•  
  •  
 

Abstract

Objective: A process was developed for the purification of bran polyphenols from HPD 600 macroporous resin. The purity and antioxidant activity of the extracts were analyzed before and after purification, and the polyphenol fractions in the purified products were characterized. Methods: The bound polyphenols in wheat bran were extracted by alkali hydrolysis, and the dynamic adsorption-desorption conditions of the macroporous resin were optimized by single-factor test to determine the optimal purification process. The antioxidant activity fraction was determined by ABTS and DPPH radical scavenging rate. The characterization of the polyphenol fraction in the purified product was completed by UPLC-MS/MS. Results: Under optimal process conditions, the purity of the polyphenols and the antioxidant activity of the purified product were significantly increased. The purified products contained mainly nine polyphenolic compounds, in order of retention time: p-hydroxybenzoic acid, caffeic acid, vanillin, p-coumaric acid, trans-ferulic acid and salicylic acid. Conclusion: The macroporous resin purification process is effective in enriching bran polyphenols and the resulting purified product has a significantly higher antioxidant capacity, while maintaining the richness of the polyphenolic species.

Publication Date

6-30-2022

First Page

173

Last Page

178,242

DOI

10.13652/j.spjx.1003.5788.2022.90028

References

[1] ASHUN Chaudhary,VIVEK S Jaswal,SONIKA Choudhary,et al.Ferulic acid:A promising therapeutic phytochemical and recent patents advances[J].Recent Patents on Inflammation & Allergy Drug Discovery,2019,13(2):115-123.
[2] NILOOFAR Kahkeshani,FATEMEH Farzaei,MARYAM Fotouhi,et al.Pharmacological effects of gallic acid in health and diseases:A mechanistic review[J].Iranian Journal of Basic Medical Sciences,2019,22(3):225-237.
[3] PEI Ke-han,OU Juan-ying,HUANG Jun-qing,et al.p-Coumaric acid and its conjugates:Dietary sources,pharmacokinetic properties and biological activities[J].Journal of the Science of Food and Agriculture,2016,96(9):2 952-2 962.
[4] TRUST Beta,SHIN Nam,JIM E Dexter,et al.Phenolic content and antioxidant activity of pearled wheat and roller-milled fractions[J].Cereal Chemistry,2004,82(4):390-393.
[5] BEATRIZ A Acosta-Estrada,JANET A Gutiérrez-Uribe,SERGIO O Serna-Saldívar.Bound phenolics in foods,a review[J].Food Chemistry,2014,152:46-55.
[6] KIM Kyung-Hee,TSAO Rong,YANG Raymond,et al.Phenolic acid profiles and antioxidant activities of wheat bran extracts and the effect of hydrolysis conditions[J].Food Chemistry,2006,95(3):466-473.
[7] TANG Yao,ZHANG Bing,LI Xi-hong,et al.Bound phenolics of quinoa seeds released by acid,alkaline,and enzymatic treatments and their antioxidant and α-glucosidase and pancreatic lipase inhibitory effects[J].Journal of Agricultural and Food Chemistry,2016,64(8):1 712-1 719.
[8] 陶莎,黄英,康玉凡,等.大孔吸附树脂分离纯化红小豆多酚工艺及效果[J].农业工程学报,2013,29(23):276-285.TAO Sha,HUANG Ying,KANG Yu-fan,et al.Technology of separation and purification and its efficiency of adzuki bean polyphenols with macroporous adsorption resins[J].Transactions of the Chinese Society of Agricultural Engineering,2013,29(23):276-285.
[9] 张子东,张圆圆,孟永斌,等.沙枣果总黄酮类化合物的富集研究[J].植物研究,2018,38(3):467-474.ZHANG Zi-dong,ZHANG Yuan-yuan,MENG Yong-bin,et al.Flavonoidsenrichment from elaeagnus angustifolia fruit[J].Bulletin of Botanical Research,2018,38(3):476-474.
