Objective: The aim of the study was to decrease the transportation cost while reducing the nutrition loss of Urechis unicinctus. Methods: Chitosan was used for pretreatment of Urechis unicinctus, and the changes of the main nutritional and physiological indexes of the three groups i.e., control group, after waterless preservation group and recovered group, were measured. Results: The optimal concentration of chitosan was 3 g/L. After being pretreated by chitosan, the survival rate of Urechis unicinctus could reach 67% after 64 h of waterless preservation. The survival rate, muscle glycogen, water holding capacity of the experimental group were all significantly higher than those of the control group (P<0.05). Compared with those groups without recovered, the content of crude protein, lactic acid, crude fat, and water were all dramatically decreased after recovered no matter in control group or in experimental group (P<0.01). Conclusion: Chitosan can improve the survival rate and quality of Urechis unicinctus after waterless preservation. This research provides reference for the study on waterless preservation method of Urechis unicinctus, and has certain application research value.

Publication Date


First Page


Last Page





[1] 程泽宇,赵佳锐,肖翰,等.单环刺螠的活性成分及其在调味品开发中的应用研究进展[J].食品科技,2020,45(4):253-258.CHENG Ze-yu,ZHAO Jia-rui,XIAO Han,et al.Study on the bioactive components of Urechis unicinctus and the development of condiments[J].Food Science and Technology,2020,45(4):253-258.
[2] 王力勇,胡丽萍,赵强,等.单环刺螠亲体运输及暂养技术研究[J].中国水产,2017(4):93-94.WANG Li-yong,HU Li-ping,ZHAO Qiang,et al.Research on Urechis unicinctus parental transport and hiatus technologies[J].China Fisheries,2017(4):93-94.
[3] KING H R.Fish transport in the aquaculture sector:An overview of the road transport of Atlantic salmon in Tasmania[J].Journal of Veterinary Behavior:Clinical Applications and Research,2008,4(4):163-168.
[4] ZHANG Yong-jun,ZHANG Xiao-shuan,NGA M T T,et al.Development and evaluation of key ambient factors online monitoring system in live Urechis unicinctus transportation strategies[J].Computers and Electronics in Agriculture,2018,145:43-52.
[5] WANG Wen-sheng,ZHANG Yong-jun,LIU Yan,et al.Effects of waterless live transportation on survivability,physiological responses and flesh quality in Chinese farmed sturgeon(Acipenser schrenckii)[J].Aquaculture,2020,518(C):734834.
[6] HE Rui-peng,SU Yue-peng,WANG An-li,et al.Survival and serum biochemical responses of spotted sea bass Lateolabrax maculatus during simulated waterless live transportation[J].Aquaculture Research,2020,51(9):3 495-3 505.
[7] 何蓉,谢晶.水产品保活技术研究现状和进展[J].食品与机械,2012,28(5):243-246.HE Rong,XIE Jing.Current status and advances in studies on technology of keeping alive of aquatic products[J].Food & Machinery,2012,28(5):243-246.
[8] 刘嘉莉,蓝蔚青,刘大勇,等.壳聚糖在水产品保鲜中应用研究进展[J].食品与机械,2019,35(3):231-236.LIU Jia-li,LAN Wei-qing,LIU Da-yong,et al.Research progress of chitosan in aquatic products preservation[J].Food & Machinery,2019,35(3):231-236.
[9] MA Zheng-xin,GARRIDO-MAESTU A,JEONG K C.Application,mode of action,and in vivo activity of chitosan and its micro and nanoparticles as antimicrobial agents:A review[J].Carbohydrate Polymers:Scientific and Technological Aspects of Industrially Important Polysaccharides,2017,176:257-265.
[10] LI Ting-ting,LI Jian-rong,HU Wen-zhong,et al.Quality enhancement in refrigerated red drum(Sciaenops ocellatus)fillets using chitosan coatings containing natural preservatives[J].Food Chemistry,2013,138(2/3):821-826.
[11] ALISHAHI A,AIDER M.Applications of chitosan in the seafood industry and aquaculture:A review[J].Food and Bioprocess Technology,2012,5(3):817-830.
[12] 高磊,赫崇波,鲍相渤,等.工厂化养殖仿刺参营养品质分析与评价[J].水产学报,2016,40(2):243-254.GAO Lei,HE Chong-bo,BAO Xiang-bo,et al.Analysis and appraisement of nutrient quality for sea cucumber Apostichopus japonicus in industrial culture[J].Journal of Fisheries of China,2016,40(2):243-254.
