escherichia coli O157:H7[J]. Food Control, 2016, 60: 447-454.
[35] 刘波, 王阶平, 刘国红, 等. 芽胞杆菌脂肪酸组学[M]. 北京: 科学出版社, 2019: 1 434.
[36] SEVERINE L, SEVERINE C R, RAPHAELLE T M, et al. Membrane fatty acid composition and fluidity are involved in the resistance to freezing of Lactobacillus buchneri R1102 and Bifidobacterium longum R0175[J]. Microbial Biotechnology, 2015, 8(2): 311-318.
[37] FLORENCE A C R, OLIVEIRA M N D, DELILE A, et al. Survival of Bifidobacterium strains in organic fermented milk is improved as a result of membrane fatty acid composition[J]. International Dairy Journal, 2016, 61: 1-9.
[38] COSTA M S, ALBUQUERQUE L, NORBE M F, et al. The identification of fatty acids in bacteria[J]. Methods in Microbiology, 2011, 38: 183-196.

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