Fluorescence aptasensor detection of chloramphenicol based on MnO₂ nanosheet and Exo-I

Authors

MA Peng-fei, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
QI Shuo, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
LU Yan, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
ZHOU You, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
WANG Zhou-ping, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

Abstract

In order to develop rapid detection method for chloramphenicol in food, in this study, a novel chloramphenicol aptasensor was constructed based on fluorescence quenching ability of MnO₂ nanosheet and fluorescence amplification ability of Exo-I digestion. Results showed that under optimal fluorescence detection conditions of 50 nmol/L concentration of aptamer, 0.05 mg/mL concentration of MnO₂ nanosheet, 0.4 U/μL concentration of Exo-I and Exo-I digestion time of 50 minutes, and the fluorescence intensity had a good linear relationship with the concentration of chloramphenicol (R²=0.995). The linear range of detection was 0.1~80.0 nmol/L with the detection limit of 0.08 nmol/L. The constructed detection approach had the advantages of simple operation and high sensitivity, and could realize accurate detection of chloramphenicol in food samples.

Publication Date

4-28-2021

First Page

53

Last Page

57

DOI

10.13652/j.issn.1003-5788.2021.04.009

Recommended Citation

Peng-fei, MA; Shuo, QI; Yan, LU; You, ZHOU; and Zhou-ping, WANG (2021) "Fluorescence aptasensor detection of chloramphenicol based on MnO₂ nanosheet and Exo-I," Food and Machinery: Vol. 37: Iss. 4, Article 9.
DOI: 10.13652/j.issn.1003-5788.2021.04.009
Available at: https://www.ifoodmm.cn/journal/vol37/iss4/9

References

[1] AMIR S, SHAWM M, ROBERT L, et al. Aquaculture practices and potential human health risks: Current knowledge and future priorities[J]. Environment International, 2008, 34: 1 215-1 226.
[2] DU Xin-jun, ZHOU Xiao-nan, WANG Shuo, et al. Development of an immunoassay for chloramphenicol based on the preparation of a specific single-chain variable fragment antibody[J]. Journal of Agriculture and Food Chemistry, 2016, 64: 2 971-2 979.
[3] ZHU Yu-han, LIU Xin, ZHANG Jing-dong, et al. A cathodic photovoltammetric sensor for chloramphenicol based on BiOI and graphene nanocomposites[J]. Sensors and Actuators B: Chemical, 2019, 284: 505-513.
[4] SANAZ P, JAYTRY M, FREDDY D, et al. Aptasensing of chloramphenicol in the presence of its analogues: Reaching the maximum residue limit[J]. Analytical Chemistry, 2012, 84: 6 753-6 758.
[5] JAYTRY M, WOUTER H, JOHAN R, et al. In vitro selection and characterization of DNA aptamers recognizing chloramphenicol[J]. Journal of Biotechnology, 2011, 155: 361-369.
[6] FADI A, GHISLAIN G, WALTER H, et al. Accurate quantitation and analysis of nitrofuran metabolites, chloramphenicol, and florfenicol in seafood by ultrahigh-performance liquid chromatography-tandem mass spectrometry: Method validation and regulatory samples[J]. Journal of Agriculture and Food Chemistry, 2018, 66: 5 018-5 030.
[7] BYZOVA N, ZVEREVA E, EREMIN S, et al. Rapid pretreatment-free immunochromatographic assay of chloramphenicol in milk[J]. Talanta, 2010, 81: 843-848.
[8] VICTORIA S, ABUZAR K, KENNETH G, et al. Fast extraction of amphenicols residues from raw milk using novel fabric phase sorptive extraction followed by high-performance liquid chromatography-diode array detection[J]. Analytica Chimica Acta, 2015, 855: 41-50.
[9] YAN Li, MAO Wei, DING Shi-jia, et al. A simple and sensitive electrochemical aptasensor for determination of Chloramphenicol in honey based on target-induced strand release[J]. Journal of Electroanalytical Chemistry, 2012, 687: 89-94.
[10] YANG Qian, ZHOU You, GAN Ning, et al. A two dimensional metaleorganic framework nanosheets-based fluorescence resonance energy transfer aptasensor with circular strand-replacement DNA polymerization target-triggered amplification strategy for homogenous detection of antibiotics[J]. Analytica Chimica Acta, 2018, 1 020: 1-8.
[11] YAN Chao, ZHANG Jing, CHEN Wei, et al. Aptamer-mediated colorimetric method for rapid and sensitive detection of chloramphenicol in food[J]. Food Chemistry, 2018, 260: 208-212.
[12] CHEN Ai-liang, YANG Shu-ming. Replacing antibodies with aptamers in lateral flow immunoassay[J]. Biosensors and Bioelectronics, 2015, 71: 230-242.
[13] YU Zi-qiang, ZHAO Xiao-hong, WANG Zhi, et al. Aptamers in hematological malignancies and their potential therapeutic implications[J]. Critical Reviews in Oncology/Hematology, 2016, 106: 108-117.
[14] TOH S, CITARTAN M, SUBAH C, et al. Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay[J]. Biosensors and Bioelectronics, 2015, 64: 392-403.
[15] MERAL Y, NAIMAT U, HIKMET B. Trends in aptamer selection methods and Applications[J]. Analyst, 2015, 140: 5 379-5 399.
[16] ZHANG Zi-jie, LIU Jue-wen. New insights into a classic aptamer: Binding sites, cooperativity and more sensitive adenosine detection[J]. Nucleic Acids Research, 2017, 45(13): 7 593-7 601.
[17] MATIC K, PETER P, HISAE T, et al. Thrombin binding aptamer G-quadruplex stabilized by pyrene-modified nucleotides[J]. Nucleic Acids Research, 2020, 48(7): 3 975-3 986.
[18] ZHU Chao, YANG Ge, QU Feng, et al. Evolution of multi-functional capillary electrophoresis for high-efficiency selection of aptamers[J]. Biotechnology Advances, 2019, 37: 107232.
[19] 赵旭, 乐琳, 王周平, 等. 基于核酸适配体的镉离子可视化检测方法[J]. 食品与机械, 2018, 34(6): 35-38.
[20] 许宙, 唐瑶, 程云辉, 等. 基于磁性纳米材料构建酶联增敏生物传感器检测双酚Ａ[J]. 食品与机械, 2017, 33(10): 47-51.
[21] LI Na, DIAO Wei, ZHANG Ting-ting, et al. MnO₂-modified persistent luminescence nanoparticles for detection and imaging of glutathione in living cells and in vivo[J]. Chemistry A European Journal, 2014, 20: 16 488-16 491.
[22] MENG Hong-min, JIN Zhen, YU Ru-qin, et al. Activatable two-photon fluorescence nanoprobe for bioimaging of glutathione in living cells and tissues[J]. Analytical Chemistry, 2014, 86(24): 12 321-12 326.
[23] MENG Hong-min, LU Li-ming, TAN Wei-hong, et al. Multiple functional nanoprobe for contrast-enhanced bimodal cellular imaging and targeted therapy[J]. Analytical Chemistry, 2015, 87(8): 4 448-4 454.
[24] WANG Chun-xia, YU Ping, MAO Lan-qun, et al. MnO₂ nanosheets based fluorescent sensing platform with organic dyes as a probe with excellent analytical properties[J]. Analyst, 140: 4 021-4 029.
[25] HAN Lie, ZHANG Hai-jiao, LI feng, et al. Protein-directed metal oxide nanoflakes with tandem enzyme-like characteristics: Colorimetric glucose sensing based on one-pot enzyme-free cascade catalysis[J]. Advanced Functional Materia, 2018, 28: 1800018.