[13] 叶丽红,许艳顺,夏文水,等.Κ-卡拉胶、复合磷酸盐和蛋清粉对高水分鱼丸水分和质构特性的影响[J].食品科技,2019,44(4):291-297.YE Li-hong,XU Yan-shun,XIA Wen-shui,et al.The effect of Κ-carrageenan,composite phosphate and egg white powder on the moisture and texture characteristics of high moisture fish balls[J].Food Science and Technology,2019,44(4):291-297.
[14] 陈燕园.缢蛏糖原代谢关键基因多态性与糖原含量、生长性状的相关性分析[D].上海:上海海洋大学,2020:9.CHEN Yan-yuan.Correlation analysis of polymorphism of glycogen metabolism key genes and glycogen content and growth traits of Sinonovacula constricta[D].Shanghai:Shanghai Ocean University,2020:9.
[15] 曹杰,王琪,梅俊,等.有水与无水保活运输对大菱鲆生理应激及鱼肉品质的影响[J].水产学报,2021,45(7):1 034-1 042.CAO Jie,WANG Qi,MEI Jun,et al.Effects of transport in water and waterless transport on physiological stress and flesh quality of turbot(Scophthalmus maximus)[J].Journal of Fisheries of China,2021,45(7):1 034-1 042.
[16] 夏玉秀,申旭红,任爽宁,等.不同干露条件单环刺螠的耐受性及对体表黏液特性的影响[J].大连海洋大学学报,2019,34(4):538-544.XIA Yu-xiu,SHEN Xu-hong,REN Shuang-ning,et al.Air exposure tolerance and characteristics of surface mucus in echiuran worm Urechis unicinctus in wet sand with and without water[J].Journal of Dalian Ocean University,2019,34(4):538-544.
[17] 张新明,李慷均.单环刺螠生物学及生理学研究进展[J].河南农业科学,2011,40(11):26-29.ZHANG Xin-ming,LI Kang-jun.Research progress on biology and physiology of Urechis uniconctus[J].Journal of Henan Agricultural Sciences,2011,40(11):26-29.
[18] 吕慧超.单环刺螠体表黏液的制备与自溶现象的研究[D].烟台:烟台大学,2021:44-45.LU Hui-chao.Preparation of surface mucous and study on autolysis of Urechis unicinctus[D].Yantai:Yantai University,2021:44-45.
[19] 裴诺,施文正,汪之和.壳聚糖与生物保鲜剂复合使用在水产品保鲜中的研究进展[J/OL].食品工业科技.[2021-10-18].https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CAPJ&dbname=CAPJLAST&filename=SPKJ2021070500A&uniplatform=NZKPT&v=RUHrlx_8YDAkSnWt8OZi3GYIMeURAucPt6Q3q0 gY3_EM-9kyUi23uTmqSkPZupwK.PEI Nuo,SHI Wen-zheng,WANG Zhi-he.Research progress in the application of chitosan and biological preservative in aquatic products preservation[J/OL].Science and Technology of Food Industry.[2021-10-18].https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CAPJ&dbname=CAPJLAST&filename=SPKJ202107 0500A&uniplatform=NZKPT&v=RUHrlx_8YDAkSnWt8OZi3 GYIMeURAucPt6Q3q0gY3_EM-9kyUi23uTmqSkPZupwK.
[20] 范秀萍,秦小明,章超桦,等.珍珠龙胆石斑鱼肌肉营养成分与挥发性风味成分的分析与评价[J].广东海洋大学学报,2018,38(1):39-46.FAN Xiu-ping,QIN Xiao-ming,ZHANG Chao-hua,et al.Nutritional and volatile flavor components of dorsal and ventral muscle from hybrid grouper(Epinephelus fuscoguttatus♀ × E.lanceolatus♂)[J].Journal of Guangdong Ocean University,2018,38(1):39-46.
[21] 白婵,熊光权,黄敏,等.丁香酚麻醉斑点叉尾鮰在不同无水保活温度下的生理生化变化[J].渔业现代化,2019,46(1):60-66.BAI Chan,XIONG Guang-quan,HUANG Min,et al.Study on physiological-biochemical changes of Ictalurus punctatus anesthetized by eugenol at different waterless preservation temperatures[J].Fishery Modernization,2019,46(1):60-66.
