Abstract
Objective:The research is to explore the enzyme inhibition of Perilla crude extract, and to preliminarily elucidate the pharmacological substance basis of them on hyperglycemia, hyperuric acid and hyperlipidemia.Methods:The content of flavonoids and phenolic acid in UV extract was determined and showed the active components associated with enzyme inhibition. In vitro experiments, the inhibition of Perilla extracts on different enzyme activity was determined and the inhibitory activity was evaluated by IC50. Molecular docking was used to predict the targets of Perilla crude extract on pancreatic lipase and amylase inhibition.Results:Flavone content in the crude UV extract was 0.309 8 mg/mg, and the total phenolic acid content was 58.59 mg/g. The IC50 values of Perilla crude extractd for α-amylase, xanthine oxidase, pancreatic lipase and acetylcholinesterase were 0.273, 0.244, 0.347 and 0.018 mg/mL, respectively.Apigenin-5-O-β-D-glucosine, xyrhinin-7-diglucosotide, apigenin-7-glucosine, cannol-3-O-ruassin, rosemary acid, rutin and others were the effective active components of perilla crude extracts.Conclusion:The Perilla crude extracts all have certain inhibitory activities on four enzymes, and presents a dose-effect relationship.
Publication Date
7-20-2022
First Page
183
Last Page
188
DOI
10.13652/j.spjx.1003.5788.2022.90044
Recommended Citation
Yu-ting, YU; Yanl, ZHANG; Ying, ZHANG; Na-na, LI; Yan, CHENG; Meng-di, ZHENG; Ping, YAN; and Xi-yan, CHAI
(2022)
"Explore the inhibitory effect of Perilla crude extract on metabolic syndrome-related enzymes based on molecular docking,"
Food and Machinery: Vol. 38:
Iss.
4, Article 30.
DOI: 10.13652/j.spjx.1003.5788.2022.90044
Available at:
https://www.ifoodmm.cn/journal/vol38/iss4/30
References
[1] 张运晖,赵瑛,欧巧明.紫苏叶化学成分及生物活性研究进展[J].甘肃农业科技,2020(12):69-76.ZHANG Yun-hui,ZHAO Ying,OU Qiao-ming.Progress in the chemical composition and bioactivity of Perilla leaves[J].Gansu Agricultural Science and Technology,2020(12):69-76.
[2] 王亚凤,张祖湘,何瑞杰,等.黄精地上部位化学成分及其胰脂肪酶抑制活性研究[J].天然产物研究与开发,2020,32(11):1 811-1 817.WANG Ya-feng,ZHANG Zu-xiang,HE Rui-jie,et al.Study on the aboveground chemical composition of yellow essence and the inhibitory activity of pancreatic lipase[J].Research and Development of Natural Products,2020,32(11):1 811-1 817.
[3] 潘颖,孔令东.中药干预代谢综合征的基础研究进展[J].世界科学技术:中医药现代化,2016,18(9):1 441-1 446.PAN Ying,KONG Ling-dong.Basic research progress of traditional Chinese medicine intervention in metabolic syndrome[J].World Science and Technology:Modernization of Traditional Chinese Medicine,2016,18(9):1 441-1 446.
[4] 李可,吴成亚,张秀媛.五苓散加减治疗痰浊内阻证老年肥胖代谢综合征临床疗效及对炎症指标的影响[J].湖北中医药大学学报,2021,23(3):103-106.LI Ke,WU Cheng-ya,ZHANG Xiu-yu.The clinical effect of senile obesity metabolic syndrome and its effect on the inflammatory index[J].Journal of Hubei University of Traditional Chinese Medicine,2021,23(3):103-106.
[5] 刘莉,李卫忠,王师菡,等.中医药多靶点干预代谢综合征机制研究概述[J].中国实验方剂学杂志,2021,27(3):214-221.LIU Li,LI Wei-zhong,WANG Si-han,et al.Overview of the mechanism of TCM multi-target intervention in metabolic syndrome[J].Chinese Journal of Experimental Square Pharmacology,2021,27(3):214-221.
[6] WU Hao-ran,TIAN Jia-xing,DAI Dan,et al.Efficacy and safety assessment of traditional Chinese medicine for metabolic syndrome[J].BMJ Open Diabetes Research & Care,2020,8(1):e001181.
[7] 杨海洋,汪国鹏,杨文宁,等.黄芩素、槲皮素、高良姜素对黄嘌呤氧化酶靶点占有率的模拟分析[J].中国实验方剂学杂志,2021,27(14):147-154.YANG Hai-yang,WANG Guo-peng,YANG Wen-ning,et al.Simulation analysis of xuletin and xerine oxidase target[J].Chinese Journal of Experimental Square Pharmacology,2021,27(14):147-154.