[10] VERMA B,HUCL P,CHIBBAR R N.Phenolic acid composition and antioxidant capacity of acid and alkali hydrolysed wheat bran fractions[J].Food Chemistry,2009,116(4):947-954.
[11] REBECA López-Froilán,BLANCA Hernández-Ledesma,MONTAÑA Cámara,et al.Evaluation of the antioxidant potential of mixed fruit-based beverages:A new insight on the folin-ciocalteu method[J].Food Analytical Methods,2018,11(10):2 097-2 906.
[12] 曾棋平,吴坤林,陈锦珊.大孔吸附树脂纯化紫茶总多酚的工艺优化研究[J].药学实践杂志,2021,39(1):77-81.ZENG Qi-ping,WU Kun-lin,CHEN Jin-shan.Optimization of total polyphenol purification from purple tea by microporous resin[J].Journal of Pharm Aceutical Practice,2021,39(1):77-81.
[13] 陈明威,魏明,陶良凡,等.大孔树脂分离纯化霍山石斛多酚及其抗氧化活性研究[J].食品与机械,2020,36(11):148-153.CHEN Ming-wei,WEI Ming,TAO Fan-liang,et al.Purification of polyphenols from Dendrobium huoshanense by macroporous resin and its antioxidant activity[J].Food & Machinery,2020,36(11):148-153.
[14] PAMELA Raquel Rivera-Tovar,MARÍA Dolores Torres,CONRADO Camilo,et al.Multi-response optimal hot pressurized liquid recovery of extractable polyphenols from leaves of maqui(Aristotelia chilensis [Mol.] Stuntz)[J].Food Chemistry,2021,357:129729.
[15] RICARDO Iván González-Vega,JOSÉ Luis Cárdenas-López,JOSÉ Antonio López-Elías,et al.Optimization of growing conditions for pigments production from microalga Navicula incerta using response surface methodology and its antioxidant capacity[J].Saudi Journal of Biological Sciences,2021,28(2):1 401-1 416.
[16] BRAND-WILLIAMS W,CUVELIER M E,BERSET C.Use of a free radical method to evaluate antioxidant activity[J].LWT-Food Science and Technology,1995,28(1):25-30.
[17] MOHAMED Saleh A,KHAN Jalaluddin A.Antioxidant capacity of chewing stick miswak Salvadora persica[J].BMC Complementary and Alternative Medicine,2013,13(1):40-45.
[18] 马景蕃,谢晓倩,杨昊坤,等.大孔树脂纯化白背天葵多酚及其体外抗氧化研究[J].热带作物学报 2021,42(2):575-582.MA Jing-fan,XIE Xiao-qian,YANG Hao-kun,et al.Purification of Gynura formosana Kitam polyphenols by macroporous resin and its antioxidant activity in vitro[J].Chinese Journal of Tropical Crops,2021,42(2):575-582.
[19] 袁欢,彭莉莎,孙梦瑶,等.大孔树脂对二角菱壳多酚的吸附及解吸性能研究[J].食品与机械,2021,37(4):47-52.YUAN Huan,PENG Li-sha,SUN Meng-yao,et al.Study on absorption and desorption properties of macroporous resin for pericarps of Trapa bispinosa Roxb[J].Food & Machinery,2021,37(4):47-52.
[20] JIA Guang-tao,LU Xiu-yang.Enrichment and purification of madecassoside and asiaticoside from Centella asiatica extracts with macroporous resins[J].Journal of Chromatography A,2008,1 193(1/2):136-141.
[21] XI Li-sha,MU Tai-hua,SUN Hong-nan.Preparative purification of polyphenols from sweet potato(Ipomoea batatas L.)leaves by AB-8 macroporous resins[J].Food Chemistry,2015,172:166-174.
[22] 王兴明,康明.基础有机化学[M].北京:科学出版社,2012:208-209.WANG Xing-ming,KANG Ming.Basic organic chemistry[M].Beijing:Science Press,2012:208-209.
[23] 于世林.高效液相色谱方法及应用[M].3版.北京:化学工业出版社,2018:120-123.YU Shi-lin.Methods and applications of high-performance liquid chromatography[M].3rd ed.Beijing:Chemical Industry Press,2018:120-123.
[24] ONOFREJOVÁ L,VAŠÍCKOVÁ J,KLEJDUS B,et al.Bioactive phenols in algae:The application of pressurized-liquid and solid-phase extraction techniques[J].Journal of Pharmaceutical and Biomedical Analysis,2010,51(2):464-470.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.