[22] 吴胜泽,熊波,黄钟标,等.无水保活下鲫鱼生理生化指标的变化[J].食品安全质量检测学报,2018,9(15):3 992-3 998.WU Sheng-ze,XIONG Bo,HUANG Zhong-biao,et al.Changes of physiological and biochemical properties of Crucianauratus waterless keep-alive during the transportation[J].Journal of Food Safety and Quality,2018,9(15):3 992-3 998.
[23] 李宁,白婵,熊光权,等.无水保活时间对斑点叉尾鮰血液生化和肌肉品质的影响[J].现代食品科技,2018,34(12):74-81.LI Ning,BAI Chan,XIONG Guang-quan,et al.Effects of waterless keep alive time on physiological and biochemical index of ictalurus punctatus[J].Modern Food Science and Technology,2018,34(12):74-81.
[24] 白艳龙.黄颡鱼保活技术研究[D].上海:上海海洋大学,2013:40-41.BAI Yan-long.Study on the keep-alive technology for yellow fish[D].Shanghai:Shanghai Ocean University,2013:40-41.
[25] JO Y J,JUNG K H,LEE M Y,et al.Effect of high-pressure short-time processing on the physicochemical properties of abalone(Haliotis discus hannai)during refrigerated storage[J].Innovative Food Science and Emerging Technologies,2014,23:33-38.
[26] 聂小宝,吴波,侯梦然,等.无水保活条件下不同温度对鲟鱼生理生化指标的影响[J].食品科技,2017,42(12):147-152.NIE Xiao-bao,WU Bo,HOU Meng-ran,et al.Effects of different temperatures on physiological and biochemical indexes of sturgeon under no water preservation[J].Food Science and Technology,2017,42(12):147-152.
[27] HSIAO-LI C,YEN-TE H,CHIEN-CHAO C,et al.Metabolomics characterization of energy metabolism reveals glycogen accumulation in gut-microbiota-lacking mice[J].The Journal of Nutritional Biochemistry,2012,23(7):752-758.
[28] REFAEY M M,TIAN X,TANG R,et al.Changes in physiological responses,muscular composition and flesh quality of channel catfish Ictalurus punctatus suffering from transport stress[J].Aquaculture,2017,478:9-15.
[29] 武桂新,严翊,冯炜权.壳聚糖对无氧训练大鼠糖代谢的影响[J].体育科学,2002,22(1):91-93.WU Gui-xin,YAN Yi,FENG Wei-quan.The effects of chitosan on carbohydrates metabolism in rats during anaerobic training[J].Sports Science,2002,22(1):91-93.
[30] MORAES G,AVILEZ I M,ALTRAN A E,et al.Biochemical and hematological responses of the banded knife fish Gymnotus carapo(Linnaeus,1758)exposed to environmental hypoxia[J].Brazilian Journal of Biology,2017,62(4a):633.
[31] 米红波.鲫鱼和中国对虾的无水保活及冰温保鲜技术研究[D].杭州:浙江大学,2014:50-51.MI Hong-bo.Study on waterless preservation and controlled freezing-point storage of crucian carp and Chinese white shrimp(Fenneropenaeus chinensis)[D].Hangzhou:Zhejiang University,2014:50-51.
[32] 刘寒,钱磊,张志军,等.生物保鲜剂应用于水产品保鲜的研究进展[J].食品研究与开发,2019,40(18):208-212.LIU Han,QIAN Lei,ZHANG Zhi-jun,et al.Research progress on application of biological preservatives in aquatic products[J].Food Research and Development,2019,40(18):208-212.
[33] HEINRICHS-CALDAS W,CAMPOS D F,PAULA-SILVA M N,et al.Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar,Astronotus crassipinnis[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2019,227:31-38.
[34] WU Yi-wen,YOU Xiao-peng,SUN Wei-qing,et al.Insight into acute heat stress on meat qualities of rainbow trout(Oncorhynchus mykiss)during short-time transportation[J].Aquaculture,2021,543:737013.
[35] LI F J,LIN X,LIN S M,et al.Effects of dietary fish oil substitution with linseed oil on growth,muscle fatty acid and metabolism of tilapia(Oreochromis niloticus)[J].Aquaculture Nutrition,2016,22(3):499-508.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.