Objective:The refining parameters of isoflavones from Puerariae flos were optimized by using HPD-100 macroporous adsorption resin, and its in vitro lipid-lowering effects were evaluated. Methods:The optimized refine parameters of isoflavones from Puerariae flos were studied by macroporous adsorption resin, and the lipid-lowering effects of isoflavones from Puerariae flos were evaluated by pancreatic lipase enzyme activity, pancreatic cholesterol esterase enzyme activity and micellar cholesterol solubility. Results:Adsorption parameters: Puerariae flos isoflavones were adsorbed with concentration of 100 mg/mL, pH 6.0 and upper column volume of 20 mL. Desorption parameters: the adsorption resins were washed by 70% ethanol with pH 5.0 at flow rate of 2.0 mL/min. The purity of refined Puerariae flos isoflavones were 81.47%. The inhibition ratio on pancreatic lipase enzyme, pancreatic cholesterol esterase enzyme and micellar cholesterol solubility were 40.73%, 52.32% and 46.17%, respectively, and these were increased by 26.65%, 47.46% and 37.53% respectively, compared with not refining. Conclusion:Puerariae flos isoflavones showed lipid-lowering effects by inhibiting pancreatic lipase enzyme activity, pancreatic cholesterol esterase enzyme activity and micellar cholesterol solubility.

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[1] 王兰,蓝璟,龚频,等.葛根异黄酮降血糖活性及作用机制的研究[J].食品科技,2017,42(3):223-226.WANG Lan,LAN Jing,GONG Pin,et al.Hypoglycemic effects and mechanisms of Radix Puerariae isoflavone[J].Food Science and Technology,2017,42(3):223-226.
[2] 王金凤,薄华本,孟祥颖,等.葛花异黄酮对急性心肌梗死小鼠心肌的保护作用[J].中国药理学通报,2010,26(1):59-62.WANG Jin-feng,PU Hua-ben,MENG Xiang-ying,et al.The protection of isoflavones on myocardium in myocardial infarction mouse[J].Chinese Pharmacological Bulletin,2010,26(1):59-62.
[3] YAO H,WU B,CHENG Y,et al.High throughput chemiluminescence platform for evaluating antioxidative activity of total flavonoid glycosides from plant extracts[J].Food Chemistry,2009,115(1):380-386.
[4] KAMIYA T,SAMESHIMA-KAMIYA M,NAGAMINE R,et al.The crude extract from Puerariae flower exerts antiobesity and antifatty liver effects in high-fat diet-induced obese mice[J].Evidence-Based Complementary and Alternative Medicine,2012,2 012(8):1-6.
[5] 戴雨霖,于珊珊,张颖,等.葛花中异黄酮类化学成分的RRLC-Q-TOF MS/MS研究[J].高等学校化学学报,2014,35(7):1 396-1 402.DAI Yu-lin,YU Shan-shan,ZHANG Ying,et al.RRLC-Q-TOF MS/MS study on isoflavones from Pueraria puerariae[J].Chemical Journal of Chinese Universities,2014,35(7):1 396-1 402.
[6] 吴桐,黄彧,侯万超,等.葛花中异黄酮类活性成分的分离及鉴定[J].分析化学学报,2020,36(3):353-358.WU Tong,HUANG Yu,HOU Wan-chao,et al.Isolation and identification of isoflavones from Pueraria[J].Journal of Analytical Sciences,2020,36(3):353-358.
[7] WU T,LIU C,HUANG Y,et al.Simultaneous screening and isolation of activated constituents from Puerariae Flos by ultrafiltration with liquid chromatography and mass spectrometry combined with high-speed counter-current chromatography[J].Journal of Separation Science,2018,41(24):4 458-4 468.
[8] MAROUNEK M,VOLEK Z,SKRIVANOVÁE,et al.Comparative study of the hypocholesterolemic and hypolipidemic activity of alginate and amidated alginate in rats[J].International Journal of Biological Macromolecules,2017,105:620-624.
[9] SIRICHAIWETCHAKOON K,LOWE G M,THUMANU K,et al.The effect of Pluchea indica(L.)less.tea on adipogenesis in 3T3-L1 adipocytes and lipase activity[J].Evidence-based Complementary and Alternative Medicine,2018,2 018(2):1-13.
[10] ADISAKWATTANA S,INTRAWANGSO J,HEMRID A,et al.Extracts of edible plants inhibit pancreatic lipase,cholesterol esterase and cholesterol micellization,and bind bile acids[J].Food Technology & Biotechnology,2012,50(1):11-16.
