Objective:This study aimed to improve the recrystallization yield and purity of flavonoid.Methods:Taking the crude flavonoids from pomelo peel extracted by ultrasonic-assisted ethanol extraction as raw materials, the process parameters of ethanol recrystallization and purification of flavonoids were optimized by response surface methodology.Results:The main factors affecting the recrystallization yield of flavonoids were ethanol concentration, crystallization time, pH value, and the interaction between ethanol concentration and pH value, among which, the ethanol concentration affacted most significantly.Conclusion: The optimal recrystallization conditions of flavonoids were as follows: ethanol volume fraction of 24%, liquid-solid ratio of 10∶1 (mL/g), pH 3.75, crystallization temperature of 4 ℃, and crystallization time of 24 h. The crystallization yield of flavonoids was 8.76%, and the main crystallization product was naringin with a purity of 83.38%.

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[1] 陈嘉景,彭昭欣,石梅艳,等.柑橘中类黄酮的组成与代谢研究进展[J].园艺学报,2016,43(2):384-400.CHEN J J,PENG Z X,SHI M Y,et al.Advances in on flavonoid composition and metabolism in citrus[J].Acta Horticulturae Sinica,2016,43(2):384-400.
[2] FRYDMAN A,WEISSHAUS O,BAR-PELED M,et al.Citrus fruit bitter flavors:Isolation and functional characterization of the gene Cm1,2RhaT encoding a 1,2 rhamnosyltransferase,a key enzyme in the biosynthesis of the bitter flavonoids of citrus[J].The Plant Journal,2004,40(1):88-100.
[3] LI P,WANG S,GUAN X,et al.Six months chronic toxicological evaluation of naringin in Sprague-Dawley rats[J].Food and Chemical Toxicology,2014,66:65-75.
[4] 郑培君,程海涛,王小博.柚子皮中的总黄酮提取工艺研究[J].湖北农业科学,2018,57(1):104-108.ZHENG P J,CHENG H T,WANG X B.Study on the processing condition of extracting total flavonoids from pomelo peel[J].Hubei Agricultral Science,2018,57(1):104-108.
[5] 孙慧慧,余元善,吴继军,等.沙田柚的加工和综合利用研究[J].食品研究与开发,2018,39(6):209-214.SUN H H,YU Y S,WU J J,et al.Study on production and comprehensive utilization of citrus grandis osbeck[J].Food Research and Development,2018,39(6):209-214.
[6] 于宏伟,谷维娜,李娜,等.柚皮苷的提取方法及其应用研究进展[J].湖北农业科学,2011,50(8):1 516-1 518.YU H W,WU W N,LI N,et al.Research progress of naringin[J].Hubei Agricultural Sciences,2011,50(8):1 516-1 518.
[7] 丘苑新,张泽雄,何娣,等.超声波辅助提取柚树叶黄酮及其抗氧化、抑菌能力研究[J].食品与机械,2021,37(6):174-178.QIU Y X,ZHANG Z X,HE D,et al.Optimization of ultrasonic-assisted extraction technology of flavonoids from pomelo leaves and its antioxidant and antibacterial capabilities[J].Food & Machinary,2021,37(6):174-178.
[8] 高丽,张昭,李静.大孔树脂精制荷叶黄酮的工艺研究[J].粮食与油脂,2021,34(3):67-69,89.GAO L,ZHANG Z,LI J.Research on the technology of refining flavonoidfrom lotus leaf by macroporous adsorption resin[J].Cereals and Oils,2021,34(3):67-69,89.
[9] 康彤.甘草黄酮的提取、纯化及活性研究[D].天津:天津科技大学,2019:49-50.KANG T.Exreation,purification and activity of flavonoids from glycyrrhiza[D].Tianjing:Tianjing University of Science and Technology,2019:49-50.
[10] 江正祥,李国防.优先结晶法纯化曲克芦丁[J].应用化工,2010,39(11):1 632-1 634.JIANG Z X,LI G F.Purification of troxerutin by preferential crystallization[J].Applied Chemical Industry,2010,39(11):1 632-1 634.
[11] 刘艳杰.苦荞总黄酮的纯化与芦丁水溶性粉体的研究[D].哈尔滨:东北林业大学,2018:18-27.LIU Y J.Study on purfication of flanovoids from tartaty buckwheat and water souble power of rutin[D].Harbin:Northeast Forestry University,2018:18-27.
[12] 项飞.胡柚皮膳食黄酮的提取[D].武汉:武汉工程大学,2016:31-34.XIANG F.Extractionofdietaryflavonoids from Huyou peels[D].Wuhan:Wuhan Institute of Technology,2016:31-34.
[13] 苏艳兰,刘功德,艾静汶,等.原味柚子皮加工质量关键控制点的确定与控制[J].大众科技,2016,18(10):30-31.SU Y L,LIU G D,AI J W,et al.Determination and control of key quality control points for the processing of natural-taste pomelo peel[J].Popular Science and Technology,2016,18(10):30-31.
[14] 李歌,王珊珊,丁文珺,等.柚皮中黄酮类化合物提取工艺研究[J].生物技术进展,2016,6(2):125-129.LI G,WANG S S,DING W J,et al.Research on extration process of flavonoids from pomelo peel[J].Current Biotechnology,2016,6(2):125-129.
[15] 晏幸,戴宇琪,欧丽明,等.溪蜜柚柚皮黄酮提取工艺优化[J].食品工业科技,2020,41(17):135-140.YAN X,DAI Y Q,OU L M,et al.Optimization of flavonoids extraction from Guanxi honey pomelo peel[J].Science and Technology of Food Industry,2020,41(17):135-140.
[16] 林芳湄.微波辅助提取柚皮苷的研究分析[J].广州化工,2021,49(22):55-57.LIN F M.Study on microwave-assisted extraction of naringin[J].Guangzhou Chemical Industry,2021,49(22):55-57.
[17] 甄畅迪,王蕴波,张毅,等.化橘红有效成分柚皮苷的提取分离[J].佛山科学技术学院学报(自然科学版),2010,28(3):55-58.ZHEN C D,WANG Y B,ZHANG Y,et al.Extraction and separation of naringin from exocarpium citri grandis[J].Journal of Foshan University(Natural Science Edition),2010,28(3):55-58.
[18] 阳梅芳.柚子黄酮类物质提取、分离及生物特性研究[D].广州:华南理工大学,2013:18-22.YANG M F.Study on the extraction,separation and biological characteristics of the flavonoids in grapefruit[D].Guangzhou:South China Univerisity of Technology,2013:18-22.
[19] 许鹭.柚皮中柚皮苷的提取、分离纯化及其固体分散体与卵磷脂复合物的制备及表征[D].广州:华南理工大学,2015:25-30.XU L.Extraction and purification of naringin in grapefruit peel and preparation and characterization of its solid dispersion and lecithin compound[D].Guangzhou:South China Univerisity of Technology,2015:25-30.
[20] 涂招秀,谢欣,胡居吾,等.蔓三七叶黄酮提取工艺优化及其体外抗氧化活性研究[J].生物化工,2018,4(6):1-6.TU Z X,XIE X,HU J W,et al.Optimization of flavonoids extraction process of flavonoids from gynura provumbens leaves and evaluation of antioxidative activity[J].Biological Chemical Engineering,2018,4(6):1-6.
[21] 贾冬英.柚皮苷提取纯化及其络合改性研究[D].成都:四川大学,2002:27-33.JIA D Y.Exraction,purification and chelation of narigin[D].Chengdu:Sichuan University,2002:27-33.

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