[8] 洪鑫月,吴健妹,罗小乔,等.多酚化合物对黄嘌呤氧化酶抑制作用的研究进展[J].食品与机械,2021,37(2):1-8.HONG Xin-yue,WU Jian-mei,LUO Xiao-qiao,et al.Research progress of inhibitory effects of polyphenolic compounds on xanthine oxidase[J].Food & Machinery,2021,37(2):1-8.
[9] 陈雨涔,王莹莹,杜若童,等.槲皮素、芦丁、没食子酸抑制黄嘌呤氧化酶的活性及动力学特性[J].现代食品科技,2020,36(12):118-124.CHEN Yu-cen,WANG Ying-ying,DU Ruo-tong,et al.Inhibitory effect of quercetin,rutin and gallic acid on xanthine oxidase and kinetic characteristics[J].Modern Food Science and Technology,2020,36(12):118-124.
[10] 〖JP2〗赵二劳,赵三虎,范建凤,等.天然产物中活性成分对α-淀粉酶抑制作用研究进展[J].食品研究与开发,2020,41(19):199-203.ZHAO Er-luo,ZHAO San-hu,FAN Jian-feng,et al.Progress in the inhibition of active ingredients in natural products on α-amylase[J].Food Research and Development,2020,41(19):199-203.
[11] 张剑,张博,贺茂芳,等.毛细管电泳法测定天麻素对乙酰胆碱酯酶活性的抑制[J].色谱,2020,38(9):1 102-1 106.ZHANG Jian,ZHANG Bo,HE Mao-fang,et al.Determination of gastrodin activity inhibition on acetyIcholinesterase by capillary electrophoresis[J].Chromatography,2020,38(9):1 102-1 106.
[12] 范三红,贾槐旺,张锦华,等.不同提取方法对紫苏籽粕蛋白功能性质的影响[J].中国调味品,2021,46(12):61-69.FAN San-hong,JIA Huai-wang,ZHANG Jin-hua,et al.Effects of different extraction methods on the functional properties of perilla seed meal protein[J].Chinese Condiments,2021,46(12):61-69.
[13] 郭旭,田荣荣,张东.紫苏油的提取工艺和药理功能研究进展[J].粮油食品科技,2021,29(5):120-130.GUO Xu,TIAN Rong-rong,ZHANG Dong.Research progress on extraction technology and pharmacological functions of perilla oil[J].Grain,Oil and Food Technology,2021,29(5):120-130.
[14] 谭永兰.紫苏籽壳多酚的提取及应用研究[D].太原:中北大学,2019:8-9.TAN Yong-lan.Extraction and application of Perilla seed shell polyphenol[D].Taiyuan:North Central University,2019:8-9.
[15] 李晓雪.紫苏黄酮的提取工艺优化、含量分析及降糖活性研究[D].哈尔滨:东北林业大学,2020:9-10.LI Xiao-xue.Optimization of extraction technology,content analysis and hypoglycemic activity of flavonoids from Perilla[D].Harbin:Northeast Forestry University,2020:9-10.
[16] 曾傲琼,让一峰,杨瑞金,等.条斑紫菜多糖对α-淀粉酶的抑制效果[J].中国生物制品学杂志,2019,32(4):396-402.ZENG Ao-qiong,RANG Yi-feng,YANG Rui-jin,et al.Inhibition effect of striped seaweed polysaccharide on α-amylase[J].Chinese Journal of Biology Products,2019,32(4):396-402.
[17] 田丝竹,李绪文,臧爽,等.10种野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用及其酚类化合物含量和抗氧化活性研究[J].分析化学,2021,49(3):449-459.TIAN Si-zhu,LI Xu-wen,ZANG Shuang,et al.Ininhibitory effect of ten wild plant fruits on α-amyase and tyrossinase and their phenolic compound content and antioxidant activity[J].Analytical Chemistry,2021,49(3):449-459.
[18] 张瑛毓,普布多吉,卢聪,等.基于黄嘌呤氧化酶活性抑制和斑马鱼高尿酸血症模型的降尿酸功效食药材筛选[J].食品工业科技,2021,42(12):334-339.ZHANG Ying-yu,PU Bu-do-ji,LU Cong,et al.Screening of uric acid lowering materials based on xanthine oxidase inhibition and zebrafish hyperuricemia model[J].Food Industry Science and Technology,2021,42(12):334-339.
[19] 王远,郑雯,蔡珺珺,等.辣木叶黄酮结构分析及其对胰脂肪酶的抑制作用[J].食品科学,2018,39(2):31-37.WANG Yuan,ZHENG Wen,CAI Jun-jun,et al.Structural analysis of Moringa leaves and its inhibitory effect on pancreatic lipase[J].Food Science,2018,39(2):31-37.