[11] SIVASHANMUGAM T,MUTHUKRISHNAN S,UMAMAHESWARI M,et al.Cholesterol esterase inhibitory activity of flavonoids using in silico and in vitro studies[J].Asian Pacific Journal of Tropical Disease,2013,3:930-935.
[12] 何桂香,刘金宝.鹰嘴豆异黄酮提取物对高脂血症小鼠的降脂作用[J].中国临床康复,2005,9(7):80.HE Gui-xiang,LIU Jin-bao.Effect of extract of Cicer arietium L.isoflavones in decreasing blood lipid of mice with hyperlipidemia[J].Chinese Journal of Clinical Rehabilitation,2005,9(7):80.
[13] 孙明哲.超声波辅助提取葛花总异黄酮及体外抗氧化活性研究[J].食品研究与开发,2020,41(3):29-34.SUN Ming-zhe.Ultrasound-assisted extraction of total isoflavones from Puerariae flos and its antioxidant activity[J].Food Research and Development,2020,41(3):29-34.
[14] GONDOIN A,GRUSSU D,STEWART D,et al.White and green tea polyphenols inhibit pancreatic lipase in vitro[J].Food Research International,2010,43(5):1 537-1 544.
[15] GARZÓN A G,CIAN R E,AQUINO M E,et al.Isolation and identification of cholesterol esterase and pancreatic lipase inhibitory peptides from brewerʼs spent grain by consecutive chromatography and mass spectrometry[J].Food & Function,2020,11(6):4 994-5 003.
[16] PRADOS I M,LUISA MARINA M,CONCEPCIÓN G M.Isolation and identification by high resolution liquid chromatography tandem mass spectrometry of novel peptides with multifunctional lipid-lowering capacity[J].Food Research International,2018,111(9):77-86.
[17] 张星,董欣,刘振春,等.大孔树脂纯化黑豆异黄酮的工艺优化[J].西北农林科技大学学报(自然科学版),2018,46(1):136-145,154.ZHANG Xing,DONG Xin,LIU Zhen-chun,et al.Optimization of purification process of black beans isoflavones with macroporous adsorption resin[J].Journal of Northwest A & F University(Natural Science Edition),2018,46(1):136-145,154.
[18] 闵玉涛,宋彦显,陶敬,等.AB-8型大孔树脂分离纯化葛根异黄酮的工艺优化[J].食品工业,2015,36(7):159-162.MIN Yu-tao,SONG Yan-xian,TAO Jing,et al.Optimized the extraction and partial purification of isoflavone in pueraria lobata tubers by Ab-8 macroporous resin[J].The Food Industry,2015,36(7):159-162.
[19] 刘莹,郁建平.大孔树脂纯化葛花总异黄酮的工艺条件研究[J].山地农业生物学报,2010,29(1):56-60.LIU Ying,YU Jian-ping.Separation and purification of the isoflavanoids from flos puerariae using macroporous resin[J].Journal of Mountain Agriculture and Biology,2010,29(1):56-60.
[20] 霍梦恩,李冬利,罗小燕,等.陈年乌龙茶的生化成分及其降脂降糖活性研究[J].茶叶通讯,2019,46(4):472-478.HUO Meng-en,LI Dong-li,LUO Xiao-yan,et al.Study on the biochemical composition of aged oolong teas and its hpyerglycemic and hypolipidemic activities in vitro[J].Journal of Tea Communication,2019,46(4):472-478.
[21] LI B,ZHOU B,LU H,et al.Phosphaisocoumarins as a new class of potent inhibitors for pancreatic cholesterol esterase[J].European Journal of Medicinal Chemistry,2010,45(5):1 955-1 963.
[22] 苏建辉,马朝阳,杨鹿,等.槲皮素、EGCG对胆固醇酯酶活性和胆固醇胶束抑制作用研究[J].食品工业科技,2015,36(11):346-349.SU Jian-hui,MA Chao-yang,YANG Lu,et al.Inhibition of pancreatic cholesterol esterase activities and cholesterol micelle of EGCG and quercetin[J].Science and Technology of Food Industry,2015,36(11):346-349.
[23] 包小兰,刘晓静,郑睿,等.亚麻籽降胆固醇活性肽的酶解工艺优化及分级制备[J].中国油脂,2020,45(6):37-42.BAO Xiao-lan,LIU Xiao-jing,ZHENG Rui,et al.Optimization of enzymatic hydrolysis and fractionation preparation of flaxseed peptides with cholesterol-lowering activity[J].China Oils and Fats,2020,45(6):37-42.
[24] ZHANG J,ZHANG W,MAMADOUBA B,et al.A comparative study on hypolipidemic activities of high and low molecular weight chitosan in rats[J].International Journal of Biological Macromolecules,2012,51(4):504-508.



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