Optimizations of extraction and separation process of saponins and in vitro antioxidant activity from the female gonad of Apostichopus japonicus

Authors

ZHONG Jing-shi, College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306 , China ；Shandong Marine Resource and Environment Research Institute, Yantai, Shandong 264006 , China
ZHANG Jian, Shandong Marine Resource and Environment Research Institute, Yantai, Shandong 264006 , China
LIU Fang, Shandong Marine Resource and Environment Research Institute, Yantai, Shandong 264006 , China
WANG Gong-ming, Shandong Marine Resource and Environment Research Institute, Yantai, Shandong 264006 , China
WANG Yi-xin, College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306 , China ；Shandong Marine Resource and Environment Research Institute, Yantai, Shandong 264006 , China

Abstract

Objective: This study aimed to optimize the ultrasonic-assisted extraction technology and HSCCC separation process of saponins from the female gonad of Apostichopus japonicus and to explore their antioxidant activities. Methods: Extraction time, ethanol concentration, ultrasonic time, and solid-to-solvent ratio as the influencing factors were considered in this study, and the extraction process of saponin was optimized using the response surface methodology. The ratio of solvent systems, the volume flow of mobile phases, and the rotation speeds of HSCCC were optimized. The DPPH radical and ABTS radical scavenging experiments were conducted to evaluate the antioxidant activities of the crude extracts and the separated fractions. Results: The optimal extraction condition of the crude saponin were extraction time of 7 d, ethanol concentration of 83%, ultrasound time of 33 min, and solid-to-solvent ratio of 1∶20 (g/mL). Under these conditions, the extraction rate of saponins was (0.66 ± 0.02)%. Components 1 and 2 were separated from the crude saponin by HSCCC with V_acetate∶V_butanol∶V_methanol∶V_water=2.0∶3.0∶0.2∶4.8, 2 mL/min volume flow, and 800 r/min rotation speed. The DPPH radical scavenging rate of the crude saponin, component 1, and component 2 were (36.23±0.55)%, (35.07±0.20)%, and (38.40±0.14)%, respectively, while the ABTS radical scavenging rate were (17.55±0.42)%, (24.49±0.50)% and (33.65±0.34)%, respectively. Conclusion: A higher yield of crude saponin from the female gonad of Apostichopus japonicus with better separation was obtained after process optimization. Compared with the crude saponin, the antioxidant activity of component 2 was improved after HSCCC separation.

Publication Date

6-30-2022

First Page

149

Last Page

156,245

DOI

10.13652/j.spjx.1003.5788.2022.90182

Recommended Citation

Jing-shi, ZHONG; Jian, ZHANG; Fang, LIU; Gong-ming, WANG; and Yi-xin, WANG (2022) "Optimizations of extraction and separation process of saponins and in vitro antioxidant activity from the female gonad of Apostichopus japonicus," Food and Machinery: Vol. 38: Iss. 5, Article 28.
DOI: 10.13652/j.spjx.1003.5788.2022.90182
Available at: https://www.ifoodmm.cn/journal/vol38/iss5/28

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