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Abstract

Objective:This study aimed to optimize the extraction technology of total flavonoids from green tea with Deep Eutectic Solvents (DESs) and evaluate the antioxidant activity of total flavonoids extracted from green tea under optimal conditions.Methods:The extraction rate of total flavonoids was taken as the index, with the ratio of liquid to solid, extraction temperature and extraction time taken as the influencing factors, and the extraction process parameters were optimized by orthogonal test, followed by the antioxidant activity evaluating with DPPH free radical inhibition.Results:The optimal extraction conditions for extraction of total flavonoids from green tea with DESs were 80% acetylcholine-lactic acid solution (acetylcholine∶lactic acid =1∶1), with ratio of liquid to solid 30∶1 (mL/g), extraction at 90 ℃ for 75 min. Under the control of these conditions, the extraction yield of total flavonoids was 1.84%, and the concentra-tion of total flavonoids was 65.8 mg/mL. The research on antioxidant activity showed that in a certain concentration range, the antioxidant activity of total flavonoids extract from green tea was stronger than that of vitamin C.Conclusion:Under the control of the optimal technological conditions, the total flavonoids extracted from green tea with 80% acetylcholine-lactic acid solution as eutectic solvent had certain antioxidant activity.

Publication Date

7-4-2022

First Page

157

Last Page

162

DOI

10.13652/j.issn.1003-5788.2022.01.025

References

[1] 旷春桃,李湘洲,王玉霞,等.大叶冬青叶中总黄酮测定方法和提取工艺研究[J].食品科学,2009,30(6):49-51.KUANG Chun-tao,LI Xiang-zhou,WANG Yu-xia,et al.Study on determination methods and extraction process of total flavonoids in leaves of Ilex lati folia[J].Food Science,2009,30(6):49-51.
[2] 汪秋安,周冰,单扬.天然黄酮类化合物的抗氧化活性和提取技术研究进展[J].化工生产与技术,2004,11(5):29-35.WANG Qiu-an,ZHOU Bing,SHAN Yang.Progress on antioxidant activation and extracting technology of flavonoids[J].Chemical Production and Technology,2004,11(5):29-35.
[3] 陈金娥,杨雯婷,刘勇麟,等.微波萃取—响应面法优化绿茶黄酮提取工艺[J].食品研究与开发,2013,34(3):19-23.CHEN Jin-e,YANG Wen-ting,LIU Yong-lin,et al.Optimization of microwave-assisted extraction of flavonoids of green tea using response surface analysis[J].Food Research and Development,2013,34(3):19-23.
[4] 赵熙,粟本文,郑红发,等.响应面法优化超声波辅助提取绿茶总黄酮工艺的研究[J].茶叶科学,2010,30(4):295-301.ZHAO Xi,SU Ben-wen,ZHENG Hong-fa,et al.Study on optimization of ultrasound-assisted extraction technology of total flavonoids from green tea by response surface methodology[J].Tea Science,2010,30(4):295-301.
[5] 丘苑新,张泽雄,何娣,等.超声波辅助提取柚树叶黄酮及其抗氧化、抑菌能力研究[J].食品与机械,2021,37(6):174-178.QIU Yuan-xin,ZHANG Ze-xiong,HE Di,et al.Optimization of ultrasonic-assisted extraction technology of flavonoids from pomelo leaves and its antioxidant and antibacterial capabilities[J].Food & Machinery,2021,37(6):174-178.
[6] TAYLOR K M,TAYLOR Z E,HANDY S T.Rapid synthesis of aurones under mild conditions using a combination of microwaves and deep eutectic solvents[J].Tetrahedron Lett,2017,58(3):240-241.
[7] PAIVA A,CRAVEIRO R,AROSO I,et al.Natural deep eutectic solvents-solvents for the 21st century[J].ACS Sustainable Chemistry & Engineering,2014,2(5):1 063-1 071.
[8] ZHUANG Bo,DOU Li-li,LI Ping,et al.Deep eutectic solvents as green media for extraction of flavonoid glycosi DESs and aglycones from Platycladi Cacumen[J].Journal of Pharmaceutical and Biomedical Analysis,2017,134:214-219.
[9] BHUSHAN P C T,JADEJA G C.Microwave-assisted extraction for recovery of polyphenolic antioxidants from ripemango(Mangifer aindica L.)peel using lactic acid/sodium acetate deep eutectic mixtures[J].Food Science and Technology International,2020,26(1):78-92.
[10] RIBERIO B D,COELHO M A Z,MARRYCHO I M.Extraction of saponins from sisal(Agavesisalana)and jua(Ziziphusjoazeiro)with cholinium-based ionic liquid and deep eutectic solvents[J].European Food Research and Technology,2013,237:965-975.
[11] VAN OSCH D J G P,ZUBEIR L F,VAN DEN BRUINHORST A,et al.Hydrophobic deep eutectic solvents as water-immiscible extractants[J].Green Chemistry,2015,17(9):4 518-4 521.
[12] 谭传波,田华,赖琼玮,等.鲜榨山茶油中生物活性物质研究[J].粮食与油脂,2019,32(11):19-21.TAN Chuan-bo,TIAN Hua,LAI Qiong-wei,et al.Research on bioactive substances from fresh pressed camellia oil[J].Journal of Cereals & Oils,2019,32(11):19-21.
[13] LI Xi-can,LIN Jing,GAO Yao-xiang,et al.Antioxidant activity and mechanism of Rhizoma Cimicifugae[J].Chemistry Central Journal,2012,6(1):140.
[14] 张丽君,李敏,王静,等.石榴皮中黄酮类物质的提取及抗氧化研究[J].化学工程师,2021,35(9):65-68.ZHANG Li-jun,LI Min,WANG Jing,et al.Stud y on the extraction and antioxidant activity of flavones from pomegranate[J].Chemical Engineer,2021,35(9):65-68.
[15] 李杰,吴艳芳,王新胜,等.低共熔溶剂提取枳实中黄酮类成分的工艺研究[J].应用化工,2020,49(12):3 078-3 082.LI Jie,WU Yan-fang,WANG Xin-sheng,et al.Extraction of flavonoids from Fructus AurantiiImmaturus by deep eutectic solvent[J].Applied Chemical Industry,2020,49(12):3 078-3 082.
[16] 熊颖,钟海雁,周波.低共熔溶剂杏仁种皮提取物对油茶籽油氧化稳定性的影响[J].中国粮油学报,2021,36(5):102-107.XIONG Ying,ZHONG Hai-yan,ZHOU Bo.Effects of Apricot Kernel Coat Extracts with deep eutectic solvents on oxidation stability of camellia seed oil[J].Journal of the Chinese Cereals and Oils Association,2021,36(5):102-107.
[17] 阮怿航.富含天然活性成分的速溶茶加工工艺及其新产品研发[D].福州:福建农林大学,2020:7-38.RUAN Yi-hang.Research on processing technique of instant tea rich in nature active ingredients and its products[D].Fuzhou:Fujian Agriculture and Forestry University,2020:7-38.

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