Objective:To understand bacterial composition and obtain isolated fermentative strains found in kombucha.Methods:The bacterial composition and dominant strains were analyzed using 16S rDNA high-throughput sequencing technology and traditional microbial culture method.Results:High-throughput sequencing showed that the bacterial community is richer and more diverse than the fungi community. Komagataeibacter,Acetobacter and Lactobacillus were the dominant bacterial strains, while Dekkera and Zygosacchaormyces were the dominant fungal strains. At the same time, 7 strains of acetic acid bacteria, 3 strains of yeast and 1 strain of lactic acid bacteria were isolated by traditional plate culture method, including 1 strain of Komagataeibacter saccharivorans, 1 strain of Acetobacter malorum, 4 strains of Komagataeibacter xylinus, 1 strain of Acetobacter, 2 strains of Zygosaccharomyces bailii, 1 strain of Dekkera bruxelensis and 1 strain of Lactobacillus plantarum.Conclusion:The results from high throughput sequencing were consistent with those obtained from traditional culture method.

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[1] MINDANI I,NILAKSHI J,CANDY C,et al.Retracted:Application of the Kombucha ‘tea fungus’ for the enhancement of antioxidant and starch hydrolase inhibitory properties of ten herbal teas[J].Food Chemistry,2016,194(1):304-311.
[2] XIA X D,DAI Y Q,WU H,et al.Kombucha fermentation enhances the health-promoting properties of soymilk beverage[J].Journal of Functional Foods,2019,62:103549.
[3] SOMNATH C,SEMANTEE B,ANTONIS C,et al.Kombucha tea fermentation:Microbial and biochemical dynamics[J].International Journal of Food Microbiology,2016,220(30):63-72.
[4] 徐伟,葛阳阳,陈翠婷,等.基于宏基因组技术分析传统红茶菌中菌群组成及其主要代谢通路[J].食品工业科技,2018,39(5):119-129.XU Wei,GE Yang-yang,CHEN Cui-ting,et al.Microorganism composition and main metabolic pathways analysis of traditionalKombucha by metagenomic technology[J].Technology Science and Technology of Food Industry,2018,39(5):119-129.
[5] GAO F F,ZENG G H,WANG B,et al.Discrimination of the geographic origins and varieties of wine grapes using high-throughput sequencing assisted by a random forest model[J].LWT-Food Science and Technology,2021,145:111333.
[6] 李洋,赵欣,张玉,等.牦牛酸乳中耐酸耐胆盐乳酸菌的分离筛选和鉴定[J].食品与机械,2018,34(7):23-28.LI Y,ZHAO X,ZHANG Y,et al.Separation and screening of lactic acid bacteria from the traditional yak yogurt resistant against of acid and bile salts[J].Food & Machinery,2018,34(7):23-28.
[7] LIN S P,HUANG Y H,CHEN Y K,et al.Isolation and identification of cellulose-producing strain Komagataeibacter intermedius from fermented fruit juice[J].Carbohydrate Polymers,2016,151(20):827-833.
[8] RUAN R,FENG T,LI Y,et al.Screening and identification of female-specific DNA sequences in octaploid sturgeon using comparative genomics with high-throughput sequencing[J].Genomics,2021,113(6):4 237-4 244.
[9] GULITZ A,STADIE J,EHRMANN M,et al.Comparative phylobiomic analysis of the bacterial community of water kefir by 16S rRNA gene amplicon sequencing and ARDRA analysis[J].Appl Microbiol,2013,114(4):1 082-1 091.
[10] 王洁琛,陈志周,王颖,等.红茶菌中醋酸菌和酵母菌的分离鉴定及其相互作用[J].中国酿造,2020,39(3):126-130.WANG Jie-chen,CHEN Zhi-zhou,WANG Ying,et al.Isolation,identification and interaction of acetic acid bacteria and yeast in kombucha[J].Chinese Brew,2020,39(3):126-130.
[11] 郭志华,张兴桃,段腾飞,等.泡菜中降解亚硝酸盐乳酸菌的筛选及生物学特性研究[J].食品与发酵工业,2019,45(17):66-72.GUO Zhi-hua,ZHANG Xing-tao,DUAN Teng-fei,et al.Screening and biological characterization of nitrite degrading lactic acid bacteria in kimchi[J].Food and Fermentation Industries,2019,45(17):66-72.
[12] 陈宠,苏亚平,岳雅欣,等.海棠酵素微生物菌相分析及其功能初步研究[J].中国酿造,2020,39(8):70-76.CHEN Chong,SUN Ya-ping,YUE Ya-xin,et al.Analysis of microbial community in Malus pruniflolia Jiaosu and preliminary study on its function[J].Chinese Brew,2020,39(8):70-76.
[13] JIANG Y,LU X,ZHANG C,et al.Microbial dynamics and flavor formation during the traditional brewing of Monascus vinegar[J].Food Research International,2019,125:108531.
[14] PEI J J,JIN W G,GUO X Y,et al.Isolation,purification,and structural identification of a new bacteriocin made by Lactobacillus plantarum found in conventional kombucha[J].Food Control,2020,110:106923.
[15] 王春龙.红茶菌发酵饮料中的菌群分析与功能评价[D].天津:天津科技大学,2017:20-27.WANG Chun-long.Microflora analysis and functional evaluation of kombucha fermented beverage[D].Tianjin:Tianjin University of Science and Technology,2017:20-27.
[16] HIRST M B,RICHTER C L.Review of aroma formation through metabolic pathways of Saccharomyces cerevisiae in beverage fermentations[J].American Journal of Enology and Viticulture,2016,67(4):361-370.
[17] DE ROOS J,DE VUYST L.Acetic acid bacteria in fermented foods and beverages[J].Current Opinion in Biotechnology,2018,49:115-119.
[18] AHMET E,YETIMAN ZULAL K.Identification of acetic acid bacteria in traditionally produced vinegar and mother of vinegar by using different molecular techniques[J].International Journal of Food Microbiology,2015,204:9-16.
[19] GAGGÍA F,BAFFONI L,GALIANO M,et al.Kombucha beverage from green,black and rooibos teas:A comparative study looking at microbiology,chemistry and antioxidant activity[J].Nutrients,2019,11(1):1-22.
[20] MARSH A J,O’SULLIVAN O,HILL C,et al.Sequence-based analysis of the bacterial and fungal compositions of multiple kombucha(tea fungus)samples[J].Food Microbiology,2014,38(1):171-178.
[21] 赵沁雨,兰天,袁取予,等.植物乳杆菌发酵对果汁品质的影响研究概述[J].食品与发酵工业,2021,47(16):300-307.ZHAO Qin-yu,LAN Tian,YUAN Qu-yu,et al.Research progress on the effect of Lactobacillus plantarum fermentation on juice quality[J].Food and Fermentation Industries,2021,47(16):300-307.
[22] LIN R Q,SUN Y,MU P Q,et al.Lactobacillus rhamnosus GG supplementation modulates the gut microbiota to promote butyrate production,protecting against deoxynivalenol exposure in nude mice[J].Biochemical Pharmacology,2020,175:113868.

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